Introduction: Primary graft dysfunction (PGD) is the main cause of early mortality after lung transplantation
(LTx). Multiple mechanisms have been described, but the link between PGD, T cell priming
and immune answer has not been defined. In humanized mice, we studied leukocytes from
human lung recipients undergoing PGD with different kinetics and their impact on rejection
of artery grafts.
Methods: Segments of the pericardiophrenic arteries were procured from surplus donor lung
tissue and implanted into the abdominal aorta of immunodeficient mice. Development
of transplant arteriosclerosis after reconstitution with PBMC was determined at day
28. Experiments were retrospectively divided; six patients developed transient PGD
grade ≥2 at T0. Five patients developed PGD grade ≥2 at T24 and/or T48.
Mice from groups A–C were reconstituted with PBMC from PGD+ T0 patients. Group A mice
were reconstituted with PBMC from the respective allogeneic human recipient. Group
B mice additionally received CD4+CD25high Treg cells with their PBMC. In Group C, administered PBMC were depleted of CD4+CD25high Treg cells. Mice from groups D-F were reconstituted with PBMC from PGD+ T24/T48 patients,
with group D receiving unmodified PBMC, group E receiving Treg enriched PBMC and group
F receiving PBMC depleted of Tregs.
Results: Luminal occlusion of aortic vessels was higher in group D mice compared to group
A. The addition of CD4+CD25high Treg cells in group B & E mice had a suppressive effect on luminal occlusion.
Conclusions: We conclude that leukocytes from lung transplantation recipients undergoing PGD 2
or 3 transfer enhanced alloreactivity to humanized mice already immediately perioperatively.
Therein, PGD 2 or 3 at T24 and/or T48 hours after lung transplantation translates
to more severe rejection in humanized mice than transient PGD 2 or 3 at T0 only. This
inflammatory response is suppressed by CD4+CD25high Treg cells. This could be a potential target for future interventions in lung transplantation.
