Further Purification of the Antihaemophilic Factor (AHF)

H. A Veder

doi:10.1055/s-0038-1655659

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1966; 16(03/04): 738-751
DOI: 10.1055/s-0038-1655659

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Further Purification of the Antihaemophilic Factor (AHF)

H. A Veder

¹From the Biochemical Laboratory of the Paediatric Clinic, University of Amsterdam, Binnengasthuis

› Author Affiliations

Abstract

Summary

A description is given of the further purification of AHF by displacement chromatography on such materials as DEAE-Floc and DEAE-Sephadex. The use of a high ion-exchange capacity, which permits the use of relatively high salt concentrations, together with submaximal loading of the columns produced a high degree of purity of the AHF-preparations. The criteria of purity applied, and some properties of the AHF are described.

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References

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