CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S113
DOI: 10.1055/s-0041-1727933
Abstracts
Head-Neck-Oncology: Clinical Studies

Clinical application of personalized ddPCR assays in liquid profiling of blood and saliva allows early recurrence detection of head and neck tumors

R Rösch
1   Klinikum rechts der Isar der TU München, Institut für Klinische Chemie und Pathobiochemie, München
,
I Kerle
2   Klinikum rechts der Isar der TU München, Klinik und Poliklinik für Innere Medizin III, Hämatologie und Onkologie, München
,
N Pfarr
3   Technische Universität München, Institut für Pathologie, München
,
K Steiger
3   Technische Universität München, Institut für Pathologie, München
,
F Stögbauer
3   Technische Universität München, Institut für Pathologie, München
,
J Rinecker
4   Klinikum rechts der Isar der TU München, Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde, München
,
S Krippgans
5   Klinikum rechts der Isar der TU München, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, München
,
C Straube
6   Klinikum rechts der Isar der TU München, Klinik und Poliklinik für RadioOnkologie und Strahlentherapie, München
,
M Nieberler
5   Klinikum rechts der Isar der TU München, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, München
,
Jürgen Ruland
1   Klinikum rechts der Isar der TU München, Institut für Klinische Chemie und Pathobiochemie, München
,
B Wollenberg
4   Klinikum rechts der Isar der TU München, Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde, München
,
M Wirth
4   Klinikum rechts der Isar der TU München, Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde, München
,
C Winter
1   Klinikum rechts der Isar der TU München, Institut für Klinische Chemie und Pathobiochemie, München
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    Introduction Liquid profiling could provide improved tumor monitoring in patients (pts.) with head and neck cancer (HNSCC) and improved early recurrence detection. As part of a proof-of-concept study, we designed personalized, tumor-specific ddPCR mutation assays and analyzed their suitability for early recurrence detection by measuring ctDNA in blood and saliva.

    Material and Methods In 8 HNSCC pts. blood and saliva samples were prospectively collected. In 7 pts. we collected pre-operative plasma samples, in 7 pts. additional saliva samples in the postoperative course. Mutations in the primary tumor tissue were analyzed with panel sequencing (45 genes, 224 amplicons). Based on NGS-results, tumor-specific ddPCR-mutation assays were designed and ctDNA was analyzed in plasma and saliva.

    Results On average, 6 blood and 2 saliva samples per patient were examined. Using tumor-specific ddPCR assays, ctDNA was detected in preoperative plasma samples in 71 %  of the pts. (5/7). During the follow-up period, we detected ctDNA in 50 %  (4/8) in plasma and 100 %  (7/7) in saliva samples. 5 of the pts. developed a recurrence, 3 pts. are currently clinically tumor-free. An increase in ctDNA levels was associated with a clinical recurrence in 80 %  (plasma) and/or 100 %  (saliva) of the examined pts. . Using minimally invasive liquid profiling a ctDNA increase was measured in a median of 6 months (1 to 20 mo.) in plasma and 7 months (1 to15 mo.) in saliva before clinical recurrence diagnosis.

    Conclusion CtDNA detection with tumor-specific ddPCR-mutation assays is a promising minimally invasive tool for tumor monitoring. Saliva appears to be well-suited for the early recurrence detection of even small tumors of the oral cavity and oropharynx.

    Poster-PDF A-1626.pdf


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    Conflict of interest

    Der Erstautor gibt keinen Interessenskonflikt an.

    Address for correspondence

    Rösch Romina
    Klinikum rechts der Isar der TU München, Institut für Klinische Chemie und Pathobiochemie
    Ismaninger Str. 22
    81675 München

    Publication History

    Article published online:
    13 May 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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