Urokinase Excretion in Man: A Method of Determination and Consideration of its Significance^[*]

Summary

A simple method is described for the quantitative determination of urokinase, a plasminogen activator, in human urine. Plasminogen activation by urokinase and the measurement of the proteolytic activity of the formed plasmin are separated in two individual steps. The normal urokinase excretion values for males and females in various conditions are discussed. Examples of pathological excretion patterns are also given.

^* Supported by grants-in-aid from the American Heart Association and the Belle Bonfils Memorial Blood Bank, Denver.

• Reference

• 1 Albrechtsen O. K. Fibrinolytic activity in the organism. Acta physiol, scand. 47. suppl. 165 1959
• 2 Alkjaersig N, Fletcher P. A, Sherry S. A kinetic study of activation with trypsin, urokinase and streptokinase. J. biol. Chem 233: 86 1958;
• 3 Astrup T, Sterndorff I. An activator of plasminogen in normal urine. Proc. Soc. exp. Biol. (N. Y) 81: 675 1952;
• 4 Bjerrehus I. Fibrinolytic activity of urine. Scand. J. clin. Lab. Invest 04: i79 1952;
• 5 Guest M. M. Profibrinolysin, antifibrinolysin, fibrinogen and urine fibrinolytic factors in the human subject. J. clin. Invest 33: 1553 1954;
• 6 Kaulla K. N. v. Methods for preparation of purified human thromboplastin and fibrinolysin from urine. Acta haemat. (Basel) 16: 315 1956;
• 7 Kaulla K. N. v. Autoaktivierung und Autofibrinolyse. Eine einfache Schnellmethode zur Gewinnung hochaktiven menschlichen Materials. Klin. Wschr 35: 667 1957;
• 8 Kaulla K. N. v. Intravenous protein-free pyrogen, a powerful fibrinolytic agent in man. Circulation 17: 187 1958;
• 9 Kaulla K. N. v. Components of the human fibrinolytic system in blood and urine and their relationship to therapeutic and spontaneous fibrinolysis. In: Connective Tissue, Thrombosis and Atherosclerosis. Edited by Page I. H. Academic Press; New York: 1959
• 10 Kaulla K. N. v, Schneeberger E, Curry M. Quantitative determination of urokinase. Fed. Proc 17: 167 1958;
• 11 Kaulla K. N. v, Swan H. Clotting deviations in man during cardiac bypass: Fibrinolysis and circulating anticoagulant. J. thorac. Surg 36: 519 1958;
• 12 Kaulla K. N. v, Swan H, Paton B. Variations of the thrombin time in certain patients undergoing open-heart surgery. Discussion of its prognostic significance. J. thorac. vase. Surg 40: 260 1960;
• 13 Kaulla K. N. v, Taylor C. A simple test tube method for the production of all-human fibrinolytic material. Fed. Proc 16: 342 1957;
• 14 Kickhoefen B, Struwe F. W, Bramesfeld B, Westphal O. Über einige Beobachtungen am Uropepsinogen und am Plasminogen-Aktivator des menschlichen Urines. Biochem. Z 330: 467 1958;
• 15 Merz W. R. Die Behandlung der Thrombose und Lungenembolie mit Antikoagulantien. Gynaecologia (Basel). suppl. ad vol. 130 1950
• 16 Seelich F, Pantlitschko M, Kaiser E. Zur Frage der fibrinolytischen Wirkung des Harnes: Hemmung der Harnfibrinolyse nach subkutaner Injektion von Hyaluronidase. Enzymologia 16: 56 1953;
• 17 Smyrniotis E. F. Urokinase excretion in man and animals. Fed. Proc 17: 152 1958;
• 18 Smyrniotis E. F, Fletcher P. A, Alkjaersig N, Sherry S. Urokinase excretion in health and its alterations in certain disease states. Thromb. Diath. haem 03: 257 1959;
• 19 Sobel G. W, Mohler S. R, Jones N. W, Dowdy A. B. D, Guest M. M. Urokinase, an activator of plasma fibrinolysin extracted from urine. Amer. J. Physiol 171: 768 1952;
• 20 Williams J. R. B. The fibrinolytic activity in urine. Brit. J. exp. Path 32: 530 1951;