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DOI: 10.1055/s-0039-3403103
Surface proteome of plasma extracellular vesicles as biomarkers for pneumonia and acute exacerbation of COPD
Publication History
Publication Date:
28 February 2020 (online)
Community-acquired pneumonia (CAP) and acute exacerbations of chronic obstructive pulmonary disease (AECOPD) represent a major burden of morbidity and mortality and their differential diagnosis is critical. A potential source of relevant accessible biomarkers are blood-borne small extracellular vesicles (sEVs).
We performed an EV Array to find proteins on plasma sEVs that are differentially expressed and possibly allow the differential diagnosis between CAP and AECOPD. Plasma samples from 21 healthy subjects, 24 CAP and 10 AECOPD patients were analyzed. The EV Array contained 40 antibodies to capture sEVs, which were then visualized with a cocktail of biotin-conjugated CD9, CD63 and CD81 antibodies.
We detected significant differences in the protein decoration of sEVs between healthy subjects and CAP or AECOPD patients. We found CD45 and CD28 to be the best discrimination markers between CAP and AECOPD in ROC analyses with an area under the curve of > 0.92. Additional ensemble feature selection revealed the possibility to distinguish between CAP and AECOPD even if the CAP patient had COPD, with a panel of CD45, CD28, CTLA4, TNF-R-II.
The discrimination of sEV-associated proteins is a minimally-invasive method with potential to discriminate between CAP and AECOPD patients.
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