Separation of Human Fibrinogen and Plasminogen by means of Gel Filtration

Summary

A simple method for removing contaminating plasminogen from fibrinogen is described. The method is based on gel filtration with Sephadex G-200.

When purified fibrinogen was clotted in the presence of urokinase, no lysis was observed during an observation period of 4 weeks.

• References

• Bergström K, Wallen P. Removal of contaminating plasminogen from purified bovine fibrinogen. Arkiv Kemi. 17: 503 1961;
• Blombäck B, Blombäck M. Purification of human and bovine fibrinogen. Arkiv Kemi. 10: 415 1956;
• Blombäck B. On the properties of fibrinogen and fibrin. Arkiv Kemi. 12: 99 1958;
• Celander D. R, Guest M. M. Biochemistry and physiology of urokinase. Amer. J. Cardiol. 06: 415 1960;
• Korsan-Bengtsen K, Ygge J. Purification of human thrombin on a carboxymethylcellulose column. Scand. J. clin. Lab. Invest 13: 591 1961;
• Milstone H. Factor in human blood which participates in streptococcal fibrinolysis. J. Immunol. 42: 109 1941;
• Morrison P. Preparation and properties of serum and plasma proteins. XV. Some factors influencing the quantitative determination of fibrinogen..
• Porath J, Flodin P. Gel filtration: A method for desalting and group separation. Nature 181: 1675 1957;