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Thromb Haemost 1959; 03(02): 194-213
DOI: 10.1055/s-0038-1654382
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

On the Purification and Conversion of Human Prothrombin

F Streuli
1   Coagulation Laboratory (Prof. F. Koller) of the Department of Medicine (Prof. P. H. Rossier) University of Zurich, Switzerland
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Publication Date:
08 June 2018 (online)

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Summary

A combined chemical and immunological method for the purification of human prothrombin is described. After isoelectric precipitation a purified prothrombin is obtained. Its activity per mg of protein is 210 folds higher than that of plasma. This prothrombin and the prothrombin purified on a BaSO4 column are not converted into thrombin in a 25% sodium-citrate solution. No thrombin is formed by the addition of calcium ions and brain thromboplastin. For its conversion, a complete clotting system (extrinsic or intrinsic) is necessary. Our experiments present no evidence for the hypothesis that Factors VII, IX, PPA and Stuart-Prower are prothrombin derivatives.

The conversion of prothrombin to thrombin by means of isolated blood thromboplastin is studied. The results still do not permit one to decide whether the reaction is stoichiometric or enzymatic.


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  • References

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    PubMedGoogle Scholar
  • 2 Bachmann F, Duckert F, Koller F. The Stuart-Prower Factor Assay and its Clinical Significance. Thromb. Diath. haem 1958; 2: 24
    PubMedGoogle Scholar
  • 3 Bell W. N, Alton H. G. A Brain Extract as a Substitute for Platelet Suspensions in the Thromboplastin Generation Test. Nature 1954; 174: 880
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    Google Scholar
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  • 7 Duckert F, Koller F, Matter M. Purification and Physiological Properties of Factor VII from Plasma and Serum. Separation from Prothrombin. Proc. Soc. Exp. Biol. and Med 1953; 82: 259
    PubMedGoogle Scholar
  • 8 Duckert F, Flückiger P, Isenschmid H, Matter M, Vogel-Meng J, Koller F. A Modification of the Thromboplastin Generation Test. Acta haemat 1954; 12: 197
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  • 9 Edsall J. T. The Plasma Proteins and Their Fractionation. Advanc. Protein Chem 1947; III: 383
    PubMedGoogle Scholar
  • 10 Fisch U. Über einen neuen Akzelerator der Blutthrombokinasebildung. Thromb. Diath. haem 1958; 2: 60
    PubMedGoogle Scholar
  • 11 Folin O, Ciocalteu V. Dosage de la Tyrosine. J. Biol. Chem 1927; 73: 627
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    PubMedGoogle Scholar
  • 14 Kappeier R. Das Verhalten von Faktor V im Serum unter normalen und pathologischen Bedingungen. Zschr. f. klin. Med 1955; 153: 103
    PubMedGoogle Scholar
  • 15 Koller F, Loeliger A, Duckert F. Experiments on a New Clotting Factor (Factor VII). Acta haemat 1951; 6: 1
    CrossrefPubMedGoogle Scholar
  • 16 Laki K. et al. The Amino Acid Composition of Bovine Prothrombin. Arch. Biochem. and Biophysics 1954; 49: 276
    CrossrefPubMedGoogle Scholar
  • 17 Lasch H. G, Roka L. Zur Prothrombinbildung in der Leber. Hoppe-Seylers Zschr. f. physiol. Chem 1953; 294: 30
    PubMedGoogle Scholar
  • 18 Lewis J. H, Didisheim P. Production of Anti-Human PTC and Anti-Human Proconvertin in Rabbits. Proc. Soc. Exp. Biol. and Med 1956; 93: 429
    PubMedGoogle Scholar
  • 19 Oncley J. L, Melin M, Richert D. A, Cameron J. W, Gross Jr. P. M, Amer J. The Separation of the Antibodies, Isoagglutinins, Prothrombin, Plasminogen, — Lipoprotein into Subfractions of Human Plasma. J. Am. Chem. Soc 1949; 71: 541
    CrossrefPubMedGoogle Scholar
  • 20 Quick A. J. The Prothrombin in Haemophilia and in Obstructive Jaundice. J. Biol. Chem 1935; 109: LXXIII
    PubMedGoogle Scholar
  • 21 Quick A. J, Stefanini M. The State of Component A (Prothrombin) in Human Blood. J. Lab. Clin. Med 1949; 34: 1203
    PubMedGoogle Scholar
  • 22 Schmidt A. Zur Blutlehre. Leipzig. 1893
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  • 23 Seegers W. H, McClaughry R. L, Fahey J. L. Some Properties of Purified Prothrombin and its Activation with Sodium Citrate. Blood 1950; 5: 421
    PubMedGoogle Scholar
  • 24 Seegers W. H. The Purification of Prothrombin. Rec. Chem. Progress 1952; 13: 143
    PubMedGoogle Scholar
  • 25 Seegers W. H, Alkjaersig N, Johnson S. A. Formation of Autopro-thrombin in Solutions Containing Purified Prothrombin and Purified Platelet Factor 3. Am. J. Physiol 1955; 181: 589
    PubMedGoogle Scholar
  • 26 Seegers W. H, Johnson S. A. Conversion of Prothrombin to Autoprothrombin II (Platelet Cofactor II) and its Relation to the Blood Clotting Mechanisms. Am. J. Physiol 1956; 184: 259
    PubMedGoogle Scholar
  • 27 Zollinger W. Diss. Zurich 1958 (to be published)
    PubMed

  • References

  • 1 Alkjaersig N, Seegers W. H. Formation of Prothrombin From Autoprothrom-bin by Means of Liver Mitochondria. Am. J. Physiol 1955; 183: 111
    PubMedGoogle Scholar
  • 2 Bachmann F, Duckert F, Koller F. The Stuart-Prower Factor Assay and its Clinical Significance. Thromb. Diath. haem 1958; 2: 24
    PubMedGoogle Scholar
  • 3 Bell W. N, Alton H. G. A Brain Extract as a Substitute for Platelet Suspensions in the Thromboplastin Generation Test. Nature 1954; 174: 880
    CrossrefPubMedGoogle Scholar
  • 4 Biggs R, Douglas A. S, MacFarlane R. G. The Thromboplastin Generation Test. J. Physiol 1953; 119: 89
    CrossrefPubMedGoogle Scholar
  • 5 Biggs R, MacFarlane R. G. Human Blood Coagulation and its Disorders. Black-well Scientific Publications; Oxford: 2nd Edition. 1957: 302
    Google Scholar
  • 6 Clauss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta haemat 1957; 17: 4
    PubMedGoogle Scholar
  • 7 Duckert F, Koller F, Matter M. Purification and Physiological Properties of Factor VII from Plasma and Serum. Separation from Prothrombin. Proc. Soc. Exp. Biol. and Med 1953; 82: 259
    PubMedGoogle Scholar
  • 8 Duckert F, Flückiger P, Isenschmid H, Matter M, Vogel-Meng J, Koller F. A Modification of the Thromboplastin Generation Test. Acta haemat 1954; 12: 197
    CrossrefPubMedGoogle Scholar
  • 9 Edsall J. T. The Plasma Proteins and Their Fractionation. Advanc. Protein Chem 1947; III: 383
    PubMedGoogle Scholar
  • 10 Fisch U. Über einen neuen Akzelerator der Blutthrombokinasebildung. Thromb. Diath. haem 1958; 2: 60
    PubMedGoogle Scholar
  • 11 Folin O, Ciocalteu V. Dosage de la Tyrosine. J. Biol. Chem 1927; 73: 627
    PubMedGoogle Scholar
  • 12 Geiger M, Duckert F, Koller F. Quantitative Bestimmungen von Faktor VIII und Faktor IX bei Blutersippen. 5. Congr. Europ. Soc. Haemat. Springer Verlag; Heidelberg: 1956
    Google Scholar
  • 13 Horejsi J, Smetana R. Isolation of Gamma-Globulins from Blood Serum by Rivanol. Acta Med. Scand 155 Fasc I 1956;
    PubMedGoogle Scholar
  • 14 Kappeier R. Das Verhalten von Faktor V im Serum unter normalen und pathologischen Bedingungen. Zschr. f. klin. Med 1955; 153: 103
    PubMedGoogle Scholar
  • 15 Koller F, Loeliger A, Duckert F. Experiments on a New Clotting Factor (Factor VII). Acta haemat 1951; 6: 1
    CrossrefPubMedGoogle Scholar
  • 16 Laki K. et al. The Amino Acid Composition of Bovine Prothrombin. Arch. Biochem. and Biophysics 1954; 49: 276
    CrossrefPubMedGoogle Scholar
  • 17 Lasch H. G, Roka L. Zur Prothrombinbildung in der Leber. Hoppe-Seylers Zschr. f. physiol. Chem 1953; 294: 30
    PubMedGoogle Scholar
  • 18 Lewis J. H, Didisheim P. Production of Anti-Human PTC and Anti-Human Proconvertin in Rabbits. Proc. Soc. Exp. Biol. and Med 1956; 93: 429
    PubMedGoogle Scholar
  • 19 Oncley J. L, Melin M, Richert D. A, Cameron J. W, Gross Jr. P. M, Amer J. The Separation of the Antibodies, Isoagglutinins, Prothrombin, Plasminogen, — Lipoprotein into Subfractions of Human Plasma. J. Am. Chem. Soc 1949; 71: 541
    CrossrefPubMedGoogle Scholar
  • 20 Quick A. J. The Prothrombin in Haemophilia and in Obstructive Jaundice. J. Biol. Chem 1935; 109: LXXIII
    PubMedGoogle Scholar
  • 21 Quick A. J, Stefanini M. The State of Component A (Prothrombin) in Human Blood. J. Lab. Clin. Med 1949; 34: 1203
    PubMedGoogle Scholar
  • 22 Schmidt A. Zur Blutlehre. Leipzig. 1893
    Google Scholar
  • 23 Seegers W. H, McClaughry R. L, Fahey J. L. Some Properties of Purified Prothrombin and its Activation with Sodium Citrate. Blood 1950; 5: 421
    PubMedGoogle Scholar
  • 24 Seegers W. H. The Purification of Prothrombin. Rec. Chem. Progress 1952; 13: 143
    PubMedGoogle Scholar
  • 25 Seegers W. H, Alkjaersig N, Johnson S. A. Formation of Autopro-thrombin in Solutions Containing Purified Prothrombin and Purified Platelet Factor 3. Am. J. Physiol 1955; 181: 589
    PubMedGoogle Scholar
  • 26 Seegers W. H, Johnson S. A. Conversion of Prothrombin to Autoprothrombin II (Platelet Cofactor II) and its Relation to the Blood Clotting Mechanisms. Am. J. Physiol 1956; 184: 259
    PubMedGoogle Scholar
  • 27 Zollinger W. Diss. Zurich 1958 (to be published)
    PubMed

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