CC BY-NC-ND 4.0 · Laryngorhinootologie 2019; 98(S 02): S129
DOI: 10.1055/s-0039-1686380
Abstracts
Otology

Comparing the effect of surgically plugging the superior semicircular canal with different materials on tissue formation and pathology in the middle ear of gerbils

O Gleich
1   HNO-Klinik Universität Regensburg, Regensburg
,
P Kwok
1   HNO-Klinik Universität Regensburg, Regensburg
,
J Strutz
1   HNO-Klinik Universität Regensburg, Regensburg
,
C Bohr
1   HNO-Klinik Universität Regensburg, Regensburg
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    Introduction:

    Plugging is an established therapy to treat the symptoms caused by a dehiscence of the superior semicircular canal. We used the gerbil as a model to evaluate the success, the degree of tissue formation and histological signs of pathology in the middle ear after plugging the canal with different materials.

    Methods:

    The canal was surgically opened in the dorsal mastoid cavity and plugged with Teflon, bone wax, bone or fat/muscle. Approximately 8 weeks after surgery the ears were harvested for processing, fixed and decalcified. The degree of tissue formation in the middle ear around the surgical opening of the canal was documented and subsequently the ears were embedded in paraffin to histologically evaluate the newly formed tissue in 10 µm thick HE stained sections.

    Results:

    A complete block of the canal was verified histologically in all ears after Teflon (N = 11), bone wax (N = 12) and bone (N = 13) while only 5 of 14 ears showed a successful occlusion after using the soft tissues fat/muscle.

    Following bone wax, the degree of newly formed tissue in the middle ear in the region of the operated canal was significantly higher compared to Teflon and bone. In addition, 8 weeks after surgery, the newly formed tissue showed a higher degree of secondary osteonecrosis after plugging the canal with bone wax.

    Conclusions:

    Plugging of the superior semicircular canal after surgical opening was 100% successful using Teflon, bone wax or bone in the gerbil model. Due to the higher degree of tissue formation and the increased incidence of secondary osteonecrosis in the newly formed bone after bone wax, Teflon and bone appear advantageous for plugging the superior semicircular canal.


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    Prof. Dr. rer. nat. Otto Gleich
    HNO-Klinik Universität Regensburg,
    Franz-Josef-Strauß-Allee 11, 93042
    Regensburg

    Publikationsverlauf

    Publikationsdatum:
    23. April 2019 (online)

    © 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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