Expression of Estrogen Receptors Alpha (ER-α), Beta (ER-β), and G Protein-Coupled Receptor 30 (GPR30) in Testicular Tissue of Men with Klinefelter Syndrome

R. L. Bernardino; M. G. Alves; J. Silva; A. Barros; L. Ferraz; M. Sousa; R. Sá; P. F. Oliveira

doi:10.1055/s-0042-105151

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2016; 48(06): 413-415
DOI: 10.1055/s-0042-105151

Immediate Interest

Expression of Estrogen Receptors Alpha (ER-α), Beta (ER-β), and G Protein-Coupled Receptor 30 (GPR30) in Testicular Tissue of Men with Klinefelter Syndrome

R. L. Bernardino

¹Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal

,

M. G. Alves

²CICS – UBI – Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal

,

J. Silva

³Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal

,

A. Barros

³Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal

⁴Department of Genetics, Faculty of Medicine, University of Porto, Portugal

,

L. Ferraz

⁵Department of Urology, Hospital Center of Vila Nova de Gaia, Vila Nova de Gaia, Portugal

,

M. Sousa

¹Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal

³Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal

,

R. Sá

¹Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal

,

P. F. Oliveira

¹Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal

⁶Institute of Health Research and Innovation (I3S), University of Porto, Porto, Portugal

› Author Affiliations

Abstract

Men with Klinefelter syndrome (KS) present severe hormonal dysregulation, particularly elevated serum estradiol concentration. Estrogens act through specific receptors and regulate testes development and spermatogenesis. Herein, we evaluated GPR30, ERα, and ERβ mRNA expression in testis of KS men and men with 46XY karyotype by reverse transcriptase and quantitative PCR. ERβ transcripts are the most abundant in testicular tissue of 46XY men. Notably, testicular GPR30 transcription in KS men was approximately 12 times higher. Since GPR30 is essential to mediate estrogen effects over steroidogenesis, our data illustrate that GPR30 may underpin the testicular alterations observed in KS men.

Key words

estrogen receptors - Klinefelter syndrome - spermatogenesis - fertility

Full Text

References

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