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Planta Med 2017; 83(01/02): 151-157
DOI: 10.1055/s-0042-109779
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Intermedin A, a New Labdane Diterpene Isolated from Alpinia intermedia, Prolonged the Survival Time of P-388D1 Tumor-Bearing CDF1 Mice

Lih-Geeng Chen
1   Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi, Taiwan
,
Pei-Jung Su
2   Graduate Institute of Pharmacognosy Science, School of Pharmacy, Pharmacy of College, Taipei Medical University, Taipei, Taiwan
,
Po-Wei Tsai
3   School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
,
Ling-Ling Yang
3   School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
,
Ching-Chiung Wang
2   Graduate Institute of Pharmacognosy Science, School of Pharmacy, Pharmacy of College, Taipei Medical University, Taipei, Taiwan
3   School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
4   Orthopedics Research Center, Taipei Medical University Hospital, Taipei, Taiwan
› Author Affiliations
Further Information

Publication History

received 01 November 2015
revised 15 May 2016

accepted 21 May 2016

Publication Date:
28 June 2016 (online)

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Abstract

Eight ethanolic extracts of indigenous Taiwanese plants of the genus Alpinia were tested for tumor cytotoxicity against AGS, Hep G2, HeLa, KB, and HL-60 cells. Among the 50 % and 95 % EtOH extracts of eight Alpinia species, the cytotoxic effects of Alpinia intermedia leaves were the strongest. When the leaf extract of A. intermedia was partitioned using n-hexane and aqueous solvents, the n-hexane layer showed a greater cytotoxic effect and could prolong the survival time of P-388D1 tumor-bearing CDF1 mice. Two new labdane diterpene derivatives, intermedin A (1) and intermedin B (2), and coronarin E (3) were isolated from the n-hexane layer of A. intermedia. Intermedin A induced apoptosis in HL-60 cells at 30 µg/mL and significantly prolonged the survival time of P-388D1 tumor-bearing CDF1 mice by 48.7 % at 20 mg/kg of body weight. We suggest that intermedin A is a major compound of A. intermedia and has a cytotoxic effect on HL-60 and P-388D1 cells.

Key words

Alpinia intermedia - Zingiberaceae - intermedin A - apoptosis - P-388D1 tumor-bearing CDF1 mice

Supporting Information

 

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