Thromb Haemost 1963; 10(02): 295-308
DOI: 10.1055/s-0038-1654785
Originalarbeiten — Original Article — Travaux Originaux
Schattauer GmbH

Changes in Blood Coagulation and Fibrinolysis in Rats Fed Atherogenic Diets[*]

Clarence Merskey
1   Department of Medicine and the Unit for Research in Ageing Albert Einstein College of Medicine; Yeshiva University and the Bronx Municipal Hospital Center, Bronx 61, N. Y
,
Herbert Wohl
1   Department of Medicine and the Unit for Research in Ageing Albert Einstein College of Medicine; Yeshiva University and the Bronx Municipal Hospital Center, Bronx 61, N. Y
› Author Affiliations
Further Information

Publication History

Publication Date:
22 June 2018 (online)

Summary

1. Groups of rats were fed thrombogenic diets and the effects on blood coagulation and fibrinolysis assessed.

2. Animals fed a diet containing cholesterol, thiouracil and cholic acid developed high levels of coagulation factors I, II, V, VII—X, VIII, IX and X.

3. Animals fed a similar diet with additional 40% beef fat developed even greater elevation of V, VII—X, VIII and X, similar elevation of factor II, and lesser (but still significant) elevation of factors I and IX. In addition marked elevation of blood platelets occurred.

4. Euglobulin lysis time of the group not fed the additional fat was longer than in controls. Significant prolongation of euglobulin lysis time was not found in the group fed additional fat.

5. If the increased levels of plasma fibrinogen were taken into account, it was found that a larger amount of fibrin was lysed per unit time in the euglobulin lysis test with plasma from rats fed either atherogenic diet compared with controls.

6. Defective thromboplastin generation was present in both groups of rats fed an atherogenic diet. The defect was present in the serum and was not due to lack of a factor required for thromboplastin generation. An inhibitor was present in the serum which was capable of preventing the action of normal serum.

7. No good correlation was found between the occurrence of changes in blood coagulation or fibrinolysis and the presence or absence of thrombosis and infarction.

8. The exact cause of these anomalies remains unexplained, as does the cause of the thrombosis in these animals. Starvation per se does not account for these abnormal findings. They could not adequately be explained on the basis of “hypercoagulability” of the blood.

* This investigation was supported by U.S.P.H.S. grants HE 06191, H 3838, M 2562, A 2965.


 
  • References

  • 1 Wissler R. W, Eilert M. L, Schroeder M. A, Cohen L. Production of lipomatous and atheromatous arterial lesions in the albino rat. Arch. Path. 57: 333 1954;
  • 2 Fillios L. C, Andrus S. B, Mann G. V, Stare F. J. Experimental production of gross atherosclerosis in the rat. J. exp. Med. 104: 539 1956;
  • 3 Wilgram G. F. Experimental atherosclerosis and cardiac infarcts in rats. J. exp. Med. 109: 293 1959;
  • 4 Thomas W. A, Hartroft W. S. Myocardial infarction in rats fed diets containing high fat, cholesterol, thiouracil and sodium cholate. Circulation. 19: 65 1959;
  • 5 Davidson E, Howard A. N, Gresham G. A. The nature of the coagulation defect in rats fed diets which produce thrombosis or experimental atherosclerosis. Brit. J. exp. Path. 43: 166 1962;
  • 6 Kudrjashoy B. A, Bazasian G. G, Sytina N. P, Andreenko G. V. Experimental pre-thrombotic state of an organism resulting from the dysfunction of the physiological anticoagulating system induced by an atherogenic diet. Nature (Lond.). 189: 67 1961;
  • 7 Naimi S, Goldstein R, Nothman M. M, Wilgram G. F, Proger S. Cardiovascular lesions and changes in blood coagulation and fibrinolysis associated with diet-induced lipemia in the rat. J. clin. Invest. 41: 1708 1962;
  • 8 Fisher L. M, Kupfer H. G, Kagan E. Coagulation changes induced in rats fed a thrombogenic diet. Fed. Proc. 21: 59 1962;
  • 9 Davidson E, Howard A. N, Gresham G. A. Blood coagulation studies in rats given diets which produce thrombosis or atherosclerosis. Brit. J. exp. Path. 42: 195 1961;
  • 10 Merskey C, Wohl H, Oka M. Effect of atherogenic diet on blood coagulation and fibrinolysis in the rat. Fed. Proc. 21: 58 1962;
  • 11 Spaet T. H, Cintron J, Kropatkin M. A technique for determining whole blood clotting times in plastic tubes. J. Lab. clin. Med. 54: 467 1959;
  • 12 Quick A. J. The prothrombin in hemophilia and in obstructive jaundice. J. biol. Chem.. 109. IXXiii| 1935
  • 13 Hjort P, Rapaport S. I, Owren P. A. A simple, specific one-stage prothrombin assay using Russell’s viper venom in cephalin suspension. J. Lab. clin. Med. 46: 89 1955;
  • 14 Ware A. G, Seegers W. H. Two-stage procedure for the quantitative determination of prothrombin concentration. Amer. J. clin. Path. 19: 471 1949;
  • 15 Owren P. A, Aas K. The control of dicumarol therapy and the quantitative determination of prothrombin and proconvertin. Scand. J. clin. Lab. Invest. 03: 201 1951;
  • 16 Denson K. W. The specific assay of Prower-Stuart factor and factor VII. Acta haemat. (Basel). 25: 105 1961;
  • 17 Quick A. J. The assay and properties of labile factor (Factor V). J. clin. Path. 13: 457 1960;
  • 18 Pool J. G, Robinson J. Assay of plasma antihaemophilic globulin (AHG). Brit. J. Haemat. 05: 17 1959;
  • 19 Ratnoff O. D, Menzie C. A new method for the determination of fibrinogen in small samples of plasma. J. Lab. clin. Med. 37: 316 1951;
  • 20 Brecher G, Cronkite E. P. Morphology and enumeration of human blood platelets. J. appl. Physiol. 03: 365 1950;
  • 21 Biggs R, Macfarlane R. G. Human blood coagulation and its disorders. 3rd. Ed. F.A. Davis Company; Philadelphia: 1962
  • 22 Bell W. N, Alton H. G. A brain extract as a substitute for platelet suspensions in the thromboplastin generation test. Nature (Lond.). 174: 880 1954;
  • 23 Blix S. Studies on the fibrinolytic system in the euglobulin fraction of human plasma. Scand. J. clin. Lab. Invest. (Suppl.). 13: 58 1961;
  • 24 Lackner H, Goosen C. C. Fibrinolytic activity of blood: Photographic determination of clot lysis time. Acta haemat. (Basel). 22: 58 1959;
  • 25 Quick A. J. Oral contraception and coagulability. Brit. med. J. I: 1604 1963;