Download PDF
Drug Res (Stuttg) 2016; 66(08): 420-426
DOI: 10.1055/s-0042-108186
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Reversible Anti-Spermatogenic Effect of Piperine on Epididymis and Seminal Vesicles of Albino Rats

G. Chinta
1   DBT-Interdisciplinary Program in Life Sciences, Pondicherry University, Puducherry, India
,
L. Periyasamy
2   Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, India
› Author Affiliations
Further Information

Publication History

received 29 March 2016

accepted 02 May 2016

Publication Date:
09 June 2016 (online)

Also available at
    Permissions and Reprints

Abstract

Background: We have recently proved the interactions of piperine with androgen receptor and androgen binding protein. The present study was aimed to evaluate the antifertility effect of piperine on male albino rats after the treatment period i. e., after 60 days and withdrawal period i. e., after 120 days.

Materials and Methods: Adult male rats were divided into 4 groups (n=12). Group I: Control: Rats were given vehicle p.o i. e., 0.5% carboxy methyl cellulose (CMC) in normal saline daily for 60 days, Group II: Rats were treated with piperine suspended in 0.5% CMC at a dose of 10 mg/kg daily/60 days. Group III: Rats were treated with piperine suspended in 0.5% CMC at a dose of 10 mg/kg on every 4th day for 60 days. Group IV: Rats were treated with piperine suspended in 0.5% CMC at a dose of 10 mg/kg on every 7th day for 60 days.

Results: Piperine significantly altered the epididymal sperm count, motility, viability, weight of the epididymis, cauda, caput, corpus and seminal vesicles. It also exhibited negative impact on biochemical markers via decreasing epididymal sialic acid levels, seminal fructose content, epididymal anti-oxidant enzyme activities of super oxide dismutase (SOD), catalase (CAT) and by increasing the malondialdehyde content after the treatment period. Histopathological observations also supported the above findings. All the altered values were reinforced after the withdrawal period.

Conclusion: From the results of this study, we can conclude that piperine has the potential to become a good lead for the reversible male oral contraceptive research.

Key words

piperine - reversible male oral contraceptive - anti-fertility activity - Withdrawal period - Anti-oxidant enzymes - Sialic acid levels
 

  • References

  • 1 Rabe T. Contraception - Update and trends. J Reprod Endokrinol 2010; 7: 18-38
    PubMedGoogle Scholar
  • 2 Stalin C, Vivekanandan C, Bhavya E. Medicinal plants with anti-fertility activity: An overview. Global J Med Res 2013; 13: 1-4
    PubMedGoogle Scholar
  • 3 Guptha RS, Sharma R. A review on plant exhibiting anti-fertility activities. Nat Prod Rad 2006; 5: 389-410
    PubMedGoogle Scholar
  • 4 Pasqualotto FF, Lucon AM, Pasqualotto EB et al. Trends in male contraception. Rev Hosp Clin Fac Med Sao Paulo 2003; 58: 275-283
    CrossrefPubMedGoogle Scholar
  • 5 Pavankumar S, Gourav L, Deepak KS et al. Anti-fertility activities of traditional medicinal plants in male with emphasis on their mode action: A review. J Glob Sci 2015; 1: 1165-1179
    PubMedGoogle Scholar
  • 6 Rajandeep K, Anil S, Ravinder K et al. Rising Trends towards Herbal Contraceptives. J Nat Prod & Plant Res 2011; 1: 5-12
    PubMedGoogle Scholar
  • 7 Johri RK, Zutshi U. An Ayurvedic formulation ‘Trikatu’ and its constituents. J Ethnopharmacol. 1992; 37: 85-91
    CrossrefPubMedGoogle Scholar
  • 8 Chinta G, Charles MRC, Klopčič I et al. In Silico and In Vitro Investigation of the Piperineʼs Male Contraceptive Effect: Docking and Molecular Dynamics Simulation Studies in Androgen-Binding Protein and Androgen Receptor. Planta Med 2015; 81: 804-812
    Article in Thieme ConnectPubMedGoogle Scholar
  • 9 Kulkarni SK, Bhutani MK, Bishnoi M. Antidepressant activity of curcumin: involvement of serotonin and dopamine system. Psychopharmacol (Berl) 2008; 201: 435-442
    CrossrefPubMedGoogle Scholar
  • 10 Li S, Wang C, Wei Wang M et al. Anti-Depressant like effects of piperine in chronic mild-stress treated mice and its possible mechanisms. Life Sci 2007; 80: 1373-1381
    CrossrefPubMedGoogle Scholar
  • 11 Li S, Wang C, Li W et al. Antidepressant-like effects of piperine and its derivative, antiepilepsirine. J Asian Nat Prod Res 2010; 9: 421-430
    PubMedGoogle Scholar
  • 12 Panda S, Kar A. Piperine lowers the serum concentrations of thyroid hormones, glucose and hepatic 5’D activity in adult male mice. Horm Metab Res 2003; 35: 523-526
    Article in Thieme ConnectPubMedGoogle Scholar
  • 13 Park BS, Son DJ, Park YH et al. Antiplatelet effects of acidamides isolated from the fruits of Piper longum L. Phytomed 2007; 14: 853-855
    CrossrefPubMedGoogle Scholar
  • 14 Atal S, Agrawal RP, Vyas S et al. Evaluation of the effect of piperine per se on blood glucose level in alloxan-induced diabetic mice. Acta Pol Pharm 2012; 69: 965-969
    PubMedGoogle Scholar
  • 15 Atal S, Atal S, Vyas S et al. Bio-enhancing effect of Piperine with Metformin on lowering blood glucose level in Alloxan induced diabetic mice. Pharmacog Res 2016; 8: 56-60
    PubMedGoogle Scholar
  • 16 Mujumdar AM, Dhuley JN, Deshmukh VK et al. Anti-inflammatory activity of piperine. Jpn J Med Sci Biol 1990; 43: 95-100
    PubMedGoogle Scholar
  • 17 Veerareddy PR, Vobalaboina V, Nahid A. Formulation and evaluation of oil-in-water emulsions of piperine in visceral leishmaniasis. Pharmazie 2004; 59: 194-197
    PubMedGoogle Scholar
  • 18 Samykutty A, Shetty AV, Dakshinamoorthy G et al. Piperine, a Bioactive Component of Pepper Spice Exerts Therapeutic Effects on Androgen Dependent and Androgen Independent Prostate Cancer Cells. PLoS One 2013; 8: e65889
    CrossrefPubMedGoogle Scholar
  • 19 Kim SH, Lee YC. Piperine inhibits eosinophil infiltration and airway hyperresponsiveness by suppressing T cell activity and Th2 cytokine production in the ovalbumin-induced asthma model. J Pharm Pharmacol 2009; 61: 353-359
    CrossrefPubMedGoogle Scholar
  • 20 El Hamss R, Idaomar M, Alonso-Moraga A et al. Antimutagenic properties of bell and black peppers. Food Chem Toxicol 2003; 41: 41-47
    CrossrefPubMedGoogle Scholar
  • 21 Bajad S, Bedi KL, Singla AK et al. Antidiarrhoeal activity of piperine in mice. Planta Med 2001; 67: 284-287
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Koul IB, Kapil A. Evaluation of the liver protective potential of piperine, an active principle of black and long peppers. Planta Med 1993; 59: 413-417
    Article in Thieme ConnectPubMedGoogle Scholar
  • 23 Zhao JQ, Du GZ, Xiong YC et al. Attenuation of beryllium induced hepatorenal dysfunction and oxidative stress in rodents by combined effect of gallic acid and piperine. Arch Pharm Res 2007; 30: 1575-1583
    CrossrefPubMedGoogle Scholar
  • 24 Badmadev V, Majid M, Norkus EP. Piperine, An alkaloid derived from Black pepper increases serum response of Beta carotene during14 day Oral Beta carotene supplementation. Nutri Res 1999; 19: 381-388
    CrossrefPubMedGoogle Scholar
  • 25 Nirala SK, Bhadauria M, Mathur R et al. Influence of alpha-tocopherol, propolis and piperine on therapeutic potential of tiferron against beryllium induced toxic manifestations. J Appl Toxicol 2008; 28: 44-54
    CrossrefPubMedGoogle Scholar
  • 26 Lambert JD, Hong J, Kim DH et al. Piperine enhances the bioavailability of the tea polyphenol (-)-epigallocatechin-3-gallate in mice. J Nutr 2004; 134: 1948-1952
    PubMedGoogle Scholar
  • 27 Han HK. The effects of black pepper on the intestinal absorption and hepatic metabolism of drugs. Expert Opin Drug Metab Toxicol 2011; 7: 721-729
    CrossrefPubMedGoogle Scholar
  • 28 Patil UK, Singh A, Chakraborthy AK. Role of piperine as a Bioavailability Enhancer. Int J Recent Adv In Pharmaceut Res 2011; 4: 16-23
    PubMedGoogle Scholar
  • 29 Chinta G, Syed SB, Coumar SM et al. Piperine: A Comprehensive review of its preclinical and clinical investigations. Current Bioact Comp 2015; 11: 156-169
    PubMedGoogle Scholar
  • 30 Malini T, Manimaran RR, Arunakaran J et al. Effects of piperine on testis of albino rats. J Ethnopharmacol 1999; 64: 219-225
    CrossrefPubMedGoogle Scholar
  • 31 D’cruz SC, Mathur PP. Effect of piperine on the epididymis of adult male rats. Asian J Androl 2005; 7: 363-368
    CrossrefPubMedGoogle Scholar
  • 32 D’Cruz SC, Vaithinathan S, Saradha B et al. Piperine activates testicular apoptosis in adult rats. J Biochem Mol Toxicol 2008; 22: 382-388
    CrossrefPubMedGoogle Scholar
  • 33 Piyachaturawat P, Glinsukon T, Toskulkao C. Acute and subacute toxicity of piperine in mice, rats and hamsters. Toxicol Lett 1983; 16: 351-359
    CrossrefPubMedGoogle Scholar
  • 34 Gangadharan B, Murugan MA, Mathur PP. Effect of methoxychlor on antioxidant system of goat epididymal sperm in vitro. Asian J Androl 2001; 3: 285-288
    PubMedGoogle Scholar
  • 35 Cooper TG. WHO Laboratory manual for the examination and processing of human semen. 5 Ed Switzerland: WHO Press; 2010
    Google Scholar
  • 36 Lombart C, Winzler RJ. Isolation and characterization of canine submaxillary mucin. Biochem J 1972; 128: 975-977
    CrossrefPubMedGoogle Scholar
  • 37 Nixon DA. The determination of fructose in biological fluids using anthrone. Clin Chim Acta 1969; 26: 167-169
    CrossrefPubMedGoogle Scholar
  • 38 Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974; 47: 469-474
    CrossrefPubMedGoogle Scholar
  • 39 Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972; 47: 389-394
    CrossrefPubMedGoogle Scholar
  • 40 Devasagayam TP, Tarachand U. Decreased lipid peroxidation in the rat kidney during gestation. Biochem Biophys Res Commun 1987; 145: 134-138
    CrossrefPubMedGoogle Scholar
  • 41 Arroteia KF, Garcia PV, Barbieri MF et al. The Epididymis: Embryology, structure, function and its role in fertilization ans infertility. In: Pereira LAV. (ed) Embryolog-updates and highlights on classic topics. University campus Stephri: Intech. 2012: 41-66
    CrossrefGoogle Scholar
  • 42 Marib EN. The Reproductive System. In: Beauparlant S. (Ed) Essentials of Human Anatomy and Physiology. United States: Pearson; 2015: 37
    Google Scholar
  • 43 Roberts M, Jarvi K. Steps in the investigation and management of low semen volume in the infertile man. Can Urol Assoc J 2009; 3: 479-485
    PubMedGoogle Scholar
  • 44 Selen S, Tortora GJ, Derrickson BH. The reproductive system. In: Gerrerd J, Tortora Derricson BH. (Ed) Principles of Anatomy and Physiology. USA: John Willey and Sons; 2012: 1029-1064
    Google Scholar
  • 45 Vetter CM, Miller JE, Crawford LM et al. Comparison of motility and membrane integrity to assess rat sperm viability. Reprod Toxicol 1998; 12: 105-114
    CrossrefPubMedGoogle Scholar
  • 46 Anbalagan J, Kanagaraj P, Srinivasan N et al. Effect of polychlorinated bisphenyl, Arachlor 1254 on rat epididymis. Indian J Medical Res 2003; 118: 236-242
    PubMedGoogle Scholar
  • 47 Joshi SC, Sharma A, Sharma P et al. Contraceptive Efficacy of Citrullus colocynthis Methanolic Extract in Male Rats. American J Phytomed & Clinic Therapeut 2014; 2: 229-241
    PubMedGoogle Scholar
  • 48 Gupta RS, Kachhawa JB, Chaudhary R. Antifertility effects of methanolic pod extract of Albizzia lebbeck (L.) Benth in male rats. Asian J Androl 2004; 6: 155-159
    PubMedGoogle Scholar
  • 49 Olayinka E, Ore A. Hepatotoxicity, nephrotoxicity and oxidative Stress in rat testis following exposure to Haloxyfop-p-methyl ester, an Aryloxyphenoxypropionate herbicide. Toxics 2015; 3: 373-389
    CrossrefPubMedGoogle Scholar
  • 50 Ghiasi Ghalehkandi J, Hassanpour S, Issabeagloo E et al. Assessment of the effects of red onion (Allium cepa Linn) juice on semen oxidative status compared to Zn sulfate in rats. Animal reproduction 2015; 12: 298-304
    PubMedGoogle Scholar
  • 51 Abarikwu SO, Otuechere CA, Ekor M et al. Ameliorates Cyclophosphamide-induced Reproductive Toxicity in Male Rats. Toxicol Int 2012; 19: 207-214
    CrossrefPubMedGoogle Scholar
  • 52 Alagammal M, Sakthidevi G, Mohan VR. Anti-fertility activity of whole plant extracts of Polygola rosmanifolia wight & Arn against male albino rats. Journal of advanced pharmaceutical sciences 2013; 3: 386-393
    PubMedGoogle Scholar