Planta Med 2001; 67(8): 781-783
DOI: 10.1055/s-2001-18340
Letter

© Georg Thieme Verlag Stuttgart · New York

Application of Sequence Characterized Amplified Region (SCAR) Analysis to Authenticate Panax Species and Their Adulterants

Jun Wang1, 3 , Wai-Yan Ha1 , Fai-Ngor Ngan3 , Paul Pui-Hay But2, 3 , Pang-Chui Shaw1, 3,*
  • 1 Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
  • 2 Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
  • 3 Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
Further Information

Publication History

November 9, 2000

December 17, 2000

Publication Date:
09 November 2001 (online)

Abstract

A 420-bp RAPD fragment from Panax quinquefolius was converted to a sequence characterized amplified region (SCAR) marker. The main difference between the SCAR of P. quinquefolius and its homolog in P. ginseng is the presence of a 25 bp insertion in the latter. Primers derived from this sequence were successfully used to authenticate six Panax species and two common adulterants.

Abbreviations

AP-PCR:arbitrarily-primed polymerase chain reaction

PCR-RFLP:polymerase chain reaction-restriction fragmentlength polymorphism

RAPD:random amplified polymorphic DNA

RAPDAG:the polymorphic band isolated from P. quinquefolius

SCAR:sequence characterized amplified region

References

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Prof. Pang-Chui Shaw

Department of Biochemistry

Chinese University of Hong Kong

Shatin, N.T.

Hong Kong

China

Email: pcshaw@cuhk.edu.hk

Fax: 852-26035123