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Planta Med 2002; 68(2): 152-157
DOI: 10.1055/s-2002-20252
Original Paper
Analysis
© Georg Thieme Verlag Stuttgart · New York

Quantitative Determination of the Dual COX-2/5-LOX Inhibitor Tryptanthrin in Isatis tinctoria by ESI-LC-MS

Henning Danz1 , Dietmar Baumann1 , Matthias Hamburger1
  • 1Institute of Pharmacy, Friedrich-Schiller-University, Jena, Germany
Further Information

Publication History

February 15, 2001

June 10, 2001

Publication Date:
22 February 2002 (online)

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Abstract

Isatis tinctoria L. is an old European and Chinese dye plant and anti-inflammatory herb from which the potent cyclooxygenase-2 and 5-lipoxygenase inhibitor tryptanthrin (1) (indolo-[2,1-b]-quinazoline-6,12-dione) was recently isolated as one of the active principles. An HPLC method for the quantitative analysis of the compound in plant material was developed. Reproducible extraction was achieved by accelerated solvent extraction (ASE). Detection by UV at 254 and 387 nm and by electrospray-MS were compared. The low tryptanthrin content in the herb and possible interferences required isocratic high-performance liquid chromatography coupled with electrospray-MS in single ion mode. More than 70 Isatis samples of different origin were analyzed. The tryptanthrin content in leaf samples varied from 0.56 to 16.74 × 10-3 %.

Key words

Tryptanthrin - Isatis tinctoria - Brassicaceae - cyclooxygenase-2 - 5-lipoxygenase - dual inhibitor - accelerated solvent extraction - ASE - electrospray ionization - HPLC

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Prof. Dr. Matthias Hamburger

Institute of Pharmacy

Friedrich-Schiller-University Jena

Semmelweisstrasse 10

07743 Jena

Germany

Email: B7HAMA@rz.uni-jena.de

Fax: +49-3641 949842