The Evaluation of the Two-Stage Prothrombin Assay

H. C Hemker; P. W Hemker; K. v. d Torren; P. P Devilee; W. Th Hermens; E. A Loeliger

doi:10.1055/s-0038-1654328

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1971; 25(03): 545-554
DOI: 10.1055/s-0038-1654328

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

The Evaluation of the Two-Stage Prothrombin Assay

H. C Hemker

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands

,

P. W Hemker

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands

,

K. v. d Torren

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands

,

P. P Devilee

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands

,

W. Th Hermens

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands

,

E. A Loeliger

¹Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Departments of Haematology and Cardiology, University Hospital, Leiden, The Netherlands

› Author Affiliations

Abstract

Summary

It is shown that (and why) the traditional ways of evaluating a thrombin generation curve do not give acceptable estimations of the prothrombin content of the sample. An alternative method is shown to meet the requirements better; it consists of drawing the thrombin generation curve on a semi-logarithmic graph, and extrapolating the descending slope to zero time. This gives a thrombin concentration representing the prothrombin concentration of the original sample.

Full Text

References

References
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