Planta Med 2019; 85(18): 1506
DOI: 10.1055/s-0039-3399939
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Unprecedented isoflavanoid-neoflavonoid analogues isolated from Brazilian red propolis

JR Gubiani
1   Instituto de Química de São Carlos, Universidade de São Paulo,, CP 780, CEP 13560-970, São Carlos, SP, Brazil
,
TP Banzato
2   Department of Structural and Functional Biology, Institute of Biology, University of Campinas,, Campinas, SP, Brazil
3   Chemical, Biological and Agricultural Pluridisciplinary Research Center, University of Campinas,, Campinas, SP, Brazil
,
CR Nogueira
4   Faculty of Science and Technology, Federal University of Grande Dourados,, 79804-970, Dourados, MS Brazil
,
AF Monteiro
1   Instituto de Química de São Carlos, Universidade de São Paulo,, CP 780, CEP 13560-970, São Carlos, SP, Brazil
,
SM de Alencar
5   Department of Agri-food Industry, Food and Nutrition, University of São Paulo,, Piracicaba, SP, Brazil
,
RA Pilli
6   Instituto de Química, Universidade Estadual de Campinas,, Campinas, SP, Brazil
,
JE de Carvalho
2   Department of Structural and Functional Biology, Institute of Biology, University of Campinas,, Campinas, SP, Brazil
7   Faculty of Pharmaceutical Sciences, University of Campinas,, Campinas, SP, Brazil
,
RGS Berlincka
1   Instituto de Química de São Carlos, Universidade de São Paulo,, CP 780, CEP 13560-970, São Carlos, SP, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Propolis is a natural resinous product collected by honeybees from the buds and exudates of various plant sources, which has been used as a traditional remedy in folk medicine for centuries. Among Brazilian propolis varieties, Brazilian red propolis (BRP) is isoflavonoid-rich. Bees producing red propolis feed upon Dalbergia ecastophyllum [1]–[4] In this investigation, BRP was extracted with 80% EtOH shaken at 70°C for 30 min. After extraction, the mixture was centrifuged. The supernatant was evaporated to dryness to give a EtOH extract of Brazilian red propolis (EERP) [1],[2]

The EERP extract was fractionated by normal phase silica gel column eluted with a linear gradient of CHCl3/EtOAc. Fraction 5 was separated by C18 RPCC and eluted with a linear gradient of MeOH in H2O. Fractions collected were evaporated to dryness, resulting in thirty fractions. After successive fractionations, eight structurally novel isoflavanoid-neoflavonoid analogues were obtained and identified by extensive analysis of spectroscopic data. The cytotoxic and anti-inflammatory activities of isoflavanoid-neoflavonoid analogues are under evaluation.


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Acknowledgements

FAPESP (Fundação de Amparo `a Pesquisa do Estado de São Paulo, grant #2013/50228-8) JRG also thanks FAPESP (2017/06014-4) for fellowship.

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