CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S103
DOI: 10.1055/s-0041-1727900
Abstracts
Head-Neck-Oncology

Establishment and characterisation of patient-derived head and neck cancer models from surgical specimens and endoscopic biopsies

D Strüder
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie "Otto Körner", Universitätsmedizin Rostock, Rostock
,
T Momper
2   Medizinische Klinik III für Hämatologie, Onkologie und Palliativmedizin, Universitätsmedizin Rostock, Rostock
,
N Irmscher
2   Medizinische Klinik III für Hämatologie, Onkologie und Palliativmedizin, Universitätsmedizin Rostock, Rostock
,
J Liese
3   Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie, Universitätsmedizin Rostock, Rostock
,
S Schraven
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie "Otto Körner", Universitätsmedizin Rostock, Rostock
,
A Zimpfer
4   Institut für Pathologie, Universitätsmedizin Rostock, Rostock
,
B Frerich
3   Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie, Universitätsmedizin Rostock, Rostock
,
C Junghanss
2   Medizinische Klinik III für Hämatologie, Onkologie und Palliativmedizin, Universitätsmedizin Rostock, Rostock
,
R Mlynski
1   Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie "Otto Körner", Universitätsmedizin Rostock, Rostock
,
C Maletzki
2   Medizinische Klinik III für Hämatologie, Onkologie und Palliativmedizin, Universitätsmedizin Rostock, Rostock
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    Head and neck cancer exhibits considerable heterogeneity in biologic behaviour and therapeutic response. Patient-derived xenografts (PDX) maintain morphology and molecular profiling of the original tumours, thus providing a platform for the examination of disease biology and novel therapeutic agents.

    However, restricted availability of tumour samples hindered the widespread use of PDX. Most PDX-projects include only surgical specimens because reliable engraftment from biopsies has not been reported. Sample collection is limited and excludes recurrent and metastatic cancer (that cannot be treated surgically) from preclinical models as well as future personalised medicine.

    This study examines the availability of suitable samples using routine endoscopic biopsies: PDX-take rate, –growth, histopathology and molecular characteristics were compared with regular surgery samples. Specimens from endoscopic biopsies and surgery were analysed for the viable tumour tissue and fragments of 3x3x3 mm3 were implanted into NSG mice (sc). This study shows that engraftment occurs in both biopsies and surgical specimens. However, the engraftment rate was lower for biopsies (25 %  vs. 57 % ) and engraftment took longer (11 vs. 7 weeks). After successful engraftment, growth kinetics were similar. To examine these limits in biopsy engraftment, the study analyses the sample size, time of ischemia, HE histology, proliferation (Ki-67), immune status (PD-L1, CD8/45/56/68/163) and mutational profile (in-house Cancer Hotspot Panel) of the samples and the corresponding PDX. The results may improve PDX preparation to include aggressive carcinomas, which cannot be treated by surgery. Thus, the relation of PDX-engraftment and clinical outcome might lead to the identification of novel clinical biomarkers.

    Poster-PDF A-1560.pdf


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    Conflict of interest

    Der Erstautor gibt keinen Interessenskonflikt an.

    Address for correspondence

    Strüder Daniel
    Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie "Otto Körner", Universitätsmedizin Rostock
    Rostock

    Publication History

    Article published online:
    13 May 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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