Thromb Haemost 1957; 01(03/04): 413-417
DOI: 10.1055/s-0038-1656187
Originalarbeiten – Original Article – Travaux Originaux
Schattauer GmbH

A simple method for the preparation of labile factor deficient plasma

Ricardo Katz M.D.
1   Department of Medicine (Dr. H. Alessandri), University of Chile Medical School, Hospital del Salvador, Santiago, Chile
,
Héctor Ducci M.D.
1   Department of Medicine (Dr. H. Alessandri), University of Chile Medical School, Hospital del Salvador, Santiago, Chile
› Author Affiliations
Further Information

Publication History

Publication Date:
08 June 2018 (online)

 

Summary and conclusions

A method is presented which permits the easy preparation of a stable reagent for labile factor determination.

The reagent is obtained in 48 hours by incubation of normal oxalated human plasma in definite amounts and specified containers and further storage in the refrigerator.

This labile factor-deficient plasma behaves similarly to the plasma stored at 4° C for 15 to 25 days.

This method simplifies the routine performance of labile factor determinations.


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  • Bibliography

  • 1 Quick A. J. On the constitution of prothrombin. Am. J. Physiol. 146: 212 1943;
  • 2 Owren P. A. The coagulation of blood. Investigations on a new clotting factor Gundersen; Oslo: 1947
  • 3 Ware A. G, Guest M. M, and Seegers W. H. A factor in plasma which accelerates the activation of prothrombin. J. Biol. Chem. 169: 231 1947;
  • 4 Honorato R. The plasmatic cofactor of thromboplastin: its absorption, with prothrombin and fibrinogen, by alumina and tricalcium phosphate gels. Am. J. Physiol. 150: 381 1947;
  • 5 Owren P. A. Prothrombin and accesory factors; clinical significance. Am. J. Med. 14: 201 1953;
  • 6 Quick A. J, and Stefanini M. Effect of Dicumarol on concentration of the labile factor. Proc. Soc. Exp. Biol. Med. 72: 232 1949;
  • 7 Owren P. A, and Aas K. The control of Dicumarol therapy and the quantitative determination of prothrombin and proconvertin. Scand. J. Clin. Lab. Invest. 3: 201 1951;
  • 8 Stefanini M. Activity of plasma labile factor in disease. Lancet 1: 606 1951;
  • 9 Sykes Jr. E. M, Seegers W. H, and Ware A. G. Effect of acute liver damage on Ac-globulin activity of plasma. Proc. Soc. Exp. Biol. Med. 67: 506 1948;
  • 10 Owren P. A. Parahemofilia: hemorrhagic diathesis, due to absence of a previously unknown clotting factor. Lancet 1: 446 1947;
  • 11 Quick A. J, and Stefanini M. The concentration of the labile factor of the prothrombin complex in human, dog and rabbit blood; its significance in the determination of prothrombin activity. J. Lab. Clin. Med. 33: 819 1948;
  • 12 Wolf P. A modification for routine laboratory use of Stefanini’s method of estimating factor V activity in human oxaiated plasma. J. Clin. Path. 6: 34 1953;
  • 13 Beaumont J. L, and Bernard J. Syndrome hémorrhagique congenital dû au défaut du facteur de coagulation récemment isolé sous le nom de Facteur VII, Convertine, S.P.C.A. (Hypoconvertinemie congénitale hémorragipare). Acta Med. Scand. 145: 198 1953;
  • 14 Vetter H, and Vinazzer H. Proaccelerin deficiency following irradiation with therapeutic doses of radiophosphorus. Blood 9: 163 1954;
  • 15 Quick A. J. The physiology and pathology of hemostasis. Lea & Febiger; Philadelphia: 1951: 120.

  • Bibliography

  • 1 Quick A. J. On the constitution of prothrombin. Am. J. Physiol. 146: 212 1943;
  • 2 Owren P. A. The coagulation of blood. Investigations on a new clotting factor Gundersen; Oslo: 1947
  • 3 Ware A. G, Guest M. M, and Seegers W. H. A factor in plasma which accelerates the activation of prothrombin. J. Biol. Chem. 169: 231 1947;
  • 4 Honorato R. The plasmatic cofactor of thromboplastin: its absorption, with prothrombin and fibrinogen, by alumina and tricalcium phosphate gels. Am. J. Physiol. 150: 381 1947;
  • 5 Owren P. A. Prothrombin and accesory factors; clinical significance. Am. J. Med. 14: 201 1953;
  • 6 Quick A. J, and Stefanini M. Effect of Dicumarol on concentration of the labile factor. Proc. Soc. Exp. Biol. Med. 72: 232 1949;
  • 7 Owren P. A, and Aas K. The control of Dicumarol therapy and the quantitative determination of prothrombin and proconvertin. Scand. J. Clin. Lab. Invest. 3: 201 1951;
  • 8 Stefanini M. Activity of plasma labile factor in disease. Lancet 1: 606 1951;
  • 9 Sykes Jr. E. M, Seegers W. H, and Ware A. G. Effect of acute liver damage on Ac-globulin activity of plasma. Proc. Soc. Exp. Biol. Med. 67: 506 1948;
  • 10 Owren P. A. Parahemofilia: hemorrhagic diathesis, due to absence of a previously unknown clotting factor. Lancet 1: 446 1947;
  • 11 Quick A. J, and Stefanini M. The concentration of the labile factor of the prothrombin complex in human, dog and rabbit blood; its significance in the determination of prothrombin activity. J. Lab. Clin. Med. 33: 819 1948;
  • 12 Wolf P. A modification for routine laboratory use of Stefanini’s method of estimating factor V activity in human oxaiated plasma. J. Clin. Path. 6: 34 1953;
  • 13 Beaumont J. L, and Bernard J. Syndrome hémorrhagique congenital dû au défaut du facteur de coagulation récemment isolé sous le nom de Facteur VII, Convertine, S.P.C.A. (Hypoconvertinemie congénitale hémorragipare). Acta Med. Scand. 145: 198 1953;
  • 14 Vetter H, and Vinazzer H. Proaccelerin deficiency following irradiation with therapeutic doses of radiophosphorus. Blood 9: 163 1954;
  • 15 Quick A. J. The physiology and pathology of hemostasis. Lea & Febiger; Philadelphia: 1951: 120.