Features of Development of Stevia rebaudiana Shoots Cultivated in the Roller Bioreactor and their Production of Steviol Glycosides

Nikolai Bondarev; Oxana Reshetnyak; Alexander Nosov

doi:10.1055/s-2002-33809

Planta Medica, Table of Contents

Planta Med 2002; 68(8): 759-762
DOI: 10.1055/s-2002-33809

Letter

Features of Development of Stevia rebaudiana Shoots Cultivated in the Roller Bioreactor and their Production of Steviol Glycosides

Nikolai Bondarev¹ , Oxana Reshetnyak¹ , Alexander Nosov¹

¹Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia

Abstract

Growth and development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides (SGs) were investigated. It was found that, owing to the highly favorable conditions of shoot cultivation created in such an apparatus, the intensity of shoot growth and SG production appeared to be 1.5 - 2.0 times higher than those of the shoots grown in tubes. These results indicate the existence of a positive correlation between these two processes. The data obtained suggest that the enhanced SG production is due to the differentiation of chlorenchyma cells and formation of specific subcellular structures for the glycoside to be accumulated.

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Nikolai Bondarev

Timiryazev Institute of Plant Physiology

Russian Academy of Sciences

Botanicheskaya st. 35

Moscow, 127276

Russia