Antimicrobial Activity of Homeopathic Dilutions of Gun Powder 6C and 30C against Staphylococcus aureus

Abstract

Background

Some scientists and celebrities have labeled homeopathy as pseudoscience due to a dearth of fundamental data supporting the efficacy of homeopathic medicines. The majority of homeopathic physicians feel that clinical trials will suffice for homeopathy research. However, the pharmacokinetics and pharmacodynamics of homeopathic medicines cannot be elicited by clinical trials; these questions may be addressed by basic research investigations on homeopathic medications.

Objective

The aim of this study was to explore the antimicrobial activity of homeopathic preparation of Gun powder 6C & 30C through microdilution and disk diffusion assays.

Materials and Methods

The antimicrobial activity of Gun powder 6C & 30C is evaluated through a microdilution assay followed by the Kirby–Bauer disk diffusion (KBDD) method according to the Clinical and Laboratory Standards Institute (CLSIM44-A) with slight modifications. The diameter of the zone of inhibition was measured along with 91% ethanol as negative controls and gentamycin as positive control. This study was repeated three times to check the accuracy and reliability.

Result

The mean and standard deviation of optical density of Gun powder 6C & 30C in 10 µL show a lesser value than ethanol. Gun powder 6C & 30C exhibited significant results against S. aureus after appropriate statistical analysis. The zone of inhibition of Gun powder 6C against Staphylococcus is 25.33 ± 4.16 mm and that of Gun powder 30C is 23.33 ± 3.06. The positive control (gentamycin 10 μg) showed a mean zone of inhibition of 36.33 ± 3.06 and the negative control (91% ethanol) showed a mean zone of inhibition of 6 ± 0.00.

Conclusion

Microdilution and disk diffusion assays indicate that Gun powder 6C & 30C are most potent against S. aureus disorders caused by antimicrobial resistance (AMR). The study suggested that Homoeopathic preparation of Gun powder 6C & 30C is most potent against Staphylococcus aureus disorders caused by antimicrobial resistance (AMR). Hence, Gun powder 6C & 30C may be used as novel, potential drug for antimicrobial resistance disorders which may be less cost effective than current antibiotic treatment.

Keywords

homeopathy - Gun powder - Clinical and Laboratory Standards Institute guidelines - Kirby–Bauer disk diffusion method

Authors' Contributions

K.V. developed the concept and design of the study, prepared the protocol, conducted the research, provided the research materials, collected, and organized the data, analyzed the data, wrote the original manuscript, and supervised the study. S.P was involved in the research works and critical review of the draft manuscript. P.C was involved the research works. C.N was involved in critical review of the draft manuscript.

Publikationsverlauf

Artikel online veröffentlicht:
21. April 2025

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