Keywords
SARS-CoV-2 infection - pregnancy - severity of COVID-19 disease - neonatal outcomes
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak was first reported in December 2019 from Wuhan and had an enormous global impact.[1] As of April 2021, more than 140 million cases were infected with SARS-CoV-2 and more than 3 million were died.[2]
A wide range of clinical presentations have been reported with asymptomatic patients at one edge and the catastrophic pneumonia leading to respiratory failure on the other.[3] Elder people and those with comorbidities such as hypertension and diabetes mellitus have worse clinical course.[3] Younger patients and being female in sex are reported to be associated with improved outcome.[4] Pregnant patients with SARS-CoV-2 infection are at higher risk for hospitalization, mechanical ventilation, intensive care unit admission, and preterm delivery.[5] In addition, pregnant women infected with SARS-CoV-2 appear to have higher morbidity and mortality compared with general population.[6]
[7] SARS-CoV-2 infection in pregnancy was found to be associated with preeclampsia, preterm birth, and stillbirth.[8] Although the majority of SARS-CoV-2 infected newborns are asymptomatic or have milder disease, moderate-to-severe symptoms including multiorgan failure and mortality have also been reported in the neonatal population, especially in premature infants.[9]
[10]
Recent data also suggest that pregnant and peripartum women have more severe illness in the second wave of the COVID-19 pandemic than was observed in the first wave.[11] Severe SARS-CoV-2 infection in pregnancy was reported to be associated with preeclampsia, preterm birth, gestational diabetes, and low birth weight compared with mild infection.[8] However, to our best of knowledge, no study primarily focused on the effects of the severity of maternal SARS-CoV-2 infections on neonatal morbidities and mortality.
Therefore, the objective of this study is to evaluate the effect of the severity of maternal SARS-CoV-2 infection on neonatal outcomes.
Materials and Methods
This retrospective study was conducted at Istanbul Basaksehir Cam and Sakura City Hospital between October 2020 and January 2021. The clinical and laboratory data of 40 pregnant women and neonates were evaluated after local ethical committee approval obtained (Istanbul Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Hospital, approval no. 2021–21). All pregnant women with positive real time reverse transcriptase polymerase chain reaction (RT-PCR) test for SARS-CoV-2 were included in the study.
The maternal disease severity was classified as mild, moderate, or severe according to the clinical presentations of the mother as defined by Kucirka et al.[4]
[Table 1] shows the classification system of maternal disease severity.
Table 1
Maternal SARS-CoV-2 infection severity described by Kucirka et al
Disease severity
|
Mild
|
Moderate
|
Severe
|
Symptoms
|
Asymptomatic
or
fever, cough, myalgia
or
loss of taste or smell
|
Fever >39°C
Dyspnea
Tachypnea
|
Dyspnea
Tachypnea
Septic shock
Hypotension
Multiorgan failure
|
Respiratory difficulty
|
No
|
Yes
|
Yes
|
Computed tomography of chest
|
Normal
|
Lower respiratory tract infection (atypical pneumonia)
|
Lower respiratory tract infection (atypical pneumonia)
|
Oxygen saturation at room air
|
≥93%
|
≥93%
|
<93%
|
Ventilatory support or high flow nasal canula
|
No
|
No
|
Yes
|
Maternal clinical and laboratory data, neonatal demographical data, clinical findings, and neonatal morbidities including respiratory distress syndrome (RDS), neonatal sepsis, necrotizing enterocolitis (NEC), intraventricular hemorrhage (IVH), retinopathy of prematurity (ROP), bronchopulmonary dysplasia (BPD), and mortality were all recorded. Turkish Neonatology Society (TNS) RDS guideline was used for the diagnosis and treatment of RDS.[12] BPD was determined according to 2001 National Institute of Child Health and Human Development consensus statement as requirement for supplemental oxygen for the first 28 days of life and was classified into three groups in terms of BPD severity (mild, moderate, and severe) depending on the duration and level of supplemental oxygen and mechanical ventilatory support at 36 weeks of postmenstrual age.[13] NEC was diagnosed considering clinical and radiographic findings and was classified according to modified Bell's criteria.[14] Volpe classification was used for IVH staging.[15] ROP was classified according to The International Classification of Retinopathy of Prematurity.[16] Neonatal sepsis was diagnosed according to the criteria of TNS neonatal infections diagnosis and treatment guideline.[17]
Statistical analysis was performed by IBM SPSS 22.0 (IBM SPSS for Windows version 22, Armonk, NY). The consistency of continuous variables to normal distribution was tested by using the Shapiro–Wilk test. Descriptive statistics are given as mean ± standard deviation, median (minimum–maximum) for continuous variables and n (%) for categorical variables. Mean values were compared by using one-way ANOVA and Tukey's HSD post hoc analysis tests and categorical values compared by using Fischer's exact tests, p-value of less than 0.05 was considered significant.
Results
A total of 40 pregnant women with a positive RT-PCR test for SARS-CoV-2 were included to the study. In total, 20 (50%) of 40 pregnant women had mild symptoms, whereas 12 (30%) had moderate and 8 (20%) had severe COVID-19 disease. Maternal lymphocyte and platelet count were significantly lower if the mother had severe disease. Maternal fibrinogen, lactate dehydrogenase, Ferritin, C-reactive protein, and D-dimer levels were significantly higher in the presence of moderate or severe maternal disease. One of the mothers with severe disease died within 3 days after admission due to multiorgan failure. Mortality rate for pregnant women infected with SARS-CoV-2 was 2.5%. [Table 2] shows the maternal data of three groups.
Table 2
Laboratory findings and clinical outcome of pregnant women with SARS-CoV-2 infection
|
Mild
(n = 20)
|
Moderate
(n = 12)
|
Severe
(n = 8)
|
White blood cell count (×109 cells/L) Mean ± SD
|
10.17 ± 3.21
|
8.56 ± 2.78[a]
|
10.61 ± 10.59[a]
|
Lymphocyte count (×109 cells/L)
Mean ± SD
|
1.43 ± 0.51
|
1.31 ± 0.55
|
0.86 ± 0.26[a]
|
Platelet count (×109 cells/L)
Mean ± SD
|
271.66 ± 109
|
224.35 ± 89.28[a]
|
186.87 ± 76.82[a]
|
Lactate dehydrogenase (U/L)
Mean ± SD
|
229 ± 47.8
|
272 ± 69.2[a]
|
359 ± 127[a]
|
C-reactive protein (mg/dL)
Mean ± SD
|
2.7 ± 0.3
|
10.3 ± 0.7[a]
|
10 ± 0.7[a]
|
Fibrinogen (mg/dL)
Mean ± SD
|
491 ± 99
|
567 ± 83[a]
|
566 ± 223[a]
|
Ferritin (µg/L)
Mean ± SD
|
58 ± 53
|
99.3 ± 49[a]
|
376 ± 454[a]
|
Prothrombin time (sec)
Mean ± SD
|
7.6 ± 0.2
|
8.0 ± 0.31[a]
|
8.2 ± 0.5[a]
|
Activated partial thromboplastin time (s)
Mean ± SD
|
32.4 ± 4.5
|
32.6 ± 3.7
|
38.4 ± 4.5[a]
|
International normalized ratio
Mean ± SD
|
0.85 ± 0.15
|
0.89 ± 0.03
|
0.91 ± 0.05
|
D-dimer (µg/mL)
Mean ± SD
|
1.32 ± 0.15
|
1.99 ± 1.15[a]
|
2.16 ± 2.09[a]
|
Duration of hospital stay (d)
median (range)
|
2 (2–7)
|
2 (2–10)
|
15.5 (3–49)
|
Maternal mortality
n (%)
|
0
|
0
|
1 (12.5)
|
Abbreviation: SD, standard deviation.
a One-way analysis of variance, p < 0.05.
The mean gestational age and birth weight of infants were significantly lower in infants born to mothers with either moderate or severe disease. The need of postnatal resuscitation was higher, and Apgar's scores were lower in infants born to mothers with severe SARS-CoV-2 infection. The development of respiratory difficulty was significantly higher in infants born to moderate or severe disease. [Table 3] shows the demographical data of neonates.
Table 3
Demographical data, morbidities, and mortalities of newborns with regard to the severity of maternal infection
Maternal disease
severity
|
Mild
(n = 20)
|
Moderate
(n = 12)
|
Severe
(n = 8)
|
Gestational age
(wk)
|
37.8 ± 3.2
(26–41)
|
35.1 ± 3.1[a]
(28–39.4)
|
32.2 ± 5.8[a]
(23–38.2)
|
Birth weight
(g)
|
3,123 ± 758
|
2,578 ± 623[a]
|
2,268 ± 1,203[a]
|
Preterm birth, n (%)
|
4 (20)
|
9 (75)[b]
|
5 (62.5)[b]
|
Postnatal resuscitation need, n (%)
|
1 (5)
|
2 (16.6)
|
6 (75)[b]
|
Apgar's scores
median (range)
|
|
|
|
1 min
|
8 (4–9)
|
8 (3–8)
|
5 (1–8)
|
5 min
|
9 (9–10)
|
9 (7–9)
|
7 (3–9)
|
Respiratory difficulty,
n (%)
|
3 (15)
|
7 (58.3)[b]
|
5 (62.5)[b]
|
Duration of mechanical
|
|
|
|
Ventilation (h)
|
0
|
0
|
321[a]
|
Median (range)
|
(0–480)
|
(0–240)
|
(0–936)
|
Respiratory distress syndrome,
n (%)
|
0
|
0
|
5 (62.5)[b]
|
Endotracheal surfactant, n (%)
|
0
|
0
|
5 (62.5)[b]
|
Neonatal sepsis, n (%)
|
3 (15)
|
8 (66.7)[b]
|
5 (62.5)[b]
|
Patent ductus arteriosus, n (%)
|
1 (5)
|
1 (8.3)
|
3 (37.5)[b]
|
Intraventricular hemorrhage ≥grade 2,
n (%)
|
1 (5)
|
1 (8.3)
|
2 (25)[b]
|
Necrotizing enterocolitis ≥grade 2, n (%)
|
0
|
0
|
1 (12.5)[b]
|
Bronchopulmonary dysplasia (moderate/severe), n (%)
|
0
|
1 (8.3)
|
2 (25)[b]
|
Mortality, n (%)
|
0
|
0
|
1 (12.5)
|
Exclusive breast feeding, n (%)
|
18 (90)[b]
|
10 (83.3)[b]
|
4 (50)
|
Duration of hospital stay
|
|
|
|
Stay (d)
|
2
|
7
|
21.5[a]
|
Median (range)
|
(1–68)
|
(2–57)
|
(2–76)
|
a One-way analysis of variance, p < 0.05.
b Fischer's exact test, p < 0.05.
About 18 (45%) of 40 infants were born as preterm birth. Preterm birth rates were higher in the presence of moderate or severe maternal disease. However, 65% (n = 26) of all infants were asymptomatic after birth, and 35% (n = 14) infants developed respiratory difficulty. Out of 14 infants who developed respiratory difficulty, 3 of them were term infants and 11 were preterm. None of the term infants needed mechanical ventilation, but nine preterm infants were mechanically ventilated. The duration of mechanical ventilation and total hospital stay were significantly higher in infants born to mothers with severe disease. There was no difference in terms of morbidities such as neonatal sepsis, PDA, BPD, IVH, or NEC between groups. One of the infants whose mother had severe disease was died. Exclusive breastfeeding rates at discharge for all infants were 80% (n = 32). Neonatal morbidities are also shown in [Table 3].
Neonates born to mothers with severe disease had significantly lower total leucocyte count, but lymphocyte counts were similar with the other groups. Activated partial thromboplastin time and international normalized ratio were found to be significantly higher in infants born to mothers with severe SARS-CoV-2 infection. Laboratory findings of infants are detailed in [Table 4]. All the individual characteristics of neonates are shown in [Table 5].
Table 4
Laboratory findings of neonates in the study population
|
Mild
(n = 20)
|
Moderate
(n = 12)
|
Severe
(n = 8)
|
White blood cell count (×109 cells/L)
Mean ± SD
|
17.43 ± 5.58
|
16.06 ± 6.94
|
11.27 ± 4.2[a]
|
Lymphocyte count (×109 cells/L)
Mean ± SD
|
4.10 ± 1.44
|
4.27 ± 2.01
|
4.02 ± 1.26
|
Platelet count (×109 cells/L)
Mean ± SD
|
277.2 ± 74.47
|
275.41 ± 67.19
|
246.50 ± 99.94
|
C-reactive protein (mg/dL)
Mean ± SD
|
1.83 ± 3.99
|
0.83 ± 1.34
|
0.43 ± 0.19
|
Fibrinogen (mg/dL)
Mean ± SD
|
233.1 ± 82
|
189.8 ± 160
|
220 ± 104
|
Prothrombin time (s)
Mean ± SD
|
13.53 ± 4.67
|
13.49 ± 2.31
|
13.97 ± 2.52
|
Activated partial thromboplastin time (s)
Mean ± SD
|
38.5 ± 6.61
|
46.4 ± 15.5
|
51.1 ± 11.12[a]
|
International normalized ratio
Mean ± SD
|
1.24 ± 0.16
|
1.4 ± 0.38
|
1.5 ± 0.26[a]
|
D-dimer (µg/mL)
Mean ± SD
|
2.67 ± 1.92
|
1.83 ± 2.46
|
4.2 ± 3.13
|
Abbreviation: SD, standard deviation.
a One-way analysis of variance, p < 0.05.
Table 5
Neonatal characteristics of all infants
Patient no.
|
Gestational age
(wk)
|
Birth weight
(grams)
|
Maternal disease severity
|
Maternal outcome
|
Postnatal resuscitation need
|
Mechanical ventilation invasive/noninvasive
|
Neonatal morbidities
PDA/IVH/NEC/BPD
|
Hospital stay
(d)
|
Neonatal outcome
|
1
|
37
|
3,375
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
2
|
38.3
|
2,790
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
3
|
39
|
3,665
|
Mild
|
Discharge
|
No
|
−/+
|
No/No/No/No
|
3
|
Discharge
|
4
|
37.9
|
2,890
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
5
|
38.2
|
3,315
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
6
|
41
|
3,770
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
7
|
38.9
|
3,375
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
1
|
Discharge
|
8
|
40
|
3,730
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
9
|
41
|
4,570
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
10
|
39.6
|
3,720
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
1
|
Discharge
|
11
|
39.6
|
3,190
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
12
|
39.7
|
2,750
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
1
|
Discharge
|
13
|
37
|
3,500
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
7
|
Discharge
|
14
|
39.3
|
3,520
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
3
|
Discharge
|
15
|
38
|
3,590
|
Mild
|
Discharge
|
No
|
−/+
|
No/No/No/No
|
7
|
Discharge
|
16
|
38.6
|
2,760
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
17
|
36
|
2,750
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
18
|
36.7
|
3,770
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
3
|
Discharge
|
19
|
35.7
|
2,430
|
Mild
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
4
|
Discharge
|
20
|
26
|
800
|
Mild
|
Discharge
|
Yes
|
+/+
|
Yes/Grade 2/No/No
|
68
|
Discharge
|
21
|
38.2
|
3,635
|
Moderate
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
5
|
Discharge
|
22
|
37.6
|
2,645
|
Moderate
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
1
|
Discharge
|
23
|
39.4
|
3,470
|
Moderate
|
Discharge
|
No
|
-/+
|
No/No/No/No
|
7
|
Discharge
|
24
|
34.4
|
2,600
|
Moderate
|
Discharge
|
Yes
|
+/+
|
No/No/No/No
|
14
|
Discharge
|
25
|
35
|
2,420
|
Moderate
|
Discharge
|
No
|
−/+
|
No/No/No/No
|
7
|
Discharge
|
26
|
36.3
|
2,715
|
Moderate
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
7
|
Discharge
|
27
|
36.4
|
2,705
|
Moderate
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
28
|
36.4
|
2,700
|
Moderate
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
29
|
33.4
|
2,765
|
Moderate
|
Discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
30
|
31.7
|
1,975
|
Moderate
|
Discharge
|
No
|
+/+
|
No/No/No/No
|
10
|
Discharge
|
31
|
34.4
|
1,960
|
Moderate
|
Discharge
|
No
|
+/+
|
No/No/No/No
|
19
|
Discharge
|
32
|
28
|
1,350
|
Moderate
|
Discharge
|
Yes
|
+/+
|
Yes/Grade 2/No/Yes
|
57
|
Discharge
|
33
|
38
|
3,310
|
Severe
|
ICU admission, HHHFNC, discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
34
|
38.2
|
3,625
|
Severe
|
ICU admission, HHHFNC, discharge
|
No
|
−/−
|
No/No/No/No
|
2
|
Discharge
|
35
|
36.7
|
3,400
|
Severe
|
ICU admission, N-CPAP, discharge
|
No
|
−/−
|
No/No/No/No
|
3
|
Discharge
|
36
|
36.2
|
3,100
|
Severe
|
ICU admission, HHHFNC, discharge
|
Yes
|
+/+
|
No/No/No/No
|
7
|
Discharge
|
37
|
29
|
1,200
|
Severe
|
ICU admission, Endotracheal intubation, exitus
|
Yes
|
+/+
|
Yes/Grade 1/Grade 1/Yes
|
76
|
Discharge
|
38
|
27
|
1,300
|
Severe
|
ICU admission, endotracheal intubation, discharge
|
Yes
|
+/+
|
Yes/Grade 2/No/Yes
|
62
|
Discharge
|
39
|
29.9
|
1,580
|
Severe
|
ICU admission, endotracheal intubation, discharge
|
Yes
|
+/+
|
No/No/No/No
|
36
|
Discharge
|
40
|
23
|
630
|
Severe
|
ICU admission, Endotracheal intubation, discharge
|
Yes
|
−/+
|
Yes/Grade 4/No/No
|
11
|
Exitus
|
Abbreviations: BPD, bronchopulmonary dysplasia; CPAP, continuous positive airway pressure; HHHFNC, heated humidified high flow nasal cannula; ICU, intensive care unit; IVH, intraventricular hemorrhage; NEC, necrotizing enterocolitis; PDA, patent ductus arteriosus.
Discussion
This retrospective study showed SARS-CoV-2 infection had an adverse impact on neonatal outcomes proportionally with the maternal disease severity including increased prematurity rates, postnatal resuscitation need, prolonged hospital stay, and longer ventilatory support requirement in infants born to mothers with moderate or severe disease.
SARS-CoV-2 is a capsulated, single-stranded RNA virus. Immunological response relies on working immune system.[18] SARS-CoV-2 mainly transmitted by respiratory droplets and close person-to-person contact. Infected aerosols enter body and infects pulmonary cells via the SARS-CoV-2 receptor angiotensin converting enzyme 2 (ACE2) for spike (S) protein.[19] Pregnancy is a relatively immune suppressant status which allows the growth of semiallogeneic fetus and results in an altered immune response to infections.[19] During pregnancy, T-helper (Th) 1 associated cell-mediated immune response is downregulated, and this results with T-helper 2 dominant humoral response and allows for the immune tolerance to developing fetus.[20] These immune changes could have impact on maternal SARS-CoV-2 severity.[4] Th1 mediated cellular immunity plays an important role in lung damage and Th1 associated cytokines such as interleukin-6 has been associated with poor prognosis in SARS-CoV-2 infection. Therefore, it has been sought that Th2 mediated dominant humoral immune response in pregnancy could contribute to mild disease severity.[21]
[22] In contrast, it is also hypothesized that pregnant women are in a pro-inflammatory state in the first and third trimester so they could be more susceptible to infection. A national study from United States suggests that pregnant women infected with SARS CoV-2 are more likely develop severe complications compared with their reproductive age nonpregnant counterparts.[23] Another study comparing pregnant to nonpregnant reproductive age women showed no statistically significant difference in rates of intensive care unit admission.[24] The incidence of SARS-CoV-2 infection severity in pregnant women was reported in different studies as 13.9%,[25] 15%[26] and it was similar to what was described in the literature for the nonpregnant women.[27] In accordance, the incidence of severe SARS-CoV-2 infection was found 20% in the present study.
The possibility of vertical transmission of SARS-CoV-2 from infected mother to the fetus is still a debate.[28] ACE2 is the main host receptor for SARS-CoV-2, and ACE2 is expressed in placenta.[29] Initially most of the studies reported no evidence of vertical transmission.[24]
[30]
[31] Recently, one large study from New York City also reported no vertical transmission.[32] There also exists little studies defining cases about vertical transmission. Zeng et al[33] reported documented immunoglobulin M in neonates born to mothers who had SARS-CoV-2 infection. SARS-CoV-2 RT-PCR test positivity was reported as 5% in a cohort study reported from United Kingdom[34] and 1.9% in National Registry for Surveillance and Epidemiology of Perinatal COVID-19 Infection.[35] Turkish Neonatal Society and the national Neo-COVID study from Turkey reported the rate of RT-PCR positivity as 3.3%.[36]
[37] None of neonates in our study group had positive SARS-CoV-2 RT-PCR test. This may be associated with low patient population and RT-PCR timing in our study.
Maternal disease severity was found to be directly proportional with neonatal outcomes in the present study. The birth weight and gestational age of infants born either moderate or severe maternal disease were significantly lower than those born to mothers with mild disease. Therefore, it may be reasonable to state that rates of prematurity increase with the severity of maternal disease. The total prematurity rate was 45% in our study. This was similar to the 47% prematurity rate found in a review.[38] The national Neo-COVID study reported the prematurity rate as 26.4%, which is lower than that found in our study and this sought to be related with low sample size in our study.[37]
Most of the infants (65%) were asymptomatic in our study population. However, 35% of infants (n = 14) developed respiratory difficulty and 78.5% of them were preterm. In consistent with our findings, many papers reported that most of the infants born to mothers with SARS-CoV-2 are asymptomatic.[10]
[39]
[40]
[41] The most common symptoms that are reported in the literature were pneumonia, respiratory distress, fever, feeding intolerance, vomiting, and diarrhea.[39]
[41]
[42] Another study from Turkey evaluating epidemiological and clinical characteristics of neonates with community acquired COVID-19 reported that main complaints and clinical findings in neonates are fever, hypoxemia, cough, tachypnea, poor feeding, and diarrhea.[43] In our study population, the most common symptom was respiratory difficulty and pneumonia; in contrast with the literature, there were neither fever nor diarrhea were defined in our patients. This may be associated with the patient characteristics.
The infants born to mothers with moderate or severe SARS-CoV-2 infection had increased postnatal resuscitation need, higher respiratory problems, increased ventilatory support requirement, and had longer hospital stay. Only one preterm infant with gestational age of 23 weeks died in the study group whose mother had severe SARS-CoV-2 infection. This may either be associated extreme prematurity or effect of severe maternal disease.
The associations between maternal SARS-CoV-2 infection severity and neonatal characteristics are not well defined. One recent study evaluating the associations reported that women with severe disease had infants with a lower gestational age at delivery; other characteristics of neonates were similar except maternal disease severity was associated with increased risk of neonatal hyperbilirubinemia requiring phototherapy.[32] Another study reported that women with severe disease had more adverse obstetrical outcomes, including lower gestational age, higher cesarean delivery, and their infants had higher rates of neonatal intensive care unit admission, RDS, and IVH in contrast to controls.[44] In accordance, this study associated maternal disease severity with decreased gestational age and birth weight. It also reports increased need of postnatal resuscitation, hospital stay, and ventilatory support requirement in the infants born to mothers with severe SARS-CoV-2 infection.
Low sample size, retrospective design, and single referral center experience constitute the main limitations of this study. Also, no placental tissue, amniotic fluid, nor breast milk were tested for SARS-CoV-2 to demonstrate vertical transmission.
In conclusion, to the best of our knowledge, this is one of the exceptional studies evaluating the severity of maternal SARS-CoV-2 infection and its impact on newborn infants. Even no vertical transmission was shown; maternal disease severity had adverse effects on neonatal outcomes. The severity of maternal disease was found to be associated with increased rates of prematurity, requirement of postnatal resuscitation, prolonged hospital stay, and longer ventilatory support.
Even if the newborn infants were not infected with SARS-CoV-2, adverse outcomes sought to be related with inflammatory status of mother, increased preterm birth rates, and increased preterm morbidities.