Thrombosis and Haemostasis, Inhaltsverzeichnis Thromb Haemost 1963; 09(02): 387-394DOI: 10.1055/s-0038-1654992 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH The Influence of Inorganic Salts on Plasma Antithrombin Activity[*] Frank C Monkhouse Ph. D. 1 The Department of Physiology, University of Toronto, Toronto, Ontario › Institutsangaben Artikel empfehlen Abstract Artikel einzeln kaufen Summary A study has been made of factors influencing the activities of plasma antithrombin. Optimal antithrombin activity was obtained between pH 7.8 and 8.5. Dialysis against distilled water reduced the antithrombin activity to less than 30 per cent of normal. The antithrombin activity was restored by the addition, directly or by dialysis, of a number of salts. The increased activity in the presence of the salts seemed to be a function of the polyvalent anions rather than of the ionic strength of the medium. The antithrombin activity of alpha- tocopherol phosphate in vitro appears to be a function of the phosphate ion and not of the vitamin itself. Volltext Referenzen References Fiske C. C, Subbarow Y. The colorimetric determination of phosphorus. J. biol. Chem. 66: 375 1925; Gerendas M. Inactivation of thrombin. Nature 157: 837 1946; Glazko A. J, Greenberg D. M. The mechanism of the inhibiting effect of electrolytes and heparin on blood Coagulation. Amer. J. Physiol. 28: 399 1940; Jaques L. B. The reducing properties of fibrinogen. Biochem. J. 37: 344 1943; Kay J. H, Balia G, Hutton S. B, Ochsner A. The prophylaxis of intravascular clotting by the use of alpha tocopherol and calcium. New Orleans med. surg. J. 103: 116 1950; Lowenthal J, Fisher L. M. The inhibition of blood clotting by phosphate esters of vitamin K and E. Canad. J. Biochem. 37: 501 1958; Maracci M, Comera A. Vitamin E and blood coagulation: alpha-tocopherol phosphate. Third Congr. Intern. Vitamin E. 01: 25 1955; Mihalyi E. Observations on thrombin inactivation by human serum. J. gen. Physiol. 37: 139 1953; Monkhouse F. C, France Evelyn, Seegers W. H. Studies on the antithrombin and heparin cofactor activities of a fraction absorbed from plasma by aluminium hydroxide. Circulât. Res. 03: 397 1955; Seegers W. H, Miller K. D, Andrews E. B, Murphy R. C. Fundamental interactions and effect of storage, ether, adsorbants and blood clotting on plasma antithrombin activity. Amer. J. Physiol. 169: 700 1952;
