Proliferation of epithelial tumor cells in presence of human mesenchymal stem cells

The assumed tropism of mesenchymal stem cells (MSC) towards epithelial tumor cells is the basis for the exploration of new tumor therapy strategies. So far, no clear behavioral patterns of MSC have been reported in the presence of tumor cells. On the one hand, MSC were shown to have a high-proliferative growth in low-density seeding. On the other hand, the surface marker Stro-1 appears to be stem cell-specific and to influence the behavior of MSC. The aim of this study was to investigate the proliferation behavior of the human hypopharyngeal carcinoma cell line FaDu in the presence of different MSC colonies.

MSC were isolated from the femoral head of 11 patients and cultivated in three experimental approaches (different degrees of density, enrichment of Stro-1 positive MSC and double passaging). These three approaches per stem cell donor were then co-cultured with FaDu and the proliferation was compared with a FaDu monoculture by electronic and microscopic cell counting.

The proliferation of FaDu did not show significant differences between mono- and co-culture with MSC in any of the three approaches. The proliferation of MSC tended to be lower than the proliferation of FaDu. The generation of highly proliferative clones of thinly seeded MSC could not be confirmed in general, the yield of Stro-1 positive MSC was low.

The Results confirm the inconsistencies reported in the literature with regard to the properties of MSC and their influence on epithelial tumor cells. The hypothesis that it is possible to isolate stem cells with specific properties by different cultivation Methods or individual surface markers could not be confirmed. A more accurate molecular analysis of MSC therefore appears necessary.

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Artikel online veröffentlicht:
10. Juni 2020