Planta Med 2002; 68(2): 183-186
DOI: 10.1055/s-2002-20254
Letter

© Georg Thieme Verlag Stuttgart · New York

New Lupane Glycosides from Pulsatilla chinensis

Wencai Ye1, 2 , Qingwen Zhang2 , Wendy W. L. Hsiao3 , Shouxun Zhao2 , Chun-Tao Che1, 4
  • 1Department of Chemistry, Hong Kong University of Science and Technology, Hong Kong
  • 2Department of Phytochemistry, China Pharmaceutical University, Nanjing, P. R. China
  • 3Department of Biology, Hong Kong University of Science and Technology, Hong Kong
  • 4School of Chinese Medicine, the Chinese University of Hong Kong, Hong Kong
Further Information

Publication History

February 10, 2001

June 17, 2001

Publication Date:
22 February 2002 (online)

Abstract

Two new lupane glycosides along with five known triterpenoids were isolated from the roots of Pulsatilla chinensis (Ranunculaceae). The structures of the new glycosides were determined to be 3-O-β-D-glucopyranosyl(1→3)-α-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranosyl ester (pulsatilloside D, 6) and 3-O-[β-D-glucopyranosyl(1→4)][α-L-rhamnopyranosyl(1→2)]-α-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-glucopyranosyl ester (pulsatilloside E, 7) by spectroscopic analysis and chemical methods. The compounds were evaluated for cytotoxic activities against K-562 human leukemia and HeLa cells.

References

  • 1 Jiangsu New Medical C ollege. Dictionary of Traditional Chinese Medicine. Shanghai Science and Technology Publication Ltd Shanghai; 1977: pp 704-5
  • 2 Ye W C, Ji N N, Zhao S X, Liu J H, Ye T, Mckervey M A, Stevenson P. Triterpenoids from Pulsatilla chinensis .  Phytochemistry. 1996;  42 799-802
  • 3 Ye W C, He A M, Zhao S X, Che C T. Pulsatilloside C from the roots of Pulsatilla chinensis .  J. Nat.Prod.. 1998;  61 658-9
  • 4 Chen W K, Liu Q, Chen L, Kasai R, Tanaka Q. Saponins of the Chinese drug Bai-Tou-Weng. IV. Structures of anemosides B4 and A3.  Acta Chimica Sinica. 1990;  48 501-5
  • 5 Chen W K, Wang B Y, Lu D Y, Lin Q, Lin L Y. Saponins of the Chinese drug Bai-Tau-Weng. III.  Acta Chimica Sinica. 1983;  41 793-5
  • 6 Shimizu M, Shingyouchi K, Morita N, Kizu H, Tomimori T. Triterpenoid saponins from Pulsatilla cernua Spreng. (I).  Chemical & Pharmaceutical Bulletin. 1978;  26 1666-71
  • 7 Mimaki Y, Kuroda M, Asano T, Sashida Y. Triterpene saponins and lignans from the roots of Pulsatilla chinensis and their cytotoxic activity against HL-60 cells.  J. Nat.Prod.. 1999;  62 1279-83
  • 8 Dole M, Nunez G, Merchant A K, Maybaum J, Rode C K, Bloch C A, Castle V P. Bcl-2 inhibits chemotherapy-induced apoptosis in neuroblastoma.  Cancer Research. 1994;  54 3253-9

Prof. Chun-Tao Che

School of Chinese Medicine

The Chinese University of Hong Kong

Shatin

Hong Kong

Email: chect@cuhk.edu.hk

Fax: +852-2603-7203

Phone: +852-2609-8130