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Planta Med 2016; 82(15): 1374-1380
DOI: 10.1055/s-0042-111520
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

New Sulphated Flavonoids from Tamarix africana and Biological Activities of Its Polar Extract

Manel Karker
1   Laboratory of Aromatic and Medicinal Plants, Biotechnology Center at the Technopole of Borj-Cédria (CBBC), Hammam-Lif, Tunisia
,
Nunziatina De Tommasi
2   Dipartimento di Farmacia, Università di Salerno, Fisciano (SA), Italy
,
Abderrazak Smaoui
3   Laboratory of Extremophile Plants, Biotechnology Center at the Technopole of Borj-Cedria (CBBC), Hammam-Lif, Tunisia
,
Chedly Abdelly
3   Laboratory of Extremophile Plants, Biotechnology Center at the Technopole of Borj-Cedria (CBBC), Hammam-Lif, Tunisia
,
Riadh Ksouri
1   Laboratory of Aromatic and Medicinal Plants, Biotechnology Center at the Technopole of Borj-Cédria (CBBC), Hammam-Lif, Tunisia
,
Alessandra Braca
4   Dipartimento di Farmacia, Università di Pisa, Pisa, Italy
5   Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per la Salute”, Università di Pisa, Pisa, Italy
› Author Affiliations
Further Information

Publication History

received 04 April 2016
revised 23 June 2016

accepted 26 June 2016

Publication Date:
12 July 2016 (online)

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Abstract

The phytochemical investigation of Tamarix africana Poir. (Tamaricaceae) shoot polar extract afforded three new sulphated flavonoids, (2S,4R)-5,7,4′-trihydroxyflavan-4-ol 5,7-disulphate (1), (2S)-5,7,4′-trihydroxyflavan 7-O-sulphate (2), and (2S)-naringenin 4′-O-sulphate (3), together with ten known compounds. Their structures were determined by spectroscopic methods including 1D and 2D NMR analysis and HRMS. Biological activities of the polar extract of T. africana shoots related to its phenolic content were also investigated. A high total phenolic content (151.1 mg GAE/g) was found in the methanol shoot extract, which exhibits strong antioxidant activities using the oxygen radical absorbance capacity method and a skin cell-based assay. Moreover, the shoot extract showed significant anti-inflammatory activity, reducing nitric oxide release by 53.5 % at 160 µg/mL in lipopolysaccharide-stimulated RAW 264.7 macrophages. Finally, T. africana shoot extract inhibited the growth of A-549 lung carcinoma cells, with an IC50 value of 34 µg/mL.

Key words

Tamarix africana - Tamaricaceae - sulphated flavonoids - antioxidant activity - cytotoxicity - anti-inflammatory activity

Supporting Information

 

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