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J Pediatr Genet 2019; 08(04): 193-197
DOI: 10.1055/s-0039-1696976
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Genetic Landscape of Mitochondrial Regulatory Region in Pediatric Acute Myeloid Leukemia: Changes from Diagnosis to Relapse

Anudishi Tyagi
1   Department of Medical Oncology, Dr. B. R. A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India
,
Raja Pramanik
1   Department of Medical Oncology, Dr. B. R. A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India
,
Radhika Bakhshi
2   Department of Biomedical Science, Shaheed Rajguru College of Applied Sciences, University of Delhi, New Delhi, India
,
Sreenivas Vishnubhatla
3   Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India
,
Sameer Bakhshi
1   Department of Medical Oncology, Dr. B. R. A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India
› Author Affiliations Funding We acknowledge the following funding sources: Department of Biotechnology, Government of India, under grant number BT/PR7197/MED/30/899/2012 (September 26, 2013) and AIIMS, Institutional Junior Research Fellowship grant.
Further Information

Publication History

16 May 2019

05 August 2019

Publication Date:
12 September 2019 (online)

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Abstract

This prospective study aimed to compare the pattern of mitochondrial deoxyribonucleic acid D-loop (mt-DNA D-loop) variations in 41 paired samples of de novo pediatric acute myeloid leukemia (AML) (baseline vs. relapse) patients by Sanger's sequencing. Mean mt-DNA D-loop variation was 10.1 at baseline as compared with 9.4 per patients at relapse. In our study, 28 (68.3%) patients showed change in number of variations from baseline to relapse, 11 (26.8%) patients showed increase, 17 (41.6%) patients showed decrease, and 7 (17.1%) patients who suffered a relapse had a gain at position T489C. No statistically significant difference was observed in the mutation profile of mt-DNA D-loop region from baseline to relapse in the evaluated population of pediatric AML.

Keywords

acute myeloid leukemia - mitochondrial DNA - D-loop - variations - relapse
 

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