Synthesis, Table of Contents REVIEW © Georg Thieme Verlag Stuttgart · New York Chemical Synthesis of ¹³ C and ¹5 N Labeled Nucleosides Irene M. Lagoja, Piet Herdewijn*Katholieke Universiteit Leuven, Laboratory of Medicinal Chemistry Rega Institute for Medical Research, Minderbroedersstraat 10, 3000 Leuven, Belgium Fax: +32(16)337340; e-Mail: Irene.Lagoja@rega.kuleuven.ac.be; e-Mail: Piet.Herdewijn@rega.kuleuven.ac.be; Recommend Article Abstract Buy Article All articles of this category Abstract The chemical synthesis of 13C and 15N labeled nucleosides for site-specific incorporation in RNA or DNA oligonucleotides is herein reviewed. The review covers the following aspects: 1: single- and multiple site chemical 13C and/or 15N labeling of purine bases, 2: single- and multiple site chemical 13C and/or 15N labeling of pyrimidine bases, 3: single- and multiple site chemical 13C labeling of the sugar residues. 1 Introduction 2 Enzymatic Synthesis of 13C/15N Labeled Bases and Nucleic Acids 3 Synthesis of 13C/15N Labeled Purines 3.1 Carbon and Nitrogen Labeling at the Level of the Bases 3.2 Carbon and Nitrogen Labeling at the Nucleoside/Nucleotide Level 4 Synthesis of 13C/15N Labeled Pyrimidines 4.1 Synthesis of 13C/15N Labeled Uracil and Thymidine 4.2 Synthesis of 13C/15N Labeled Cytosine 5 13C Labeling of Sugars 5.1 Uniformly 13C5 Labeling of Ribose 5.2 Selective Carbon Labeling at the Sugar Moiety 6 Conclusions Key words chemical synthesis - labeled nucleosides - 13C/15N labeling of nucleic bases - uniformly 13C labeling of sugars - selective 13C labeling of sugars Full Text References References 1a Saenger W. 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