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Am J Perinatol 2000; Volume 17(Number 05): 243-248
DOI: 10.1055/s-2000-10005
Copyright © 2000 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

FREQUENT PRESENCE OF HELICOBACTER PYLORI GENOME IN THE SALIVA OF PATIENTS WITH HYPEREMESIS GRAVIDARUM

Satoshi Hayakawa1 , Noriko Nakajima2 , Miki Karasaki-Suzuki1 , Haruki Yoshinaga1 , Yasuyuki Arakawa2 , Kazuo Satoh1 , Tatsuo Yamamoto1
  • 1Department of Obstetrics & Gynaecology, Nihon University School of Medicine, Tokyo, Japan
  • 2Third Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan
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Publication History

Publication Date:
31 December 2000 (online)

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ABSTRACT

Recently, possible involvement of Helicobacter pylori (H. pylori) in hyperemesis gravidarum have been reported based on serological studies and the therapeutic effects of antibiotics. In this study, we examined for the presence of H. pylori genome [by (PCR) of saliva] in combination with serological techniques. Thirty-four patients with hyperemesis and 29 normal pregnant subjects were examined for serum anti-H. pylori IgG antibodies and PCR of saliva. By serum antibody test, 16 of 34 hyperemesis patients (47.5%) were positive for anti-H. pylori IgG antibody, while 6 of 29 control subjects (20.6%) were positive (χ2 p < 0.0005). PCR revealed positive H. pylori genome in 21 cases out of 34 hyperemesis (61.8%, 14 of 16 patients positive for H. pylori antibody and 7 of H. pylori-antibody-negative 18 patients) and 8 of 29 control subjects (27.6%) (χ2 p < 0.000001). We suggest chronic infection of H. pylori as one of the important factors on the pathogenesis of hyperemesis gravidarum even though it may not be the single cause of the disorder.

KEYWORD

Helicobacter pylori; Hyperemesis gravidarum - latent gastritis - PCR

REFERENCES

  • 1 Hod M, Orvieto R, Kaplan B, Friedman S, Ovadia J. Hyperemesis gravidarum: a review.  J Reprod Med . 1994;  39 605-612
    PubMedGoogle Scholar
  • 2 Abell T L, Riely C A. Hyperemesis gravidarum.  Gastroenterol North Am . 1992;  21 835-849
    PubMedGoogle Scholar
  • 3 Iatrakis G M, Sakellaropoulos G G, Kourkobas A H, Kabounia S E. Vomiting and nausea in the first 12 weeks of pregnancy.  Psychoter Psychosom . 1988;  49 22-24
    PubMedGoogle Scholar
  • 4 Nakajima N, Hayakawa S, Karasaki-Suzuki M, Chishima F, Arakawa Y, Satoh K. Helicobacter pylori infection: a possible pathogenic factor of hyperemesis gravidarum?.  Proc Japan Assoc Immunol Reprod . 1998;  12 87-88
    PubMedGoogle Scholar
  • 5 Frigo H, Lang C, Reisenberger K, Kolbl H, Hirshl A M. Hyperemesis gravidarum associated with helicobacter pylori seropositivity.  Obstet Gynecol . 1998;  91 615-617
    CrossrefPubMedGoogle Scholar
  • 6 El Younis M C, Abulafia O, Sherer D M. Rapid marked response of severe hyperemesis gravidarum to oral erythromycin.  Am J Perinatol . 1998;  15 533-534
    PubMedGoogle Scholar
  • 7 Jacoby E B, Porter K B. Helicobcater pylori infection and persistent hyperemesis gravidarum.  Am J Perinatol . 1999;  16 85-88
    PubMedGoogle Scholar
  • 8 Peek R M, Miller G G, Tham K T. Detection of Helicobacter pyroli gene expression in human gastric mucosa.  J Clin Microbiol . 1995;  33 28-32
    PubMedGoogle Scholar
  • 9 Dooley C P. Background and historical considerations of Helicobacter pylori.  Gastroenterol Clin North Am . 1993;  22 1-4
    PubMedGoogle Scholar
  • 10 Tytgat G NJ, Rauws E A. Campylobacter pylori and its role in peptic ulcer disease.  Gastroenterol Clin North Am . 1990;  9 183-196
    PubMedGoogle Scholar
  • 11 Forman D. Helicobacter pylori and gastric cancer.  Scand J Gastroenterol . 1996;  31(suppl) 214
    PubMedGoogle Scholar
  • 12 Correa P. Human gastric carcinogenesis: a multistep and multi factorial process-First American cancer society award lecture on cancer epidemiology and prevention.  Cancer Res . 1992;  52 6735-6740
    PubMedGoogle Scholar
  • 13 Creasy R K, Resnik R. eds. Maternal and Fetal Medicine.  Philadelphia: WB Saunders Co; 1984
    Google Scholar
  • 14 Gionchetti P, Varia D, Campieri M. Enhanced mucosal interleukin-6, and -8 in helicobacter pylori positive dyspeptic patients.  Am J Gastroenterol . 1994;  89 883-887
    PubMedGoogle Scholar
  • 15 Noach L A, Bosma N B, Jansen J. Mucosal tumor necrosis factor α, interleukin-1β, and interleukin-8 production in patients with Helicobacter pylori infection.  Scand J Gastroenterol . 1994;  29 425-429
    PubMedGoogle Scholar
  • 16 Ando T, Kusugami K, Ohsuga M. Interleukin-8 activity correlates with histological severity in Helicobacter pylori-associated antral gastritis.  Am J Gastroenterol . 1996;  91 1150-1156
    PubMedGoogle Scholar
  • 17 Mosmann T R, Cherwinski H, Bond M W, Giedlin M A, Coffman R L. Two types of murine helper T cell clone: I. Definition according to profiles of lymphokine activities and secreted proteins.  J Immunol . 1986;  136 2348-2357
    PubMedGoogle Scholar
  • 18 Wegman T G, Hui L in, Guilbert L, Mosmann T R. Bidirectional cytokine interactions in the maternal fetal relationship: is successful pregnancy a Th2 phenomenon?.  Immunol Today . 1993;  14 353-356
    CrossrefPubMedGoogle Scholar
  • 19 Lin H, Mosmann T R, Guilbert L, Tuntipopipat S, Wegmann T G. Synthesis of T helper 2 type cytokines at the maternal-fetal interface.  J Immunol . 1993;  151 4562-4573
    PubMedGoogle Scholar
  • 20 Hill J A, Polger K, Anderson D J. T helper 1 type immunity to trophoblasts in women with recurrent spontaneous abortion.  JAMA . 1995;  2/3 1933-1936
    PubMedGoogle Scholar
  • 21 Hayakawa S, Krasaki-Suzuki M, Itoh T. Effects of paternal lymphocyte immunization on peripheral Th1/Th2 balance and TCR Vβ and Vα repertoire usage of patients with recurrent spontaneous abortions.  Am J Reprod Immunol . 2000;  43 107-115
    CrossrefPubMedGoogle Scholar
  • 22 Fairweather D VI. Nausea and vomiting during pregnancy.  Am J Obstet Gynecol . 1968;  102 135-175
    PubMedGoogle Scholar
  • 23 Megraud F. Epidemiology of helicobacter pyroli infection.  Gastroenterol Clin North Am . 1993;  22 73-87
    PubMedGoogle Scholar
  • 24 Li C, Ha T, Ferguson Jr A D. A newly developed PCR assay H pylori in gastric biopsy, saliva and Feces.  Dig Dis Sci . 1996;  41 2142-2149
    PubMedGoogle Scholar