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DOI: 10.1055/s-0039-1686096
Theranostic UV activatable TiO2 nanoparticles as catalyst for selective tumor cell apoptosis
Metal oxide nanoparticles (NP) like titanium dioxide (TiO2) are taken up by cells via endocytosis within a few minutes after exposition. Endocytosis of NP is known to be higher in malignant cells compared to benign cells. Preliminary work showed that functionality of TiO2-NP mainly depends on their physical properties. Our aim was to examine the potential of theranostic TiO2-NP in human cells.
Fluorescence-labelled TiO2-NP were dispersed in the organic acid FIW1 and pre-activated by UVC light. Polycarboxylate ether served as an inert stabilizer for the NP dispersion. FaDu, HLaC78 and non-malignant cells (fibroblasts and MSC) were then exposed to the dispersion for 24h. Besides physicochemical characterization, the MTT assay was performed for toxicological analysis. Cells incubated with non-photoactivated TiO2-NP served as control.
Fluorescent TiO2-NP were preferably taken up by FaDu and HLaC78 cells compared to fibroblasts and MSC. Only after UV pre-activation the NP dispersion showed a high toxicity in tumor cells which was not achieved in fibroblasts and MSC even at high doses. Without UV activation, the dispersion was non-toxic. NP-free medium supernatant showed certain antitumoral effects as well.
Inactive fluorescence-labelled TiO2-NP are non-toxic and selectively taken up by tumor cells via endocytosis. TiO2-NP act as photocatalysts for the synthesis of a tumor-toxic substance derived from FIW1 acid. NP themselves represent herein the catalyst without intrinsic toxicity. The combination of fluorescence-labelling and catalytic potential make TiO2-NP a promising theranostic agent.
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Publikationsverlauf
Publikationsdatum:
23. April 2019 (online)
© 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
Georg Thieme Verlag KG
Stuttgart · New York