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Thromb Haemost 1961; 05(03): 456-462
DOI: 10.1055/s-0038-1654938
Originalarbeiten – Original Article – Travaux Originaux
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Purified Platelet Cofactor I and Purified Autoprothrombin II in Thromboplastin Generation Test[*]

Orhan N. Ulutin
1   Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA
,
Eberhard F. Mammen
1   Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA
,
Walter H. Seegers
1   Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA
› Author Affiliations
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Publication Date:
21 June 2018 (online)

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Summary

Purified bovine platelet cofactor I substituted for adsorbed plasma in the thromboplastin generation test when Ac-globulin was also supplied. Only 70 gamma/ml were needed. Purified bovine autoprothrombin II substituted for serum in the test. Only 9 gamma were equivalent to serum. Platelet cofactor I thus takes the place of the antihemophilic factor and autoprothrombin II substitutes for PTC and Stuart-Prower factor. We regard the autoprothrombin II preparation used as a single component derived from prothrombin.

* This investigation was supported by a research grant H-2026 from the National Heart Institute, National Institutes of Health, U. S. Public Health Service.


 

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