The Action on Blood Clotting of Different Lipoid Fractions of Beef Brain* (Methods of Fractionization and Chromatographic Isolation Procedure of a Phospholipid Anticoagulant)

M. J. P Kahn; M Hans; R. H Bourgain

doi:10.1055/s-0038-1654881

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1965; 14(03/04): 445-453
DOI: 10.1055/s-0038-1654881

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

The Action on Blood Clotting of Different Lipoid Fractions of Beef Brain* (Methods of Fractionization and Chromatographic Isolation Procedure of a Phospholipid Anticoagulant)

M. J. P Kahn

¹Laboratoire de Physiologie et Laboratoire de Pharmacognosie, Université Libre de Bruxelles

,

M Hans

¹Laboratoire de Physiologie et Laboratoire de Pharmacognosie, Université Libre de Bruxelles

,

R. H Bourgain

¹Laboratoire de Physiologie et Laboratoire de Pharmacognosie, Université Libre de Bruxelles

› Author Affiliations

Abstract

Summary

A method of fractionation and isolation of a lipid anticoagulant from beef brain has been described. This method is based on chemical and chromatographic techniques. The lipid anticoagulant was found to be an inositol monophosphatide containing two different fatty acids. The anticoagulant activity has been evidenced in the Russell-viper-venom test and is believed to interfere with the formation of the early intermediate products involving factor X.

Full Text

References

References
1 Chargaff E. Studies on the chemistry of blood coagulation IV-lipid inhibitors of blood clotting occuring in mammalian tissue. J. Biol. 121: 175 1937;
2 Tocantins L. M. Antithromboplastic activity of normal and hemophilic plasma. Fed. Proc. 01: 85 1942;
3 Tocantins L. M. Demonstration of antithromboplastic activity in normal and hemophilic plasma. Amer. J. Physiol. 139: 265 1943;
4 Tocantins L. M. Cephalin, protamin and the antithromboplastic activity of normal and hemophilic plasma. Proc. Soc. exp. Biol. (N. Y.) 54: 94 1943;
5 Tocantins L. M. Anticephalin activity and the prothrombin conversion rate of normal and hemophilic plasma. Proc. Soc. exp. Biol. (N.Y.) 55: 291 1944;
6 Overman P. S, Wright I. S. A new blood clotting inhibitor. J. Biol. Chem. 174: 759 1948;
7 Hecht E. New inhibitors of first stage of the blood-clotting process. Nature 167: 279 1951;
8 Hecht E, Ottens H. Die Bedeutung der Lipoide für Blutgerinnung. Acta haemat. (Basel.) 05: 265 1952;
9 Hecht E, Shapiro D. Sphingosine as an inhibitor of blood clotting. Science 125: 1041 1957;
10 Mushett C. W, Goldsmith DPJ, Kelly K. L. Studies on lipid anticoagulants. J. Biol. Chem. 211: 163 1954;
11 Johnson ShA, Seegers W. H. Studies on the plasma defect in hemophilia. Rev. Haematol. 9N° 3 bis: 529 1954;
12 Johnson ShA, Seegers W. H. Platelet cofactor I activity in the hemophilia. - Hemophilia and Hemophiloid Diseases. Edt. Brinkhous K. M. Univ. N. Carolina Press; 1957
13 Spaet ThHE, Gamier S. Inactivation of Thromboplastin in human blood. J. clin. Invest. 34: 1807 1955;
14 Silver M. J, Turner D. L, Tocantins L. M. Phospholipid antithromboplastin assay in vitro and evaluation in vivo of purified fractions. Amer. J. Physiol. 190: 8 1957;
15 Turner D. D, Silver M. J, Tocantins L. M. Phospholipid antithromboplastin. II Preparation and Properties. Arch. Biochem. Biophys. 77: 249 1958;
16 Ferguson J. H. Lipoids and blood platelets with references to blood coagulation and the hemorrhagic diseases. Univ. N. Carolina Press; 1960
17 Ferguson J. H. Coagulation balance between lipids and lipidinhibitors in the thromboplastic mechanism. Thrombos. Diathes. haemorrh. (Stuttg.) 07: 145 1961;
18 Turpini P, Mossa A, Cipolli P. L. La preparazione cromatografica die fosfolipidi su colonna di gel di silico. Bull, della Soc. Ital. Biol. 37: 1317 1961;
19 Bourgain P. Action anticoagulante d’un extrait lipoïdique de cerveau de boeuf — C. R. Soc. Biol. 150: 221 1956;
20 Bourgain P. Action inhibitrice d’un extrait lipoïdique de cerveau de boeuf vis à vis de la thromboplastine plasmatique. C. R. Soc. Biol. 151: 400 1957;
21 Bourgain P. The inhibition of prothrombin activation by a lipoïd extract of beef brain. Arch. int. Physiol. Biol. 67: 100 1959;
22 Bourgain R. Etude des propriétés anticoagulantes d’un extrait lipoïdique de cerveau de boeuf. Ed. Acta Medica Belgica, Bruxelles. 1962
23 Bourgain R, Kahn K. Etude de l’antagonisme: extrait lipoïdique de cerveau de boeuf/ thromboplastine tissulaire. Arch. int. Physiol. 69: 390 1961;
24 Kahn M, Bourgain R. Observations supplémentaires sur l’antagonisme extrait lipoïdique de cerveau de boeuf/thromboplastine plasmatique. Arch. int. Pharmacodyn. 138: 345 1962;
25 Folch J. J. Brain cephalin, a mixture of phosphatides : Separation from it of phosphatidylserin, phosphatidyl-etanolamin and a fraction containing an inositol phosphatide. Biol. Chem. 146: 35 1946;
26 Folch J. J. The Structure of Phosphatidylserine. J. Biol. Chem. 174: 439 1948;
27 Wagner H, Horhammer L, Wolff P. Dünnsichtchromatographie von Phosphatiden und Glycolipiden. Biochem. Z. 334: 175 1961;
28 Hanahan D. J, Olley J. N. Chemical nature of monophosphoinositide. J. Biol. Chem. 231: 813 1958;
29 Bereblum I, Chain E. An improved method for the colorimetric determination of phosphate. Biol. J. 32: 295 1938;
30 Hübscher G, Hawthorne J. N. The isolation of inositolmonophosphate from liver. Biol. J. 67: 523 1957;
31 Dole V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J. clin. Invest. 35: 150 1956;