Antiviral Activity of Seven Iridoids, Three Saikosaponins and One Phenylpropanoid Glycoside Extracted from Bupleurum rigidum and Scrophularia scorodonia

Abstract

As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Five of these compounds showed antiviral activity against VSV. The percentages of cellular viability at the non-toxic limit concentrations of the active compounds were: verbascoside 53.6 % at 500 μg/ml, 8-acetylharpagide 32.1 % at 500 μg/ml, harpagoside 43.3 % at 450 μg/ml, scorodioside 47.8 % at 500 μg/ml and buddlejasaponin IV 56.9 % at 25 μg/ml. Although none of the saikosaponins were active against HSV-1, the iridoid scorodioside showed moderate in vitro anti-HSV-1 activity (30.6 % at 500 μg/ml). However, none of the compounds tested in this survey had any effect against poliovirus.

Iridoids - saikosaponins - verbascoside - Bupleurum rigidum - Umbelliferae - Scrophularia scorodonia - Scrophulariaceae - antiviral activity

References

• 1 Ng T B, Huang B, Fong W P, Yeung H W. Anti-human immunodeficiency virus (anti-HIV) natural products with special emphasis on HIV reverse transcriptase inhibitors.  Life Sciences. 1997;  61 933-49
• 2 Vlietinck A J, De Bruyne T, Apers S, Pieters L A. Plant-derived leading compounds for chemotherapy of human immunodeficiency virus (HIV) infection.  Planta Medica. 1998;  64 97-109
• 3 Utrilla M P, Zarzuelo A, Risco S, Ocete M A, Gimenez J, Gamez M. Isolation of a saikosaponin responsible for the anti-inflammatory activity of Bupleurum gibraltaricum Lam. root extracts.  Phytotheraphy Research. 1991;  5 43-5
• 4 Sanchez-Contreras S, Díaz-Lanza A M, Matellano L F, Bernabe M. Ollivier E, Balansard G, Faure R. A sulfated saponin from Bupleurum rigidum .  Journal of Natural Products. 1998;  61 1383-5
• 5 Hayashi K, Hayashi H, Hiraoka N, Ikeshiro Y. Inhibitory activity of soyasaponin II on virus replication in vitro .  Planta Medica. 1997;  63 102-5
• 6 Sindambiwe J B, Calomme M, Geerts S, Pieters L, Vlietinck A J, Vanden Berghe D A. Evaluation of biological activities of triterpenoid saponins from Maesa lanceolata .  Journal of Natural Products. 1998;  61 585-90
• 7 Ahn B Z, Yoon Y D, Lee Y H, Kim B H, Sok D E. Inhibitory effects of bupleuri radix saponins on adhesion of some solid tumor cells and relation to hemolytic action: screening of 232 herbal drugs for anti-cell adhesion.  Planta Medica. 1998;  64 220-4
• 8 Kinjo J, Imagire M, Uduyama M, Arao T, Nohara T. Structure-hepatoprotective relationships of soyasaponins I-IV having soyasapogenol B as aglycone.  Planta Medica. 1998;  64 233-6
• 9 Bermejo P, Abad M J, Silván A M, Sanz A, Fernández L, Sánchez-Contreras S, Díaz Lanza A M. In vivo and in vitro anti-inflammatory activity of saikosaponins.  Life Sciences. 1998;  63 1147-56
• 10 Miyagoshi M, Amagaya S, Ogihara Y. Chloretic action of iridoid compounds.  Journal of PharmacobioDynamics. 1988;  11 186-90
• 11 Breschi M C, Martinotti E, Catalano S, Flamini G, Morelli I, Pagni A M. Vasoconstrictor activity of 8-acetylharpagide from Ajuga reptans .  Journal of Natural Products. 1992;  55 1145-8
• 12 Kapadia G J, Sharma S C, Tokuda H, Nishino H, Ueda S. Inhibitory effect of iridoids on Epstein-Barr virus activation by a short-term in vitro assay for anti-tumor promoters.  Cancer Letters. 1996;  102 223-6
• 13 Chen J L, Blanc P, Stoddart C A, Bogan M, Rozhon E J, Parkinson N, Ye Z, Cooper R ., Balick M, Nanakorn W, Kernan M R. New iridoids from the medicinal plant Barleria prionitis with potent activity against respiratory syncytial virus.  Journal of Natural Products. 1998;  61 1295-7
• 14 Bermejo P, Díaz A M, Silván A M, De Santos J, Fernández L, Sanz A, Abad M J. Effects of some iridoids from plant origin on arachidonic acid metabolism in cellular systems.  Planta Medica. 2000;  66 324-8
• 15 Fernández-Matellano L, Díaz-Lanza A M, Ollivier E, Faure R, Balansard G. An iridoid diglycoside isolated from Scrophularia scorodonia .  Phytochemistry. 1995;  40 1569-71
• 16 Fernández-Matellano L, Ollivier E, Díaz-Lanza A M, Faure R, Balansard G. Iridoids from Scrophularia scorodonia .  Planta Medica. 1995;  61 63
• 17 De Santos J, Fernández L, Díaz-Lanza A M, Rubio B, Ollivier E, Faure R, Balansard G. Catalpol Glycosides from Scrophularia scorodonia .  Pharmazie. 1998;  53 4
• 18 González M E, Alarcón B, Carrasco L. Polysaccharides as antiviral agents: Antiviral activity of carrageenan.  Antimicrobial Agents and Chemotherapy. 1987;  31 1388-93
• 19 González M E, Martínez-Abarca F, Carrasco L. Flavonoids: Potent inhibitors of poliovirus RNA synthesis.  Antiviral Chemistry and Chemotherapy. 1990;  1 203-9