CC BY-NC-ND 4.0 · Laryngorhinootologie 2019; 98(S 02): S66
DOI: 10.1055/s-0039-1685966
Abstracts
Oncology

Immunomodulatory Effects Of Cisplatin On hMSC in vitro

S Bischoff
1   Universitätsklinkum Würzburg, Würzburg
,
S Hackenberg
1   Universitätsklinkum Würzburg, Würzburg
,
A Scherzad
1   Universitätsklinkum Würzburg, Würzburg
,
R Hagen
1   Universitätsklinkum Würzburg, Würzburg
,
T Gehrke
1   Universitätsklinkum Würzburg, Würzburg
› Author Affiliations
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    Introduction:

    The use of cytostatic drugs like Cisplatin is a common therapeutic strategy for head and neck carcinoma. The tumor microenvironment, including human mesenchymal stem cells (hMSC), is crucial for carcinogenesis. Besides its cytotoxicity, Cisplatin in subtoxic concentrations shows immunomodulatory effects. The objective of this study was to investigate the immunomodulatory impact of Cisplatin on hMSC regarding the expression of cytokines and growth factors in vitro.

    Methods:

    hMSCs of bone marrow tissue were used. Cells were incubated with Cisplatin in subtoxic concentrations for 24 hours and further cultivated for 72 hours. Cells in the control group were cultivated without Cisplatin. After the cultivation, PGE2, TGF-ß, IL-6 und Ido, which are known to be important factors for the immunomodulation, were quantified by ELISA. To avoid toxic effects of Cisplatin on hMSC, cell proliferation was measured by FACS and scratch-assays.

    Results:

    A significant increase of PGE2, TGF-ß, IL-6 und Ido could be shown after incubation with low-dosed Cisplatin compared to the control group. Toxic effects of Cisplatin to hMSC could neither be shown regarding proliferation, migration nor viabililty.

    Conclusion:

    Cisplatin leads even in non-cytotoxic concentration to an increased secretion of immunomodulatory cytokines in hMSC. In future studies the immunomodulatory effects of Cisplatin, especially in co-culture with lymphocytes as effectorcells of the cytokines will be analysed to evaluate new strategies for cancer treatment.


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    Stefanie Bischoff
    Universitätsklinkum Würzburg,
    Klinik für Hals-, Nasen- und Ohrenkrankheiten, Joseph-Schneider-Str. 11, 97080
    Würzburg

    Publication History

    Publication Date:
    23 April 2019 (online)

    © 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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