CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S200
DOI: 10.1055/s-0041-1728371
Abstracts
Otology / Neurotology / Audiology

Reduction of bacterial biofilm on cochlear implants - an experimental in vitro analysis using scanning electron microscopy

B Höing
1   Klinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie, Universitätsklinikum Essen, Essen
,
L Kirchhoff
2   Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Essen
,
J Steinmann
3   Institut für Klinikhygiene, Medizinische Mikrobiologie und Klinische Infektiologie, Universitätsklinik der Paracelsus Medizinischen Privatuniversität Nürnberg, Nürnberg
,
T Hussain
1   Klinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie, Universitätsklinikum Essen, Essen
,
L Holtmann
1   Klinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie, Universitätsklinikum Essen, Essen
,
S Lang
1   Klinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie, Universitätsklinikum Essen, Essen
,
J Buer
2   Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Essen
,
D Arweiler-Harbeck
1   Klinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie, Universitätsklinikum Essen, Essen
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    Bacterial biofilm formation on medical devices, such as cochlear implants (CI), can lead to chronic infections. Interestingly, bioactive glass S53P4 seems to be a promising tool for biofilm reduction on CI components. In this study, biofilm formation before and after application of bioactive glass on different CI components was evaluated and visualized via electron microscopy.

    On first level analysis, four bacterial species causing implant-related infections were evaluated. Pseudomonas aeruginosa (ATCC9027), Staphylococcus aureus (ATCC6538), Staphylococcus epidermidis (ATCC12228) and Streptococcus pyogenes (ATCC19615). On second level, surface design and biofilm formation of S. aureus and its reduction after application of S53P4 bioactive glass were visualized via microscopy, fluorescence microscopy and electron microscopy.

    The four bacterial strains showed an individual biofilm formation preferably on metal CI components. In particular, S. aureus showed the highest biofilm rate on the polystyrene surface. Thereby, A morphologic difference of S. aureus biofilm after application of S53P4 bioactive glass was detected in biofilm visualization via electron microscopy.

    S. aureus is a strong biofilm builder especially on metal CI components. This study revealed an alteration in biofilm morphology on CI components via electron microscopy after application of bioactive glass.

    Poster-PDF A-1232.pdf

    Advanced Bionics, Bon Alive, Cochlear, MED-EL


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    Conflict of interest

    Der Erstautor gibt keinen Interessenskonflikt an.

    Address for correspondence

    Höing Benedikt
    Klinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie, Universitätsklinikum Essen
    Essen

    Publication History

    Article published online:
    13 May 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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