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Planta Med 2001; 67(8): 705-708
DOI: 10.1055/s-2001-18345
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effect of Magnolol and Honokiol from Magnolia obovata on Human Fibrosarcoma HT-1080 Invasiveness in vitro

Hisamitsu Nagase1,*, Koji Ikeda1 , Yoshimichi Sakai2
  • 1 Department of Hygienics, Gifu Pharmaceutical University, Gifu, Japan
  • 2 Gifu Prefectural Institute of Forest Science, Mino, Gifu, Japan
Further Information

Publication History

October 31, 2000

January 28, 2001

Publication Date:
09 November 2001 (online)

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Abstract

We investigated the inhibitory effect of Magnolia obovata Thunb. bark ethanol extracts on human fibrosarcoma HT-1080 cells invasion in a reconstituted basement membrane [Matrigel (MG)]. We found that the effective components of the bark ethanol extracts were magnolol and honokiol, two biphenyl compounds. The extracts, magnolol and honokiol, did not affect HT-1080 cells adhesion to MG, but did inhibit HT-1080 cells migration at a high concentration (100 μM). HT-1080 cells secrete matrix metalloproteinase (MMP)-9, which degrades the extracellular matrix as a part of the invasive process. Magnolol and honokiol inhibited the activity of MMP-9, which may have been responsible, in part, for the inhibition of tumor cell invasiveness.

Key words

Magnolol - honokiol - Magnolia obovata - Magnoliacea - matrix metalloproteinases - tumor cell invasion

References

  • 1 Block G, Patterson B, Subar A. Nutrit. Fruit, vegetables, and cancer prevention: a review of the epidemiological evidence.  Cancer. 1992;  18 1-29
    PubMedSearch in Google Scholar
  • 2 Gao M, Tajima K, Kuroishi T, Hirose K, Inoue M. Protective effects of raw vegetables and fruit against lung cancer among smokers and ex-smokers: a case-control study in the Tokai area of Japan.  Japanease Journal of Cancer Research. 1993;  84 594-600
    PubMedSearch in Google Scholar
  • 3 Fidler I J. Critical factors in the biology of human cancer metastasis: Twenty-eighth G.H.A. Clowes Memorial Award Lecture.  Cancer Research. 1990;  50 6130-8
    PubMedSearch in Google Scholar
  • 4 Sledge GW J r., Qulali M, Goulet R, Bone E A, Fife R. Effect of matrix metalloproteinase inhibitor batimastat on breast cancer regrowth and metastasis in athymic mice.  Journal of National Cancer Institute. 1995;  87 1546-50
    PubMedSearch in Google Scholar
  • 5 Saiki I, Murata J, Nakajima M, Tokura S, Azuma I. Inhibition by sulfated chitin derivatives of invasion through extracellular matrix and enzymatic degradation by metastatic melanoma cells.  Cancer Research. 1990;  50 3631-7
    PubMedSearch in Google Scholar
  • 6 Nagase H, Sasaki K, Kito H, Haga A, Sato T. Inhibitory effect of delphinidin from Solanum melongena on human fibrosarcoma HT-1080 invasiveness in vitro .  Planta Medica. 1998;  64 216-9
    PubMedSearch in Google Scholar
  • 7 Okada Y, Gonoji Y, Maka K, Tomita K, Nakanishi I, Iwata K. et al . Matrix metalloproteinase 9 (92kDa gelatinase/Type IV collagenase) from human fibrosarcoma cells.  Journal of Biological Chemistry. 1992;  267 21 712-9
    PubMedSearch in Google Scholar
  • 8 Stetler-Stevenson W G. Type IV collagensin tumor invasion and metastasis.  Cancer and Metastasis Reviews. 1990;  9 289-303
    CrossrefPubMedSearch in Google Scholar
  • 9 Nakajima M, Cop A M. Tumor invasion and extracellular matrix degradative enzymes: Regulation of activity by organ factors.  Cancer Biology. 1991;  2 115-27
    PubMedSearch in Google Scholar
  • 10 DeCosse J J. Potential for chemoprevention.  Cancer. 1982;  50 (11Suppl) 2550-3
    PubMedSearch in Google Scholar
  • 11 Menon L G, Kuttan R, Kuttan G. Inhibition of lung metastasis in mice induced by B16F10 melanoma cells by polyphenolic compounds.  Cancer Letter. 1995;  95 221-5
    PubMedSearch in Google Scholar
  • 12 Teng C M, Chen C C, Ko F N, Lee L G, Huang T F, Chen Y P. et al . Two antiplatelet agents from Magnolia officinalis .  Thrombosis Research. 1988;  50 757-765
    CrossrefPubMedSearch in Google Scholar
  • 13 Fujita S, Taira J. Biphenyl compounds are hydroxyl radical scavengers: Their effective inhibition for UV-induced mutation in Salmonella typhimurium TA102.  Free Radical Biology and Medicine. 1994;  17 273-7
    CrossrefPubMedSearch in Google Scholar
  • 14 Teng C M, Yu S M, Chen C C, Huang Y L, Huang T F. EDRF-release and Ca++-channel blockade by magnolol, an antiplatelet agent isolated from Chinese herb Magnolia officinalis, in rat thoracic aorta.  Life Science. 1990;  47 1153-61
    CrossrefPubMedSearch in Google Scholar
  • 15 Wang J P, Ho T F, Chang L C, Chen C C. Anti-inflammatory effect of magnolol, isolated from Magnolia officinalis, on A23187-induced pleurisy in mice.  Journal of Pharmaceutical Pharmacology. 1995;  47 857-60
    PubMedSearch in Google Scholar
  • 16 Liotta L A. Tumor invasion and metastases - Role of the extracellular matrix: Rhoads Memorial Award lecture.  Cancer Research. 1986;  46 1-7
    PubMedSearch in Google Scholar
  • 17 Liotta L A, Stetler-Stevenson W G, Steeg P S. Cancer invasion and metastasis: Positive and negative regulatory elements.  Cancer Investigation. 1991;  9 543-51
    PubMedSearch in Google Scholar
  • 18 Nakajima M, Lotan D, Baig M M, Carralero R M, Wood W R, Hendrix M JC. et al . Inhibition by retinoic acid of type IV collagenolysis and invasion through reconstituted basement membrane by metastatic rat mammary adenocarcinoma cells.  Cancer Research. 1989;  49 1698-06
    PubMedSearch in Google Scholar
  • 19 Ishikawa M, Okada F, Hamada J, Hosokawa, Kobayashi H. Changes in tumorigenic and metastatic properties of tumor cells treated with quercetin or 5-azacytidine.  International Journal of Cancer. 1987;  39 338-42
    PubMedSearch in Google Scholar

Hisamitsu Nagase

Department of Hygienics

Gifu Pharmaceutical University

5-6-1 Mitahora-higashi

Gifu

Japan 502-8585

Email: nagase@gifu-pu.ac.jp

Fax: + 81-58-237-5979

Phone: Tel.: + 81-58-237-8579