Anti-Hepatitis B Virus Effects of Wogonin Isolated from Scutellaria baicalensis

Abstract

By using an HBV-producing cell line (MS-G2) in vitro culture system, we found that wogonin isolated from Scutellaria baicalensis can suppress HBV surface antigen production (P < 0.001) without evidence of cytotoxicity. By assaying the endogenous HBV DNA polymerase activity, we found that both the relaxed circular and the linear forms of HBV DNA are significantly reduced in the wogonin-treated group. Wogonin deserves to be further evaluated for the treatment of human HBV infection.

Human hepatitis B virus - HBsAg - HBeAg - endogenous polymerase activity - Scutellaria baicalensis - Labiatae - wogonin - baicalein - baicalin

References

• 1 Szmuness  W.. Hepatocellular carcinoma and hepatitis B virus: evidence for a causal association.  Prog. Med. Virol.. 1978;;  24 40-69
• 2 Maupas  P,, Goudeau  A,, Coursaget  P,, Chiron  J P,, Drucker  J,, Barin  F, et al.. Hepatitis B virus infection and primary hepatocellular carcinoma: epidemiological, clinical, and virological studies in Senegal from the perspective of prevention by active immunization. In: Essex M, Todaro G, Zur Hausen H, Eds Viruses in naturally occurring cancer. Cold Spring Harbor:; Cold Spring Harbor Laboratory, 1980;: 481-506
• 3 Beasley  R P,, Hwang  L Y,, Lin  C C,, Chien  C S.. Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22,707 men in Taiwan.  Lancet. 1981;;  2 1129-33
• 4 Blumberg  B S.. Background and perspective. In: Chisari FV, ed Advances in hepatitis research. New York:; Masson 1984;: 1-8
• 5 Mutchnick  M G,, Ehrinpreis  M N,, Kinzie  J L,, Peleman  R R.. Prospectives on the treatment of chronic hepatitis B and chronic hepatitis C with thymic peptides and antiviral agents.  Antiviral Res.. 1994;;  24 245-57
• 6 Wong  D KH,, Cheung  A M,, O’Rourke  K,, Naylor  C D,, Detsky  A S,, Heathcote  J.. Effect of alpha-interferon treatment in patients with hepatitis B e antigen-positive chronic hepatitis B: a meta-analysis.  Ann. Intern. Med.. 1993;;  119 312-23
• 7 Dienstag  J L,, Perrillo  R P,, Schiff  E R,, Bartholomew  M,, Vicary  C,, Rubin  M.. A preliminary trial of lamivudine for chronic hepatitis B infection.  N. Engl. J. Med.. 1995;;  333 1657-61
• 8 Sureau  C,, Romet-Lemonne  J L,, Mullins  J I,, Essex  M.. Production of hepatitis B virus by a differentiated human hepatoma cell line after transfection with cloned circular HBV DNA.  Cell. 1986;;  47 37-47
• 9 Yaginyuma  K,, Shirakata  Y,, Kobayashi  M,, Koike  K.. Hepatitis virus (HBV) particles are produced in a cell culture system by transient expression of transfected HBV DNA.  Proc. Natl. Acad. Sci USA. 1987;;  84 2678-82
• 10 Chang  C,, Jeng  K S,, Hu  C P,, Lo  S J,, Su  T S,, Ting  L P, et al.. Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line.  EMBO J.. 1987;;  6 675-80
• 11 Huang  R L,, Chen  C C,, Huang  Y L,, Hsieh  D J,, Hu  C P,, Chen  C F, et al.. Osthole increases glycosylation of hepatitis B surface antigen and suppresses the secretion of hepatitis B virus in vitro. .  Hepatology. 1996;;  24 508-15
• 12 Liao  J F,, Wang  H H,, Chen  M C,, Chen  C C,, Chen  C F.. Benzodiazepine binding site-interactive flavones from Scutellaria baicalensis root.  Planta Medica. 1998;;  64 571-2
• 13 Tietz  N W.. Fundamentals of clinical chemistry. Philadelphia:; Saunders 1976; p 674
• 14 Junker  M,, Galle  P,, Schaller  H.. Expression and replication of the hepatitis B virus genome under a foreign promoter control.  Nucleic Acids Res.. 1987;;  15 10117-32