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DOI: 10.1055/s-0039-3399957
Anti-inflammatory activity and ROS regulation of sinapaldehyde in LPS-stimulated RAW 264.7 macrophages
Publication History
Publication Date:
20 December 2019 (online)
Sinapaldehyde is a member of the class of compounds known as methoxyphenols. Sinapaldehyde can be found in plant extracts such as Senra incana and Ailanthus altissima Swingle [1],[2]. This study evaluated the anti-inflammatory effect of sinapaldehyde in LPS-stimulated RAW 264.7 macrophages using nitric oxide (NO) assay, cytokine ELISA, and reverse transcription polymerase chain reaction analyses. Sinapaldehyde exhibited inhibitory activity on the production of NO, ROS, and cytokines such as TNF-α and IL-6. Sinapaldehyde (100 μM) significantly inhibited total NO and ROS inhibitory activity at 93% (p < 0.001) and 34% (p < 0.05), respectively, when compared with LPS control. Moreover, sinapaldehyde significantly (p < 0.001) down-regulated the mRNA expression of inflammatory marker genes like TNF-α, IL-6, and iNOS against LPS stimulation. Sinapaldehyde did not show any cytotoxicity at different concentrations (3 - 100 µM). Our results demonstrate that sinapaldehyde is able to show anti-inflammatory effects by suppression of ROS and NO. It could be used as a new natural product for the effective treatment of inflammatory diseases.
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References
- 1 Farah MH, Samuelsson G. Pharmacologically active phenylpropanoids from Senra incana. Planta Med 1992; 58: 14-18
- 2 Kim HM, Lee JS, Sezirahiga J, Kwon J, Jeong M, Lee D, Choi JH, Jang DS. A New Canthinone-Type Alkaloid Isolated from Ailanthus altissima Swingle. Molecules 2016; 21: E642
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References
- 1 Farah MH, Samuelsson G. Pharmacologically active phenylpropanoids from Senra incana. Planta Med 1992; 58: 14-18
- 2 Kim HM, Lee JS, Sezirahiga J, Kwon J, Jeong M, Lee D, Choi JH, Jang DS. A New Canthinone-Type Alkaloid Isolated from Ailanthus altissima Swingle. Molecules 2016; 21: E642