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DOI: 10.1055/s-0038-1654786
Method for Determining ε-Aminocaproic Acid (EACA) in Blood Plasma, Based on the Antifibrinolytic Properties of this Compound
Publication History
Publication Date:
22 June 2018 (online)
Summary
A method was elaborated for determining e-aminocaproic acid (EACA) in blood plasma, based upon this compound’s antifibrinolytic activity.
Oxalated plasma was deproteinized in a boiling water-bath using trichloracetic acid. The latter was removed from plasma filtrate by means of ether extraction and the effect of the deproteinized extract on clot fibrinolysis time under standard conditions was tested. There was found a linear correlation of the logarithm of clot lysis time with EACA concentration.
The method appears to be fast and reliable, making possible serial determinations of the EACA level in blood plasma, the error being within the limits of 3 percent. It is suitable for the EACA level in plasma exceeding 2 mgVo.
The method is most specific. Various amino acids appearing naturally (except cysteine) do not interfere with determinations.
The EACA metabolism in vivo may be successfully studied using the above method.
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References
- 1 Ablondi F. B, Hagan J. J, Philips M, De Renzo E. C. Inhibition of plasmin, trypsin and the streptokinase-activated fibrinolytic system by 8-aminocaproic acid. Arch. Biochem. 82: 153 1959;
- 2 Alkjaersig N, Fletcher A. P, Sherry S. e-aminocaproic acid: an inhibitor of plasminogen activation. J. biol. Chem. 234: 832 1959;
- 3 Celander D. R, Dale Messer, Guest M. M. The fibrinolytic system: a review of its therapeutic significance and control with particular emphasis on its inhibition by e-aminocaproic acid. Tex. Rep. Biol. Med. 19: 16 1961;
- 4 Buyske D. A, Colucci D. Spectrophotometric method for determination of e-aminocaproic acid. Personal communication of Dr. M.W. Sweeney..
- 5 Johnson A. J, Skoza L. Chemical determination of epsilon-amino-caproic acid, an inhibitor of plasminogen activation. Fed. Proc. 20 nr. 1 59 1961;
- 6 Lewis J. M, Ferguson J. H. Partial purification of human prothrombin and proconvertin; their characteristics and interaction. J. clin. Invest. 32: 915 1953;
- 7 McNicol G. P, Fletcher A. P, Alkjaersig N, Sherry S. The use of e-aminocaproic acid, a potent inhibitor of fibrinolytic activity, in the management of postoperative hematuria. J. Urol. (Baltimore.) 86: 829 1961;
- 8 McNicol G. P, Fletcher A. P, Alkjaersig N, Sherry S. Plasma amino acid chromatography with ion exchange resin loaded paper: Assay of 8-aminocaproic acid. J. clin. Lab. Med. 59: 7 1962;
- 9 Nilsson I. M, Björkman S. E, Andersson L. Clinical experiences with 8-aminocaproic acid (EACA) as an antifibrinolytic agent. Acta med. scand. 170: 487 1961;
- 10 Okamoto S. Plasmin and antiplasmin. Their pathologic physiology. Keio J. Med. 08: 211 1959;
- 11 Sato S, Ishibashi Y, Endo T, Watanabe T, Nakajima K. Clinical use of e-amino-n-caproic acid on metropathia hemorrhagica. Keio J. Med. 08: 267 1959;
- 12 Sjoerdsma A, Nilsson I. M. Aliphatic amino compounds as inhibitors of plasminogen activation. Proc. Soc. exp. Biol. (N. Y.) 103: 533 1960;
- 13 Sjoerdsma A, Hanson A. Determination of e-aminocaproic acid in urine by means of high voltage paper electrophoresis. Acta chem. scand. 13: 2150 1959;
- 14 Stegemann H. Die Gesetzmäßigkeiten der Aminosäuretrennung an Austauschersäulen bei kontinuierlicher Elution. Hoppe-Seylers 2. physiol. Chem. 319: 87 1960;
- 15 Stegemann H. Bestimmung von Aminosäuren mit dithionitroduziertem Ninhydrin. Hoppe-Seylers 2. physiol. Chem. 319: 102 1960;
- 16 Thomas K, Stalder K, Stegemann H. Stoffwechselversuche mit e-Amino-capronsäure. Hoppe-Seylers 2. physiol. Chem. 317: 267 1959;