Antiplatelet Aggregation Principles from Ephemerantha lonchophylla

Abstract

Bioactivity-directed separation led to the identification of four compounds, viz. denbinobin (1), 3,7-dihydroxy-2,4-dimethoxyphenanthrene (2), 3-methylgigantol (3), and erianthridin (4) from the ethanolic extract of Ephemerantha lonchophylla. Antiplatelet tests were carried out using 4 different aggregation inducers, viz. arachidonic acid (AA), thrombin, collagen and platelet activating factor (PAF). The results indicated that only compounds 2, 3, and 4 exhibited generally significant anti-aggregation activities with that against AA-induced aggregation being most effective. Estimated IC₅₀, values in this regard for 2, 3, and 4 were 24 μM, 30 μM and 9 μM, respectively.

References

• 1 Hsu  H Y,, Chen  Y P,, Sheu  S J,, Hsu  C S,, Chen  C C,, Chang  H C.. Oriental Materia Medica - a Concise Guide, 1986, p. 582, Oriental Healing Arts Institute, Los Angeles. 
• 2 Ma  G X,, Xu  G J,, Xu  L S,, Wang  Z T.. Microscopic identification of the Chinese drug Shihu IV.  J. of China Pharmaceutical University. 1995;;  26 134-8
• 3 Chen  C C,, Wu  L G,, Ko  F N,, Teng  C N.. Antiplatelet aggregation principles of Dendrobium loddigesii. .  J. Nat. Prod.. 1994;;  57 1271-4
• 4 Chen  H Y,, Shiao  M S,, Huang  Y L,, Shen  C C,, Lin  Y L,, Kuo  Y H et al.. Antioxidant principles from Ephemerantha lonchophylla. .  J. Nat. Prod.. 1999;;  62 1225-7
• 5 Tezuka  Y,, Hirano  H,, Kikuchi  T,, Xu  G J.. Constituents of Ephemerantha lonchophylla, isolation and structure elucidation of new phenolic compounds, ephemeranthol-A, ephemeranthol-B, and ephemeranthoquinone, and of a new diterpene glucoside, ephemeranthoside.  Chem. Pharm. Bull.. 1991;;  39 593-8
• 6 Leong  Y W,, Kang  C C,, Harrison  L J,, Powell  A D.. Phenanthrenes, dihydrophenanthrenes and bibenzyls from the orchid Bulbophyllum vaginatum. .  Phytochemistry. 1997;;  44 157-65
• 7 Vargaftig  B B,, Chignard  M,, Benveniste  J.. Present concepts on the mechanisms of platelet aggregation.  Biochem. Pharmacol.. 1981;;  30 263-71
• 8 Vane  J R.. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs.  Nature-New Biology. 1971;;  231 232-5
• 9 Siess  W.. Molecular mechanisms of platelet activation.  Physiological Review. 1989;;  69 58-178
• 10 Chen  C C,, Huang  Y L,, Ou  J C,, Su  M J,, Yu  S M,, Teng  C M.. Bioactive principles from the roots of Lindera megaphylla. .  Planta Medica. 1991;;  57 406-8
• 11 Born  G VR,, Cross  M J.. The aggregation of blood platelet.  J. Physiol.. 1963;;  168 178-95