Assessment of Sperm Competence

ABSTRACT

The predictive values of four categories of established sperm function assays-computer-assisted semen analysis (CASA), induced-acrosome reaction testing, sperm penetration assay, and sperm-zona pellucida binding assays-are still unsure. In this article we examine the evaluation of sperm competence. We propose that assessment of sperm competence should include investigations at the nuclear, organelle, and cytoskeletal levels. In light of this, we discuss the assessment of sperm nuclear integrity as an alternative new method of analysis. We also question the merit of having such tests, whereby it may be an easier choice to direct these patients straight to an assisted reproduction treatment.

KEYWORD

competence - sperm nuclear DNA - sperm chromatin

REFERENCES

• 1 Hull M G. Infertility treatment: relative effectiveness of conventional and assisted conception methods.  Hum Reprod . 1992;  7 785-796
• 2 World Health Organization. WHO Laboratory Manual for Examination of Human Semen Cambridge: Cambridge University Press; 1992
• 3 World Health Organization. WHO Laboratory Manual for Examination of Human Semen Cambridge: Cambridge University Press; 1999
• 4 Tomlinson M J, Kessopoulou E, Barratt C L. The diagnostic and prognostic value of traditional semen parameters.  J Androl . 1999;  20 588-593
• 5 Barratt C L, Tomlinson M J, Cooke I D. Prognostic significance of computerized motility analysis for in vivo fertility.  Fertil Steril . 1993;  60 520-525
• 6 Irvine D S, Macleod I C, Templeton A A, Masterton A, Taylor A. A prospective clinical study of the relationship between the computer-assisted assessment of human semen quality and the achievement of pregnancy in vivo.  Hum Reprod . 1994;  9 2324-2334
• 7 Hargreave T B, Elton R A. Is conventional sperm analysis of any use?.  Br J Urol . 1983;  55 774-779
• 8 Dunphy B C, Neal L M, Cooke I D. The clinical value of conventional semen analysis.  Fertil Steril . 1989;  51 324-329
• 9 Tomlinson M J, Amissah-Arthur J B, Thompson K A, Kasraie J L, Bentick B. Prognostic indicators for intrauterine insemination (IUI): statistical model for IUI success.  Hum Reprod . 1996;  11 1892-1896
• 10 Liu D Y, Baker H W. The proportion of human sperm with poor morphology but normal intact acrosomes detected with Pisum sativum agglutinin correlates with fertilization in vitro.  Fertil Steril . 1988;  50 288-293
• 11 Duncan W W, Glew M J, Wang X J, Flaherty S P, Matthews C D. Prediction of in vitro fertilization rates from semen variables.  Fertil Steril . 1993;  59 1233-1238
• 12 Kruger T F, Menkveld R, Stander F S. Sperm morphologic features as a prognostic factor in in vitro fertilization.  Fertil Steril . 1986;  46 1118-1123
• 13 Hull M G, Savage P E, Bromham D R. Prognostic value of the postcoital test: prospective study based on time-specific conception rates.  Br J Obstet Gynaecol . 1982;  89 299-305
• 14 Polansky F F, Lamb E J. Analysis of three laboratory tests used in the evaluation of male fertility: Bayes' rule applied to the postcoital test, the in vitro mucus migration test, and the zona-free hamster egg test.  Fertil Steril . 1989;  51 215-228
• 15 Oehninger S, Coddington C C, Scott R. Hemizona assay: assessment of sperm dysfunction and prediction of in vitro fertilization outcome.  Fertil Steril . 1989;  51 665-670
• 16 Liu D Y, Baker H W. Tests of human sperm function and fertilization in vitro.  Fertil Steril . 1992;  58 465-483
• 17 Aitken R J, Irvine D S, Wu F C. Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility.  Am J Obstet Gynecol . 1991;  164 542-551
• 18 Zaneveld L J, Jeyendran R S. Sperm function tests.  Infertil Reprod Med Clin North Am Male Infertil . 1992;  3 353-371
• 19 Oehninger S, Franken D R, Sayed E, Barroso G, Kolm P. Sperm function assays and their predictive value for fertilization outcome in IVF therapy: a meta-analysis.  Hum Reprod Update . 2000;  6 160-168
• 20 Hinsch E, Ponce A A, Hagele W. A new combined in-vitro test model for the identification of substances affecting essential sperm functions.  Hum Reprod . 1997;  12 1673-1681
• 21 Clark G F, Patankar M S, Hinsch K D, Oehninger S. New concepts in human sperm-zona pellucida interaction.  Hum Reprod . 1995;  10(Suppl 1) 31-37
• 22 Urner F, Sakkas D. Glucose is not essential for the occurrence of sperm binding and zona pellucida-induced acrosome reaction in the mouse.  Int J Androl . 1996;  19 91-96
• 23 Baker G, Liu D Y, Bourne H. Assessment of the male and preparation of sperm for ARTs. In: Trounson AO, Gardner DK, eds. Handbook of In Vitro Fertilization. 2nd ed. Boca Raton, FL: CRC Press 2000: 99-126
• 24 De Jonge C J. The role of sperm function testing in infertility management. In: Gagnon C, ed. The Male Gamete: From Basic Science to Clinical Applications Vienna, IL: Cache River Press 1999: 371-378
• 25 Kruger T F, Acosta A A, Simmons K F. New method of evaluating sperm morphology with predictive value for human in vitro fertilization.  Urology . 1987;  30 248-251
• 26 Bianchi P G, Manicardi G C, Urner F, Campana A, Sakkas D. Chromatin packaging and morphology in ejaculated human spermatozoa: evidence of hidden anomalies in normal spermatozoa.  Mol Hum Reprod . 1996;  2 139-144
• 27 Sakkas D, Manicardi G C, Bizzaro D, Bianchi P G, Bianchi U. Chromatin packaging anomalies and DNA damage in human sperm: their possible implications in the treatment of male factor infertility. In: Barratt CL, De Jonge CJ, Mortimer D, Parinaud J, eds. Genetics of Human Male Infertility Paris: Editions EDK 1997: 205-221
• 28 Bizzaro D, Manicardi G C, Bianchi P G, Bianchi U, Mariethoz E, Sakkas D. In-situ competition between protamine and fluorochromes for sperm DNA.  Mol Hum Reprod . 1998;  4 127-132
• 29 Balhorn R, Cosman M, Thornton K. Protamine mediated condensation of DNA in mammalian sperm. In: Gagnon C, ed. The Male Gamete: From Basic Science to Clinical Applications Vienna, IL: Cache River Press 1999: 56-70
• 30 Sakkas D, Urner F, Bianchi P G. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection.  Hum Reprod . 1996;  11 837-843
• 31 Lopes S, Jurisicova A, Casper R F. Gamete-specific DNA fragmentation in unfertilized human oocytes after intracytoplasmic sperm injection.  Hum Reprod . 1998;  13 703-708
• 32 Shoukir Y, Chardonnens D, Campana A, Sakkas D. Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence?.  Hum Reprod . 1998;  13 1632-1637
• 33 Sun J G, Jurisicova A, Casper R F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro.  Biol Reprod . 1997;  56 602-607
• 34 Esterhuizen A D, Franken D R, Lourens J G, Prinsloo E, van Rooyen H L. Sperm chromatin packaging as an indicator of in-vitro fertilization rates.  Hum Reprod . 2000;  15 657-661
• 35 Evenson D P, Darzynkiewicz Z, Melamed M R. Relation of mammalian sperm chromatin heterogeneity to fertility.  Science . 1980;  210 1131-1133
• 36 Irvine D S, Twigg J P, Gordon E L, Fulton N, Milne P A, Aitken R J. DNA integrity in human spermatozoa: relationships with semen quality.  J Androl . 2000;  21 33-44
• 37 Manicardi G C, Bianchi P G, Pantano S. Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A₃ accessibility.  Biol Reprod . 1995;  52 864-867
• 38 Sakkas D, Manicardi G C, Tomlinson M. The use of density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies.  Hum Reprod . 2000;  15 1112-1116
• 39 Evenson D P, Jost L K, Marshall D. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic.  Hum Reprod . 1999;  14 1039-1049
• 40 Spano M, Bonde J P, Hjollund H I, Kolstad H A, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility: the Danish First Pregnancy Planner Study Team.  Fertil Steril . 2000;  73 43-50
• 41 Evenson D P, Jost L K, Baer R K, Turner T W, Schrader S M. Individuality of DNA denaturation patterns in human sperm as measured by the sperm chromatin structure assay.  Reprod Toxicol . 1991;  5 115-125
• 42 Schrader S M, Turner T W, Breitenstein M J, Simon S D. Longitudinal study of semen quality of unexposed workers: I. Study overview.  Reprod Toxicol . 1988;  2 183-190
• 43 Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi P G, Bianchi U. Origin of DNA damage in ejaculated human spermatozoa.  Rev Reprod . 1999;  4 31-37
• 44 Sakkas D, Mariethoz E, St John C J. Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway.  Exp Cell Res . 1999;  251 350-355
• 45 Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa.  Hum Reprod . 2000;  15 1338-1344
• 46 Aitken R J, Gordon E, Harkiss D. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa.  Biol Reprod . 1998;  59 1037-1046
• 47 Cummins J M, Jequier A M, Kan R. Molecular biology of human male infertility: links with aging, mitochondrial genetics, and oxidative stress?.  Mol Reprod Dev . 1994;  37 345-362
• 48 Shen H, Ong C. Detection of oxidative DNA damage in human sperm and its association with sperm function and male infertility.  Free Radic Biol Med . 2000;  28 529-536
• 49 Twigg J, Irvine D S, Houston P, Fulton N, Michael L, Aitken R J. Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: protective significance of seminal plasma.  Mol Hum Reprod . 1998;  4 439-445
• 50 Fishel S, Aslam I, Lisi F. Should ICSI be the treatment of choice for all cases of in-vitro conception?.  Hum Reprod . 2000;  15 1278-1283