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DOI: 10.1055/s-2001-16487
© Georg Thieme Verlag Stuttgart · New York
Inhibitory Activity for Chitin Synthase II from Saccharomyces cerevisiae by Tannins and Related Compounds
Publication History
September 11, 2000
December 15, 2000
Publication Date:
29 April 2004 (online)
![](https://www.thieme-connect.de/media/plantamedica/200106/lookinside/thumbnails/10.1055-s-2001-16487-1.jpg)
Abstract
In the course of search for potent inhibitors of chitin synthase II from natural resources, seven tannins and related compounds were isolated from the aerial part of Euphorbia pekinensis and identified as gallic acid (1), methyl gallate (2), 3-O-galloyl-(-)-shikimic acid (3), corilagin (4), geraniin (5), quercetin-3-O-(2″-O-galloyl)-β-D-glucoside (6), and kaempferol-3-O-(2″-O-galloyl)-β-D-glucoside (7). These and nine related compounds, (-)-quinic acid (8), (-)-shikimic acid (9), ellagic acid (10), kaempferol (11), quercetin (12), quercitrin (13), rutin (14), quercetin-3-O-(2″-O-galloyl)-β-D-rutinoside (15) and 1,3,4,6-tetra-O-galloyl-β-D-glucose (16), were evaluated for the inhibitory activity against chitin synthase II and III. They inhibited chitin synthase II with IC50 values of 18-206 μM, except for two organic acids, (-)-quinic acid (8) and (-)-shikimic acid (9). Among them, 3-O-galloyl-(-)-shikimic acid (3) was the most potent inhibitor against chitin synthase II of Saccharomyces cerevisiae with an IC50 value of 18 μM. The inhibition appears to be selective for chitin synthase II, as they did not appreciably inhibit chitin synthase III.
Key words
Euphorbia pekinensis - Euphorbiaceae - chitin synthase II - chitin synthase III - chitin synthase II inhibitor - tannins - antifungal agents
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Sung-Uk Kim, Ph. D.
Antibiotics Research Laboratory,
Korea Research Institute of
Bioscience and Biotechnology
P. O. Box 115 Yusung,
Taejon 305-333,
Republic of Korea
Fax: +82-42-861-2675
Email: kimsu@mail.kribb.re.kr
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