Objective Although the value of serum Anti-Mullerian hormone (AMH) is well known, the influence
of AMH on the follicular level has not yet been sufficiently clarified. The aim of
this study was to investigate how AMH behaves in terms of follicle size, the presence
of an oocyte, sociodemographic parameters and other hormones and vitamins.
Material/Methods We investigated the follicular fluid AMH (fAMH) and serum AMH (sAMH) of 61 female
fertility patients undergoing IVF or ICSI therapy (fAMH n= 376, sAMH n=44). On the
day of oocyte retrieval, we collected the follicular fluid (FF) of each follicle separately
and also a serum sample. The samples were analyzed regarding their AMH level and other
hormone concentrations. The follicles were divided into groups (large≥16mm, small<
16mm) and the presence of oocytes.
Results Follicles with a large diameter contained a significantly lower concentration of
fAMH (p=0.001). Follicles that contain an oocyte showed a significantly higher fAMH
level than those without one (p< 0.001). We also showed that there is a significantly
but weak correlation between fAMH and sociodemographic parameters such as the patients
age (r=-0.11, p< 0.001) and BMI (r=0.03, p< 0.006). The same applies to the connection
between fAMH and other parameters such as Vitamin D (r=-0.13, p< 0.001), LH (r=-0.35,
p< 0.001) and progesterone (r=-0.21, p< 0.001) in FF.
Conclusion Our results confirm that larger follicles don´t produce more fAMH than smaller ones.
We were able to demonstrate that the presence of an oocyte is significantly associated
with the level of fAMH.
