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Planta Med 2000; 66(7): 678-681
DOI: 10.1055/s-2000-8635
Letter
Georg Thieme Verlag Stuttgart · New York

On the Stability of Sesquiterpene Lactones in the Officinal Arnica Tincture of the German Pharmacopoeia1

Thomas J. Schmidt2,*, Uwe Matthiesen3 , Günter Willuhn2
  • 1 The authors dedicate this work to Professor Dr. B. C. Lippold, Institut für Pharmazeutische Technologie der Heinrich-Heine-Universität Düsseldorf, on the occasion of his 60th birthday
  • 2 Institut für Pharmazeutische Biologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany
  • 3 Institut für Klinische Chemie und Laboratoriumsdiagnostik, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany
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Publication History

Publication Date:
31 December 2000 (online)

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Abstract

An investigation of sesquiterpene lactone content in Arnica tincture (German Pharmacopoeia) after storage for three years at different temperatures (+ 4, + 25 and + 30 °C) was carried out by GC and GC-MS analysis. A decrease (13, 32 and 37 %, respectively) in the content of the main active compounds in this preparation, 11α,13-dihydrohelenalin esters, correlating with storage temperature was found. This change was shown to be caused by addition of ethanol to the cyclopentenone structure of these molecules leading to 2-ethoxy-2,3,11,13-tetrahydrohelenalin derivatives.

References

  • 1 Willuhn  G.. Arnicae flos. Wichtl M Teedrogen und Phytopharmaka. 3rd edition. Stuttgart:; Wissenschaftliche Verlagsgesellschaft 1997: 78-82
    Google Scholar
  • 2 Willuhn  G,, Leven  W.. Qualität von Arnikazubereitungen - Sesquiterpenlactongehalt von Tinkturen und wässrigem Aufguss.  Deutsche Apotheker Zeitung. 1995;;  135 1939-42
    PubMedGoogle Scholar
  • 3 Schmidt  T J.. Helenanolide type sesquiterpene lactones. III. Rates and stereochemistry in the reaction of helenalin and related helenanolides with sulfhydryl containing biomolecules.  Bioorganic & Medicinal Chemistry. 1997;;  5 645-53
    PubMedGoogle Scholar
  • 4 Willuhn  G,, Röttger  P M,, Wendisch  D.. 6-O-Isobutyryl-tetrahydrohelenalin aus Blüten von Arnica montana. .  Planta Medica. 1984;;  50 35-7
    PubMedGoogle Scholar
  • 5 Willuhn  G,, Skibinski  A,, Schmidt  T J.. Structure revision of xanthalongin and further sesquiterpene lactones from flowers of Arnica longifolia.  Planta Medica. 1998;;  64 635-9
    PubMedGoogle Scholar
  • 6 Schmidt  T J.. Toxic activities of sesquiterpene lactones: Structural and biochemical aspects.  Current Organic Chemistry. 1999;;  3 577-605
    PubMedGoogle Scholar
  • 7 Leven  W,, Willuhn  G.. Sesquiterpene lactones from Arnica chamissonis Less. VI. Identification and quantitative determination by high-performance-liquid and gas chromatography.  Journal of Chromatography. 1987;;  410 329-42
    CrossrefPubMedGoogle Scholar
  • 8 Willuhn  G,, Leven  W.. Zur qualitativen und quantitativen Analyse der Sesquiterpenlactone von Arnikablüten DAB 9.  Pharmazeutische Zeitung Wissenschaft. 1991;;  4/136 32-9
    PubMedGoogle Scholar
  • 9 Willuhn  G,, Leven  W,, Luley  C.. Arnikablüten DAB 10 - Untersuchungen zur qualitativen und quantitativen Variabilität des Sesquiterpenlactongehaltes der offiziellen Arnikablüten.  Deutsche Apotheker Zeitung. 1994;;  134 49-59
    PubMedGoogle Scholar

Dr. Priv.-Doz. Thomas J. Schmidt

Institut für Pharmazeutische Biologie Heinrich-Heine-Universität Düsseldorf

Universitätsstraße 1 (Geb. 26.23)

40225 Düsseldorf

Germany

Email: schmidtt@uni-duesseldorf.de

Phone: +49/(0)211-81-11923