CC BY-NC-ND 4.0 · Laryngorhinootologie 2020; 99(S 02): S263
DOI: 10.1055/s-0040-1711107
Abstracts
Otology

Bacterial biofilms on cochlear implants – visualization of an altered morphology after the application of bioactive glass (BAG)

B Höing
1   Klinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Essen, Universität Duisburg-Essen Essen
,
L Kirchhoff
2   Institut für medizinische Mikrobiologie, Universitätsklinikum Essen, Universität Duisburg-Essen Essen
,
J Arnolds
1   Klinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Essen, Universität Duisburg-Essen Essen
,
S Hansen
1   Klinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Essen, Universität Duisburg-Essen Essen
,
T Hussain
1   Klinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Essen, Universität Duisburg-Essen Essen
,
S Lang
1   Klinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Essen, Universität Duisburg-Essen Essen
,
Jörg Steinmann
3   Institut für Klinikhygiene, Medizinische Mikrobiologie und Klinische Infektiologie, Universitätsklinik der Paracelsus Medizinischen Privatuniversität Nürnberg Nürnberg
,
D Arweiler-Harbeck
1   Klinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Essen, Universität Duisburg-Essen Essen
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    Introduction Bacterial biofilm formation on medical devices, such as cochlear implants (CI), can lead to chronic infections. Interestingly, bioactive glass (BAG) of type S53P4 seems to be a promising tool for use in the reduction of biofilm development. The aim of this investigation was to visualize changes in biofilm morphology via scanning electron microscopy (SEM) before and after the application of bioactive glass.

    Methods Primarily, four bacterial species known to cause implant-related infections, P. aeruginosa (ATCC9027), S. aureus (ATCC6538), Staphylococcus epidermidis (ATCC12228) and Streptococcus pyogenes (ATCC19615) were analyzed regarding their capacity to form biofilm on CI components manufactured from three kinds of material: silicone, platinum and titanium. Subsequently, P. aeruginosa and S. aureus biofilms were visualized using scanning electron microscopy, comparing BAG-treated biofilm with non-treated biofilm.

    Results The four bacterial species presented biofilm-forming capabilities in a species and surface dependent manner. S. aureus and P. aeruginosa showed the highest rate of biofilm formation on polystyrene surfaces. For both species, SEM revealed altered biofilm morphology after treatment of S53P4 BAG.

    Conclusion This study indicates that bacterial biofilm formation and structure on CI components is dependent on the surface composition. After application of BAG, changes in biofilm morphology on CI components were observed. These data highlight the impact of BAG on bacterial biofilm morphology.


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    Advanced Bionics, Cochlear, MedEl, BonAlive

    Höing Benedikt
    Klinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum Essen, Universität Duisburg-Essen
    Hufelandstraße 55
    45147 Essen

    Publikationsverlauf

    Artikel online veröffentlicht:
    10. Juni 2020

    © 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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