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Planta Med 2001; 67(9): 858-862
DOI: 10.1055/s-2001-18849
Original Paper
Analysis
© Georg Thieme Verlag Stuttgart · New York

A Fast and Simple GC MS Method for Lignan Profiling in Anthriscus sylvestris and Biosynthetically Related Plant Species

Albert Koulman1 , Rein Bos1 , Manuel Medarde2 , Niesko Pras1 , Wim J. Quax1
  • 1 Department of Pharmaceutical Biology, GUIDE (Groningen University Institute for Drug Exploration), Groningen, The Netherlands
  • 2 Departamento de Química Farmacéutica, Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain
Further Information

Publication History

December 8, 2000

March 23, 2001

Publication Date:
06 December 2001 (online)

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Abstract

A new GC-MS method for monitoring lignans was developed to study the variation in plants and elucidate the biosynthetic steps. A simple and fast extraction procedure for lyophilised plant material was developed, giving a lignan-rich extract. A GC-MS method was set up using an apolar WCOT fused silica column using a high temperature programme (150 °C to 320 °C at 15 °C min-1). This new GC-MS method gave a clear lignan profile of plant material. It was possible to show the large variation in the concentrations of deoxypodophyllotoxin (DOP), yatein and anhydropodorhizol (AHP) in Anthriscus sylvestris (L.) Hoffm. plants growing on different locations using cinchonidin as an internal standard. In contrast with existing GC methods for lignan analysis no derivatisation is needed. It is also possible to use this method for the detection of different classes of lignans in biosynthetically related plant species.

Key words

Anthriscus sylvestris - Apiaceae - biosynthesis - deoxypodophyllotoxin - GC-MS analysis - lignans

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Dr. Niesko Pras

Department of Pharmaceutical Biology

University of Groningen

A. Deusinglaan 1

9713 AV Groningen

The Netherlands

Email: N.Pras@farm.rug.nl

Fax: +31-50-3633000

Phone: Tel.: +31-50-3633299