Planta Med 2002; 68(11): 1029-1033
DOI: 10.1055/s-2002-35658
Original Paper
Analysis
© Georg Thieme Verlag Stuttgart · New York

Identification and Determination of Ecdysones and Flavonoids in Serratula strangulata by Micellar Electrokinetic Capillary Chromatography

Shufang Wang1 , Jingqiu Dai2 , Xingguo Chen1 , Zhide Hu1
  • 1Department of Chemistry, Lanzhou University, Lanzhou, People’s Republic of China
  • 2Institute of Organic Chemistry, National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, People’s Republic of China
Further Information

Publication History

Received: March 8, 2002

Accepted: June 8, 2002

Publication Date:
26 November 2002 (online)

Abstract

The simultaneous determination of 20-hydroxyecdysone (1), 25-deoxy-11,20-dihydroxyecdysone (2), kaempferol 4′,7-dimethyl ether (3) and biochanin A (4) in the fruit, leaf and root of Serratula strangulata Iljin has been investigated by micellar electrokinetic capillary chromatography for the first time. With an electrolyte containing 15 mmol/L borate, 60 mmol/L SDS, 20 % (v/v) methanol, at pH 9.08 and 20 kV applied voltage, the four analytes were completely separated within 22.7 min. The effects of concentration of borate, electrolyte pH, the concentrations of SDS and organic modifier and the applied voltage on electrophoretic behavior and separation were studied. Regression equations revealed linear relationships (correlation coefficients: 0.9986 - 0.9997) between the peak areas of the analytes and the corresponding concentrations. The levels of analytes in the different parts of S. strangulata were easily determined with recoveries ranging from 95.3 % to 104.0 %.

References

  • 1 Jiangsu New Medical C ollege. A Dictionary of Chinese Medicinal Material. Vol. 1 Shanghai; Shanghai People’s Press 1977: p. 234-5
  • 2 Dai J Q, Hou Z F, Zhu Q X, Yang L, Li Y. Sesquiterpenes and flavonoids from Serratula strangulata .  J Chin Chem Soc. 2001;  48 249-52
  • 3 Dai J Q. Investigation on the chemical constituents of Serratula strangulata . Master thesis 1999: p. 30-40
  • 4 Wilson I D, Morgan E D, Lafont R, Shockcor J P, Lindon J C, Nicholson J K, et a l. High performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy and mass spectrometry applied to plant products: identification of ecdysteroids from Silene otites .  Chromatographia. 1999;  49 374-8
  • 5 Odinokov V N, Galyautdinov I V, Nedopekin D V, Khalilov L M, Shashkov A S, Kachala V V, et a l. Phytoecdysteroids from the juice of Serratula coronata L. (Asteraceae).  Insect Biochem Mol Biol. 2002;  32 161-5
  • 6 Wang S M, Liang S W, Zhu X C. Measurement of the content of ecdysterone in Pilao’ an capsule by HPLC.  Chin Pharm J. 2000;  35 471-2
  • 7 Yasukawa K, Takido M, Takeuchi M, Nakagawa S. Effect of chemical constituents from plants on 12-O-tetradecanoylphorbol-13-acetate-induce inflammation in mice.  Chem Pharm Bull. 1989;  37 1071-3
  • 8 Louden D, Handley A, Lafont R, Taylor S, Sinclair I, Lenz E. et al . HPLC analysis of ecdysteroids in plant extracts using superheated deuterium oxide with multiple on-line spectroscopic analysis (UV, IR, H-1 NMR, and MS).  Anal Chem. 2002;  74 288-94
  • 9 Maróy P, Vargha J, Horváth K. Molting hormone levels of insects. Part I. Rapid heterologous hapten radioimmunoassay for insect molting hormone.  FEBS Lett. 1997;  81 319-22
  • 10 Hsiao T H, Hsiao C. Ecdysteroids in the ovary and the egg of the greater wax moth.  J Insect Physiol. 1979;  25 45-52
  • 11 Varga E, Szendrei K, Hajdú Z s, Hornok L, Csaki G y. Study of the compounds contained in Hungarian-grown Leuzea carthamoides D.C  Herba Hung. 1986;  25 115-33
  • 12 Lafont R, Pennetier J -L, Andrianjafintrimo M, Claret J, Modde J -F, Blais C. Sample processing for high-performance liquid chromatography of ecdysteroids.  J Chromatogr. 1982;  236 137-49
  • 13 Yasuda A, Ikeda M, Naya Y. Analysis of hemolymph ecdysteroids in the crayfish Procambarus clarkii by high-performance capillary electrophoresis.  Nippon Suisan Gakkaishi. 1993;  59 1793-9
  • 14 Bathori M, Mathe I, Guttman A. Determination of 20-hydroxyecdysone content by thin-layer chromatography and micellar electrokinetic chromatography.  Chromatographia. 1998;  48 145-8
  • 15 Suntornsuk L. Capillary electrophoresis of phytochemical substances.  J Pharm Biomed Anal. 2002;  27 679-98
  • 16 Kreimeyer J, Petereit F, Nahrstedt A. Separations of flavan-3-ols and dimeric proanthocyanidins by capillary electrophoresis.  Planta Med. 1998;  64 63-7
  • 17 Lechtenberg M, Quandt B, Kohlenberg F -J, Nahrstedt A. Qualitative and quantitative micellar electrokinetic chromatography of kavalactones from dry extracts of Piper methysticum Forst. and commercial drugs.  J Chromatogr A. 1999;  848 457-64
  • 18 Zhang Z, Hu Z, Yang G. Identification and determination of aesculin and aesculetin in ash barks by capillary zone electrophoresis.  Chromatographia. 1997;  44 162-8
  • 19 Renou-Gonnord M F, David K. Optimized micellar electrokinetic chromatographic separation of benzodiazepines.  J Chromatogr A. 1996;  735 249-61
  • 20 Weinberger R, Lurie I S. Micellar electrokinetic capillary chromatography of Illicit drug substances.  Anal Chem. 1991;  63 823-7

Prof. Xingguo Chen

Department of Chemistry

Lanzhou University

Lanzhou, 730000

P. R. of China

Email: chenxg@lzu.edu.cn

Fax: +86-931-8912582