CC BY-NC-ND 4.0 · Laryngorhinootologie 2019; 98(S 02): S88
DOI: 10.1055/s-0039-1686103
Abstracts
Oncology

Proton pump (H+/K+-ATPase) expression in normal laryngeal tissue and laryngeal carcinoma-The role of proton pump in laryngeal tissues

S Zhou
1   The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
,
YJ Ao
1   The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
,
KJ Cheng
1   The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
,
YY Bao
1   The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
,
LF Shen
1   The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
,
J Fan
1   The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
› Author Affiliations
 
 

    Background/Aims:

    The proton pump (H+/K+-ATPase) is primarily located in the parietal cells of the stomach. The proton pump (H+/K+-ATPase) was also detected outside the stomach. Some studies also found H+/K+-ATPase distributed in the human larynx and suggested that acid may be produced not only by the gastric parietal cells but also by H+/K+-ATPase cells in the human larynx. Several studies have suggested that GERD or LPRD is an independent risk for laryngeal carcinoma. However, whether H+/K+ ATPase is involved in this process has not been investigated.

    Methods:

    We detected the gastric proton pump (H+/K+-ATPase) α- and β-subunits in normal laryngeal tissues and investigated the distribution in the larynx by real-time RT-PCR and Western blotting. We also studied expression of the proton pump (H+/K+-ATPase) α- and β-subunits in laryngeal carcinoma and analyzed the relationship between expression of the α- and β-subunits and laryngeal carcinoma. We found the expression levels of α-subunit and β-subunit mRNA in normal epiglottic mucosa, ventricular fold mucosa, arytenoid mucosa, epiglottic cartilage, and vocal fold mucosa. Expression of (H+/K+-ATPase), either the α- or β-subunit, was significantly higher in carcinoma tissues than that in paracarcinoma tissues (p < 0.001, p < 0.001, respectively) and normal laryngeal tissues (p < 0.001, p < 0.001, respectively). Survival was not significantly associated with any clinicopathological parameter, including α-and β-subunit expression of (H+/K+-ATPase).

    Conclusion:

    H+/K+-ATPase exists in larynx and altered expression of H+/K+-ATPase may be involved in the process of laryngeal carcinogenesis.


    #
    Professor, MD, PhD Shuihong Zhou
    The First Affiliated Hospital, College of Medicine, Zhejiang University,
    79 Qingchun Road, 310003
    Hangzhou, China

    Publication History

    Publication Date:
    23 April 2019 (online)

    © 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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