RSS-Feed abonnieren
DOI: 10.1055/s-2003-37718
Recent Development of Antitumor Agents from Chinese Herbal Medicines; Part I. Low Molecular Compounds
In memory of Prof. Dr. Dietrich Schmähl, Deutsches Krebsforschungszentrum, Heidelberg
Dr. Barbara Bertram
Toxicology and Cancer Risk Factors
Deutsches Krebsforschungszentrum
Im Neuenheimer Feld 280
69120 Heidelberg
Germany
eMail: b.bertram@dkfz.de
Fax: +49 6221-423031
Publikationsverlauf
Received: May 27, 2002
Accepted: November 16, 2002
Publikationsdatum:
07. März 2003 (online)
- Abstract
- Introduction
- Alkaloids
- 1,4-Benzoquinone Derivatives
- Diterpenes
- Flavones
- Indigoid Bisindoles
- Conclusion
- References
Abstract
A series of low molecular compounds from Chinese herbal medicines which have proved to be, in some cases, highly effective especially in tumor therapy, is listed here (part II will deal with high molecular compounds, to be published in the next issue). In contrast to synthetic agents used in cancer chemotherapy, these natural compounds have relatively low toxicities. Many of the clinical studies referred to in this paper have been carried out on Asians. Because genetic factors influence enzyme levels, sometimes leading to striking differences in metabolism and pharmacokinetics of drugs, results obtained in clinical studies carried out in China are not 100 % transferable to the European population. The mechanisms of action of these compounds are manifold, consisting of reactions with DNA bases, intercalation in DNA, inhibition of topoisomerases, inhibition of protein kinases, induction of apoptosis etc. Some of the compounds have interesting structural features, that may be used as lead structures for the development of further antitumor agents.
Key words
Antitumor agents - Chinese herbal medicines - low molecular natural compounds - traditional Chinese medicine
Introduction
As a consequence of the experience gathered over thousands of years, Chinese herbal medicines are considered as a rich source for the discovery of new drugs. In the search of new therapeutic agents, many compounds with new structural features and mechanisms of action have been isolated from Chinese herbal medicines. In recent years, a number of Chinese herbs and their active principles were reported to be antineoplastic and cytotoxic both in experimental and clinical studies. The cytotoxic and antineoplastic mechanisms of these compounds involve DNA intercalation, inhibition of DNA topoisomerases and protein kinases, induction of apoptosis, covalent binding to enzymes of biological importance and some unknown mechanisms. The natural compounds are relatively non-toxic as compared to some synthetic agents. In the present paper, a summary of the most important findings on cytotoxic and antineoplastic compounds of low molecular weight from Chinese herbal medicines is given in order to contribute to the further development of antitumor drugs.
#Alkaloids
#Phenanthridine Alkaloids
Lycobetaine (ungeremine, AT-1840), a quaternary phenanthridinium alkaloid from some Amaryllidaceous plants, such as Lycoris radiata Herb. [1] was reported to be antineoplastic. In reviews and in meeting reports [2], [3], lycobetaine was mentioned to be active in the treatment of cervical, ovarian, gastric and other cancers in clinical trials. The overall response in 233 cases of different cancers was reported to be about 35 % [3]. No significant myelotoxic, cardiotoxic and hepatotoxic side effects have been observed [2].[*]
In experimental studies, lycobetaine was found to inhibit the growth of S180 [4] and KB [5] tumor cell lines in vitro and to be significantly active against Ehrlich ascites carcinoma, ascites hepatoma, leukemias L1210 and P388, Lewis lung carcinoma and Yoshida ascites sarcoma in mice or in rats by i. p. injection. In nude mice bearing human gastric cancer xenografts, lycobetaine extended the survival time and decreased the tumor size. Moreover, it was shown to have a direct cytocidal effect on stomach cancer cells in vitro even at low concentrations and to arrest carcinoma cells in the G2/M phase as demonstrated by flow cytometry [6].
A study on the interaction of lycobetaine with calf thymus DNA revealed that lycobetaine intercalates with DNA base pairs, especially the GC-pair [7]. Lycobetaine did not bind covalently to DNA [8]. Studies on structure-activity relationships revealed that the calculated interaction energy of lycobetaine analogues with double-stranded oligonucleotides correlated with their anticancer potency [9], [10]. According to the 3-dimensional structure patterns of the drug-oligonucleotide complex, the quaternary nitrogen atom in lycobetaine plays an important role in the formation of hydrogen bonds between the compound and the oligonucleotide [11]. The betaine and a methylenedioxy group in lycobetaine are supposed to be critical for its antitumor activity [2].
Recently, we have found that lycobetaine is toxic for topoisomerases I and II, and that it strongly inhibits the growth of human tumor xenografts in vitro and in vivo. The IC50 values of lycobetaine in the clonogenic assay against 21 human tumor xenografts of various tumor types range from 0.002 to 27.5 μM with a mean IC50 of 0.8 μM. Intraperitoneal administration of lycobetaine at a dose of 30 mg/kg on days 1 - 5 and 8 - 12 to nude mice bearing the large cell lung carcinoma LXFL529 resulted in a significant growth delay of the tumors [12], corresponding to about 40 % of the LD50 by single i. p. injection [13]. Lycobetaine was localized predominately in the nucleus, it competed with ethidium bromide for intercalation into calf thymus DNA and displaced the DNA minor groove binder Hoechst 33 258. At growth inhibitory concentrations, lycobetaine inhibited topoisomerases I and II, stabilized the covalent DNA-topoisomerase I intermediate, the so-called cleavable complex, and induced apoptosis. Dose-dependent induction of DNA strand breaks was detected by single cell gel electrophoresis (comet assay) [12].
Lycobetaine is structurally related to the phenanthridine alkaloid lycorine as a major Amaryllidaceous constituent and can be easily obtained in high yield from this constituent by oxidation with SeO2 [2]. Lycorine was also reported to be cytotoxic [14]. Recently, lycorine has been found to be highly active in inhibiting a number of human tumor cell lines in vitro, including LXFL529L, Molt4, HL60, K562, U937 [15], GXF251L, and CXF94L [16]. In contrast to lycobetaine, lycorine did not compete with ethidium bromide for intercalation into calf thymus DNA and did not displace the DNA minor groove binder Hoechst 33 258, indicating that its cytotoxic mechanism is different from that of lycobetaine [15], [16]. In addition, lycorine was reported to induce flat morphology in K-Ras-transformed fibroblasts in vitro [17].
#Benzo[c]phenanthridine Alkaloids
Among the benzo[c]phenanthridine alkaloids, nitidine, fagaronine and ethoxychelerythrine are the major components in Radix Zanthoxyli (Liangmianzhen), the root of Zanthoxylum nitidum (Roxb.) DC (Rutaceae) [18] which is listed in the Chinese Pharmacopoeia [19]. Chelerythrine, chelidonine and the related benzo[c]phenanthridine alkaloid sanguinarine are known to be the major components in the whole plant of Chelidonium majus L. (Papaveraceae), a medicinal herb used in European countries and in China [20].[*]
Nitidine was reported to possess antineoplastic activity against both L1210 and P388 leukemias, Lewis lung carcinoma, and B16 melanoma in mice [21]. It increased the life span of mice inoculated with Ehrlich ascites tumor, caused a decrease in the mitotic index and size of the tumor cells, and inhibited DNA and RNA synthesis in tumors [22]. Ethoxychelerythrine also showed inhibitory activity against Ehrlich ascites carcinoma cells [23]. Nitidine chloride was noted to be effectively used in the clinical treatment of chronic myelocytic leukemia [24].
Nitidine and fagaronine were reported to bind to calf thymus DNA by intercalation [25] and to be toxic to topoisomerases I and II [26]. Nitidine exhibited strong stabilization of the covalent binary complex formed between topoisomerase I and DNA [18]. Yeast cells expressing human DNA topoisomerase I were specifically sensitive to nitidine [27]. Unlike camptothecin as reference compound, nitidine and fagaronine bound directly to and mediated the unwinding of B form DNA. Inhibition of topoisomerase II by nitidine was observed at the high concentration of 40 μM in comparison to the inhibition of topoisomerase I at 0.15 - 0.3 μM [28].
The antitumor mechanism of fagaronine is also believed to be due to inhibition of topoisomerases [29], [30]. Fagaronine is a DNA major groove intercalator, does not show any sequence specificity of DNA intercalation, but its highly electronegative oxygen of the hydroxy group is shown to be an acceptor of the hydrogen bond of the amino group of guanine in DNA [31]. In contrast, N-demethylfagaronine was neither cytotoxic, nor effectively interacted with DNA, suggesting that the quaternary nitrogen atom is an important prerequisite for its biological efficacy [32].
Chelerythrine was reported to be a specific protein kinase C (PKC) inhibitor [33], [34]. It completely suppressed the growth of GI-101A breast tumor cells stimulated by hydroxychloroquine and prednisone [35], blocked the expression of vascular endothelial growth factor (VEGF) mRNA in GI-101A and HL-60 cells stimulated by 12-O-tetradecanoylphorbol 13-acetate (TPA) or diethylstilbestrol [36], and inhibited the increased proliferation of MCF-7 cells stimulated by thymeleatoxin [37]. Chelerythrine also inhibited the proliferation of the human prostate cancer cell line, PC3 [38], and gastric cancer cell line, AGS [39]. Incubation of AGS cells with chelerythrine resulted in an arrest of cells in the G0/G1 phase, in induction of apoptosis and in an elevation of p53, p21, c-Myc, and Bax [39]. Chelerythrine was further reported to exhibit cytotoxic activity against a series of radioresistant and chemoresistant human squamous cell carcinoma lines. The carcinoma cells undergo apoptosis rapidly after treatment with chelerythrine in vitro. The antitumor effect of chelerythrine was also demonstrated in nude mice against a radioresistant, chemoresistant and p53-deficient human head and neck squamous cell carcinoma line SQ-20B with significant tumor growth delay and minimal toxicity (40). Chelerythrine also inhibited taxol-mediated polymerization of rat brain tubulin and inhibited colchicine and podophyllotoxin binding to tubulin with IC50 values of about 60 μM [41].
Sanguinarine was reported to be a potent inhibitor of the catalytic subunit of rat liver protein kinase A (PKA) with an IC50 of 6 μM, but a relatively poor inhibitor of PKC with an IC50 of greater than 200 μM [42]. Sanguinarine potently inhibited the growth of human keratinocytes with an IC50 of 0.2 μM [43]. It decreased concentration-dependently the viability of human epidermoid carcinoma A431 cells at lower concentrations than of normal human epidermal keratinocytes. Sanguinarine treatment of A431 cells resulted in an induction of apoptosis but did not lead to formation of a DNA ladder in normal keratinocytes, even at higher concentrations [44]. Sanguinarine interacted with calf thymus DNA and altered its secondary structure. The maximum of sanguinarine-binding to DNA in buffer of low ionic strength and acidic pH and the number of bound alkaloid molecules per base pair, at saturation, is higher in G-C rich DNA than in A-T rich DNA [44].
#Protoberberine Alkaloids
Like benzo[c]phenanthridine, protoberberine is also a four-ring system but with one saturated bond. Protoberberine alkaloids occur in numerous plants, including several medicinal plants. Berberine is the most studied protoberberine alkaloid, widely distributed in plants used in traditional Chinese medicine. Plant items listed in the Chinese Pharmacopoeia containing berberine and related protoberberine alkaloids are Rhizoma Coptidis (Huanglian), the rhizome of Coptis chinensis Franch., C. deltoidea C.Y. Cheng et Hsiao, or C. teeta Wall. (Ranunculaceae); Caulis Mahoniae (Ganglaomu), the stem of Mahonia healei (Fort.) Carr. or M. fortunei (Lindl.) Fedde (Berberidaceae) and Cortex Phellodendri (Huangbo), the stem bark of Phellodendron chinense Schneid. or P. amurense Rupr. (Rutaceae).[*]
Berberine was reported to possess significant cytotoxicity against some human cancer cell lines, P388 murine leukemia cells [45], and 9L rat glioma cell line [46]. Recently, Iizuka et al. have reported that the extract of Rhizoma Coptidis and berberine significantly inhibited the proliferation of six esophageal cancer cell lines in vitro in a concentration-dependent manner [47].
Chi et al. reported on a flow-cytometric study of the effect of berberine on human hepatoma HepG2 cells. Continuous exposure of HepG2 cells to berberine resulted in a concentration-dependent growth inhibition of the cells. Berberine treatment caused a significant reduction of the S phase fraction of HepG2 cells [48] and an arrest of gastric cancer cells in the G2/M phase [49]. Esophageal cancer cells treated with the extract of Rhizoma Coptidis showed an accumulation in the G0/G1 phase and a relative decrease of the S phase [47]. Concentration-dependent effects of berberine were also reported on cell cycle and apoptosis in Balb/c 3T3 cells [50]. Chang et al. reported that berberine down-regulated K-Ras2 gene expression associated with morphologic differentiation in human embryonal carcinoma cells [51]. Furthermore, berberine and some related protoberberine alkaloids induced apoptosis in murine thymocytes [52] and in promyelocytic leukemia HL60 cells [53]. Berberine was also found to be a DNA triplex binder [54] and to enhance the cytotoxicity of clinically used antitumor agents, such as nucleotides [55] and nitrosoureas [56] in vitro. It was also reported that berberine up-regulated the multidrug-resistant transporter (pgp-170) expression in two oral (KB, OC2), two gastric (SC-M1, NUGC-3) and two colon (COLO 205, CT 26) cancer cell lines [57], [58].
The artifact berberrubine, formed by demethylation of berberine, was found to be highly cytotoxic against P388 leukemia, L1210 leukemia, B16 melanoma and some human cancer cell lines [59]. Makhey et al. reported that berberrubine functions as a potent inhibitor of topoisomerase II in vitro [60]. The protoberberine alkaloids epiberberine and groenlandicine were identified as topoisomerase I inhibitors in vitro [61]. Topoisomerase II-mediated DNA cleavage assays showed that berberrubine poisons the enzyme by stabilizing the enzyme-DNA complexes. Berberrubine induced DNA cleavage in a site-specific and concentration-dependent manner. Comparison of the cleavage pattern of berberrubine with that of etoposide revealed that berberrubine represents a new class of antitumor agent with topoisomerase II poisoning activity as well as catalytic inhibition. The step at which berberrubine induces a cleavable complex is possibly different from that of etoposide [62].
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 1 Lycobetaine and lycorine.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 2 Nitidine, fagaronine, and chelerythrine.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 3 Berberine, epiberberine, berberrubine, and groenlandicine.
1,4-Benzoquinone Derivatives
From the seed of Iris pallasii var. chinensis (Iridaceae), a 1,4-benzoquinone derivative with an unsaturated aliphatic side chain (irisquinone A), has been isolated [63]. The seed of I. pallasii var. chinensis is used in Chinese folk medicine as a fertility regulating agent and for the treatment of malignant diseases.[]
Irisquinone A was found to be cytotoxic and antitumorigenic. In mice tumor xenografts, it showed growth inhibitory activity against cervical cancer U14 and Ehrlich carcinoma by i. p. application and against lymphosarcoma by i. p. and oral administration [64]. Oral treatment of mice bearing U14 tumor with irisquinone A at a dosage of 100 mg/kg or i. v. treatment at a dosage of 5 mg/kg once every other day for 5 cycles, starting 24 h after implantation of the tumor, caused a tumor inhibition rate of 35 - 55 % [65]. The LD50 of irisquinone A in mice was about 28 mg/kg after i. p. and about 2.8 g/kg after oral administration. The chemotherapeutic indices (LD50/ED50) of irisquinone A were estimated to be 5 by i. p. administration and 14 by oral administration.
Radiosensitizing effects of irisquinone A were found in vitro against the tumor cell lines U14 [64], S-180V [65], HeLa [66], against Ma 7373 breast cancer cells in mice, and against human intestinal mucoadenocarcinoma in nude mice [66]. The mechanism of this effect was considered to be an inhibition of oxygen consumption and depletion of glutathione in tumor cells [66]. In a clinical trial, irisquinone A, given orally to 558 patients with cancer of the lung and esophagus, or with superficial metastatic cancer during radiotherapy, significantly contributed to the reduction of tumor size and to the prolongation of survival time of the patients [64].
Related 1,4-benzoquinone derivatives were isolated from the Chinese medicinal plant Ardisia japonica (Thunb.) Bl. (Myrsinaceae). One of them is embelin, a 2,5-dihydroxy-1,4-benzoquinone with a saturated side chain. Embelin was found to exhibit significant antitumor activity in rats against autochthoneous fibrosarcomas induced by methylcholanthrene and it prolonged the survival time of the animals [67]. An in vitro study with embelin using a fibrosarcoma cell line showed a concentration-dependent decrease in thymidine uptake and glutathione levels of the tumor cells [68].[*]
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 4 Irisquinone A and embelin.
Diterpenes
#Rabdosia diterpenes
Several Rabdosia species (Lamiaceae) native to China are used in folk medicine as antitumor or anti-inflammatory agents. Studies on the chemical constituents of the leaves and stems and on their biological activities have been carried out with various Rabdosia species, especially Rabdosia rubescens (Hemsl.) Hara. Diterpene compounds, such as oridonin and ponicidin, are derived from kaurane. The diterpenes were found to be the cytotoxic principles in Rabdosia species [69].[]
115 patients suffering from inoperable esophageal carcinoma were treated with chemotherapy alone (group A) or with chemotherapy plus R. rubescens (group B); in group A, 10 out of 31 patients (32.3 %) treated with chemotherapy alone, responded to the treatment, including 2 partial responses (greater than 50 % tumor regression) and 8 minimal responses. In group B, 59 out of 84 patients (70.2 %) responded to the treatment, including 10 complete responses (100 % tumor regression), 16 partial and 33 minimal responses. The one-year survival rates of group A and group B were 13.6 % and 41.3 %, respectively. No significant differences were observed between the two groups regarding the side effects alopecia, anorexia, nausea and hyperpyrexia which occurred in more than 30 % of patients [70]. Between August 1974 and January 1987, 650 patients with moderate and advanced esophageal carcinoma were treated with a combination of chemotherapy and R. rubescens or R. rubescens plus different traditional Chinese patent medicines. Forty patients survived for over 5 years (5-year survival rate 6.15 %); 32 for over 6 years; 23 for more than 10 years, and 5 for more than 15 years [71]. PC-SPES, a combination of eight herbs including R. rubescens has been tested for the treatment of prostate cancer showing significantly decreased serum prostate specific antigen (PSA) of the patients [72], [73]. In a review, it was mentioned that oridonin and ponicidin were tested in clinical trials for the treatment of esophageal cancer [74].
Oridonin was reported to be cytotoxic against Ehrlich ascites carcinoma [75] and L1210 leukemia [76] in mice after i. p. injection. The cell killing rates of oridonin (15 mg/kg i. p.) on day 5 and day 8 in L1210 cells were 73 % and 39 %. The G2 and S phases of L1210 cells were prolonged, while the G1 phase was unchanged [76]. In in vitro investigations, oridonin exhibited inhibitory effects on the proliferation of the human gastric adenocarcinoma cell line (MGc80 - 3) and esophageal cancer cell line (CaEs-17) at concentrations below 15 μg/ml [77]. An extract of Rabdosia rubescens exerted a cytotoxic effect on cisplatin-sensitive human ovarian cancer A2780 cells with an IC50 of about 0.6 mg/mL [78]. Oridonin was found to inhibit DNA, RNA, and protein syntheses in L1210 cells in vitro in a concentration-dependent manner. Inhibition of DNA and RNA synthesis was fast but reversible, whereas the inhibition of protein synthesis was strong and long lasting [79]. Oridonin inhibited DNA synthesis also in a cell-free system [80].
Oridonin (7.5 mg/kg for 7 days) and cisplatin (0.4 mg/kg for 4 days) showed synergistic antineoplastic effects in mice bearing Ehrlich ascites carcinoma, S180 or P388 leukemia and there was a distinct growth inhibition of Ehrlich ascites carcinoma cells in vitro. In the S180 cell culture, the IC50 values of cisplatin were reduced to 1/3.4 and 1/6.7 by cotreatment with oridonin at concentrations of 0.5 and 1 μg/mL. A greater amount of DNA cross-links and DNA-protein cross-links in S180 cells was detected when the cells were treated with cisplatin plus oridonin instead of cisplatin alone [81]. Synergistic antitumor effects of oridonin with bleomycin A5 were also reported [82]. Several diterpenes which are structurally related to oridonin, were also reported to exert cytotoxic and antitumor activities [83], [84].
The mechanism of oridonin activity was postulated to be due to covalent binding of oridonin to a specific site of enzymes in tumor cells [85]. Oridonin and related diterpenes bearing an α-methylenecyclopentanone structure are electrophilic and can be considered as weak alkylating agents [86]. The reactivity of the α-methylene group was examined using the reaction of oridonin with adenosine and cytidine as nucleic acid model compounds and with thiols, L-cysteine, L-lysine and L-serine as model compounds representing relevant constituents of active centers of enzymes. The reaction with thiols proceeded easily under mild conditions to give the corresponding thioether adducts, the adduct with l-cysteine being formed nearly quantitatively. However, oridonin did not react with adenosine and cytidine under the same conditions [87].
#Quassinoids
Fructus Bruceae (Yadanzi), the mature fruit of Brucea javanica (L.) Merr (Simaroubaceae), is listed in the Chinese Phamacopoeia and is used as an antimalarial and antidysenteric agent and for the treatment of warts and corns by external application. A number of diterpene quassinoids characterized by an epoxy binding between positions 13 and 20 and a hydroxy function at position 5, were isolated from the fruits of B. javanica. Bruceantin and brusatol are two important representatives of these constituents possessing antileukemic activity. Structurally, bruceantin and brusatol differ from each other only in the acid moiety [88].[*]
Kupchan et al. have discovered the antileukemic activity of bruceantin [89]. Bruceantin exerted growth inhibitory effects in vitro and in vivo against a series of tumor cell lines but did not show significant effects in clinical studies against solid tumors [90], [91], [92].
In contrast, an oil emulsion of the fruits of B. javanica was recently reported to show clinical efficacy. From 35 patients with recurrent metastatic gastrointestinal cancers treated with the oil emulsion, 24 patients (46 %) showed a reduction of tumor size and improvement of clinical symptoms [93]. A clinical trial on 68 patients with brain metastasis in lung cancer treated with 10 % oil emulsion combined with radiotherapy resulted in an improvement of living quality and in prolongation of median survival time (15 months) compared to radiotherapy alone (10 months) [94]. Clinical i. v. application of the oil emulsion was reported to improve the intracranial hypertension caused by brain metastasis from lung cancer [95].
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 5 Oridonin and ponicidin.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 6 Bruceantin and brusatol.
Flavones
#Scutellaria Flavones
Radix Scutellariae (Huangqin), the root of Scutellaria baicalensis Georgi (Lamiaceae), is known to contain a number of flavones, namely baicalein, wogonin and the corresponding 7-O-β-D-glucopyranosiduronides baicalin and wogonosides [96], [97]. It is listed in the Chinese Pharmacopeia and is a well known traditional Chinese medicine.[]
Baicalin and baicalein were reported to exhibit growth inhibitory activity towards the human hepatoma cell lines PLC/PRF/5 and Hep-G2 as well as the human pancreatic cancer cell line BxPC-3 with an IC50 of 20 μg/mL for baicalin and 50 μg/mL for baicalein [98]. Cytotoxic effects of baicalein, baicalin, and wogonin were also observed on the human bladder cancer cell lines KU-1 and EJ-1, and on a murine bladder cancer cell line (MBT-2). All three substances inhibited cell proliferation in vitro in a dose-dependent manner with baicalin as the most active compound. An in vivo study showed that the root extract of S. baicalensis at an oral daily dose of 10 mg per mouse for 10 days significantly inhibited the growth of MBT-2 cells implanted to C3H/HeN mice [99]. We have also found that baicalein, baicalin, wogonin, wogonoside and skullcapflavone II (neobaicalein) inhibited the growth of the human tumor cell lines LXFL 529L (large cell lung carcinoma) and HL60 at a micromolar range [100].
Baicalein strongly inhibited DNA topoisomerase II, probably by stabilizing the covalent enzyme-DNA intermediate in a ternary complex. It also inhibited the proliferation of three human hepatocellular carcinoma cell lines [101]. A structure-activity analysis revealed that flavones with hydroxy groups at the 5, 7, 3′ and 4′ positions favored topoisomerase II-mediated DNA cleavage. Recently, we have found that baicalein also inhibits topoisomerase I by stabilizing the otherwise cleavable complex [100]. Formation of the cleavable complex might contribute to the growth inhibitory activity of flavones on human tumor cell lines [102]. Baicalin was also found to inhibit cell proliferation and to induce apoptosis in several human prostate cancer cell lines [103].
Baicalin, wogonin, skullcapflavone II and wogonoside exhibited only weak inhibitory properties on tyrosine kinase activity of the epidermal growth factor receptor (EGFR) with IC50 values exceeding 60 μM. In contrast, we found that baicalein is a potent inhibitor of EGFR with an IC50 of 1.1 μM. In A431 cells overexpressing EGFR, baicalein was significantly more active than in other cell lines [100]. Baicalein strongly inhibited human T-lymphoid leukemia cell proliferation with an IC50 of about 5 μM. Protein tyrosine kinase activity in human T-lymphoid leukemia cells was significantly reduced by baicalein [104].
Moreover, baicalein has been reported to inhibit the activity of cAMP phosphodiesterases (PDE). We confirmed the PDE inhibitory activity of baicalein and further showed that baicalein is able to inhibit the cAMP-specific isoenzyme family PDE4 with an IC50 of 10 μM. The respective glucuronide baicalin is only a weak PDE4 inhibitor. However, when LXFL 529L whole cells were incubated with baicalein, no inhibition of the intracellular PDE activity was observed and the intracellular cAMP level remained unchanged [100].
Baicalein potently inhibited the growth of a human breast carcinoma cell line, MDA-MB-435 with an IC50 of about 6 μg/mL and was more active than flavones isolated from citrus fruits including hesperetin and naringenin [105]. Baicalein and related flavones also inhibited the proliferation of estrogen receptor-positive MCF-7 human breast cancer cells; the inhibition was not reversible by an addition of estrogen [106]. Baicalein and wogonin strongly inhibited xanthine oxidase, indicating that they might be useful for the remission of brain tumors, since xanthine oxidase serum levels are increased in tissues of brain tumors [107]. Baicalein as an α-glucosidase inhibitor suppressed in vitro invasion and in vivo metastasis of mouse melanoma cells [108].
#Polymethoxylated Flavones
Fructus Aurantii (Zhiqiao) and Fructus Aurantii immaturus (Zhishi), the immature fruits of Citrus aurantium L. (Rutaceae) and its cultivated variants are listed in the Chinese Pharmacopoeia. They contain polymethoxylated flavones such as tangeretin, nobiletin, sinensetin and auranetin.[]
The polymethoxyflavones from C. aurantium were found to possess a wide spectrum of biological activities. Tangeretin, nobiletin and related polymethoxyflavones were reported to exert antiproliferative activities against a number of tumor cell lines [109] and to induce differentiation of HL60 cells in vitro in a concentration-dependent manner [110]. HL60 cells treated with these flavones differentiated into mature monocyte/macrophage. A structure-activity study revealed that the ortho-catechol moiety in the 2-phenyl nucleus of flavones is a prerequisite for their antiproliferative activity and for the induction of differentiation in HL60 cells. A hydroxy group at C3 and a methoxyl group at C8 enhanced the activity [111]. Tangeretin and nobiletin also inhibited the invasion of mouse MO4 cells into embryonic chick heart fragments in vitro [112].
An ethyl acetate extract of orange juice did not affect the initial uptake rate of [3 H]vinblastine by Caco-2 cells but significantly increased the steady-state uptake of cyclosporin A as a P-glycoprotein inhibitor. 3,3′,4′,5,6,7,8-Heptamethoxyflavone, tangeretin and nobiletin were found to be active compounds. They all increased the steady-state uptake of [3 H]vinblastine by Caco-2 cells in a concentration-dependent manner. The ethyl acetate extract and the polymethoxyflavones also increased steady-state [3 H]vinblastine uptake by LLC-GA5-COL300 cells, a cell line transfected with human MDR1 cDNA [113]. The ethyl acetate extract of grapefruit juice, orange juice and the polymethoxyflavones was also reported to increase the uptake of [3 H]vincristine into adriamycin-resistant human myelogenous leukemia K562/ADM cells [114].
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 7 Baicalein, wogonin, and skullcapflavone II.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 8 Tangeretin, nobiletin, sinensetin, and auranetin.
Indigoid Bisindoles
In the 1960’s, Chinese scientists began to study the active principle in the Chinese patent medicine ”Danggui Longwei Wan” which had been effectively used as an antileukemic agent [115]. This official patent medicine contains 11 ingredients, including Indigo naturalis. The antileukemic activity was found to be associated with indirubin, a minor component in Indigo naturalis [116]. Indirubin is a bisindole derivative with a 3,2′-linkage, while indigo and isoindigo bisindoles possess 2,2′- and 3,3′-linkages, respectively. In experimental studies, indirubin inhibited Lewis lung carcinoma in mice and W256 sarcoma in rats [117].[*]
Treatment of 314 patients suffering from chronic myelocytic leukemia (CML) with indirubin at daily oral doses of 300 - 450 mg per patient resulted in 82 complete remissions (26 %), 38 partial remissions (12 %) and 87 beneficial effects (28 %). The overall response was 87 % [118]. In a further study, treatment of CML patients with indirubin for 1.5 - 6 months markedly increased 5′-nucleotidase activity of leukocytes in some cases with a palliative effect [119]. In another clinical trial including 57 cases of CML, treatment with indirubin resulted in a median survival of 31.5 months without obvious side effects [120].
Because of the manifold findings on antileukemic effects of indirubin, different studies were carried out on its mechanisms of action. Indirubin turned out to inhibit cell-free DNA synthesis catalyzed by partially purified DNA-dependent DNA polymerase from Ehrlich ascites tumor cells in a concentration-dependent manner. This inhibition could not be reversed by increasing the DNA concentration, but could be abolished by increasing the enzyme concentration. The strongest inhibition resulted after preincubation with the enzyme and DNA, suggesting that indirubin, DNA and the enzyme formed a tertiary complex [121]. The DNA polymerase 1 activities of chronic myelocytic leukemia cells from the peripheral blood of patients decreased significantly after treatment with indirubin. Similar results have been observed with Escherichia coli DNA polymerase 1 in vitro [122].
Intraperitoneal or s. c. administrations of indirubin at a daily dose of 200 mg/kg partially inhibited the incorporation of [3 H]thymidine into the DNA of W256 sarcoma cells but not the incorporation of [3 H]uridine into RNA and of [14 C]phenylalanine into protein [123]. [3 H]Thymidine incorporation into DNA of liver and spleen of healthy control mice was not affected by i. p. injection of indirubin, whereas hepatic and splenic [3 H]thymidine incorporation was inhibited in mice bearing L7212 leukemia [124]. After incubation of indirubin with calf thymus DNA, the λmax 207 nm of indirubin shifted towards a longer wavelength with decreasing absorbance. The binding of indirubin to DNA was rather weak, since indirubin molecules were easily released during precipitation with alcohol or gel filtration. The amount of bound [3 H]indirubin was concentration dependent. Calf thymus DNA bound 46 indirubin molecules per 1000 nucleotides [125].
Electron microscopic examination of peripheral blood samples and leukocytes of bone marrow from patients with chronic granulocytic leukemia treated with indirubin showed a swelling of the cell nucleus membrane, swelling and degeneration of the rough endoplasmic reticulum, and condensation of chromosomes [126]. Measuring the labelling index of bone marrow cells from patients with chronic myelocytic leukemia in vitro using [3 H]thymidine before and after indirubin treatment revealed a decrease of labelling index, the decrease being most significant in myelocytes and polychromatophilic erythroblasts [127].
Electron spin resonance spectrometry demonstrated that the C:C double bond obviously plays an important role in the anticancer activity of indirubin and indigo [128]. Recently, we have found that indirubin and its analogues are potent inhibitors of cyclin-dependent kinases (CDKs). The crystal structure of CDK2 in a complex with indirubin derivatives showed that indirubin interacts with the kinase’s ATP-binding site through van der Waals interactions and three hydrogen bonds [129], [130], [131]. In fact, a number of structures closely related to indirubin were reported to be cyclin-dependent kinase inhibitors [132]. Cell cycle molecular targets are being used for the development of new anticancer drugs [133].
Subacute toxicity testing of indirubin administered orally at 100 - 400 mg/kg per day to rats for 30 days showed no effects on leukocytes or on liver or renal function. Oral application of 80 mg/kg of indirubin per day for 1 - 3 months to rats caused anorexia and diarrhea, but no effects on bone marrow. Long-term administration of high doses of indirubin did not affect the hematopoietic stem cell production or DNA formation [134]. A subacute toxicity study of indirubin in dogs receiving three different doses showed no adverse effects at a low daily dosage (20 mg/kg). At a middle daily dosage of 100 mg/kg, mild diarrhea occurred within 10 - 30 days. Serum glutamic-pyruvic transaminase (GPT) levels were slightly elevated after 5 months in one of three dogs treated. At a high daily dosage (200 mg/kg), serious diarrhea and hematofecia occurred during 40 - 60 days and serum GPT levels were elevated after 3 months of treatment. Pathological changes were also observed in all tissue sections obtained after 6 months [135]. Bone marrow and blood indices, renal function, and electrocardiogram were not affected by any of the three dosages [136]. Therefore, indirubin was believed to be safe at clinical daily doses of 150 - 200 mg. The slight liver toxicity observed in dogs receiving 100 mg/kg corresponds to a daily dose of 2240 mg in humans [135]. In human peripheral lymphocytes [137] and in bone marrow cells of patients with chronic myelocytic leukemia [138], [139], indirubin did not increase sister chromatid exchange (SCE).
Several N-substituted indirubin derivatives [140], [141], indirubine acyl derivatives, indirubin oximes [142], and halogenated indirubins [143] were synthesized and tested as potentially antineoplastic agents. Some halogen-substituted indirubine derivatives were reported to exhibit higher antitumor activity against L7212 in mice and W256 sarcoma in rats than native indirubin. The most active compound was found to be 5-iodoindirubine which was active against W256 sarcoma, L7212, L7712, L759 leukemias, and Lewis lung carcinoma. It showed increases in life-span of mice bearing L7212 leukemia by 41 - 73 %, and of rats bearing W256 sarcoma by 48 - 83 %. The antitumor activity of 5-iodoindirubin against W256 sarcoma and L7212 leukemia was significantly higher than that of indirubin at the same dosage. In vitro, the IC50 value of 5-iodoindirubin on [3 H]thymidine incorporation into DNA of P388 cells was 6.4 μg/mL compared to 17 μg/mL of indirubin [143], [144].
Bisindole derivatives with 2,2′-, 3,3′- or 3,2′-linkages were compared for their effects on nucleic acid and protein synthesis. They all formed complexes with calf thymus DNA as measured by UV and visible spectrometry and inhibited nucleic acid and protein synthesis on cultured W256 sarcoma cells and in vivo. Isoindigo derivatives showed the highest potency in inhibiting DNA synthesis in cancer cells and in cell-free systems, whereas indigo derivatives had the lowest activity [145], [146]. N-Methylisoindigo (meisoindigo) was more potent than indirubin against W256 sarcoma in rats [143]. It was suggested that the improved absorption of meisoindigo is one of the major reasons for the enhanced antitumor activity as compared to indirubin. Studies on the mechanism of action of N-methylisoindigo indicated that it strongly inhibited DNA biosynthesis in L1210 cells and caused an arrest of S phase cells [147]. At a non-toxic concentration of 0.7 μg/mL N-methylisoindigo induced differentiation of ML-1 human myeloblastic leukemic cells as the most pronounced effect accompanied by the down-regulation of c-Myb gene expression, suggesting that the antitumor mechanisms of N-methylisoindigo involve these effects. N-Methylisoindigo was reported to be a second generation derivative, and was chosen for clinical treatment of chronic myeloid leukemia [148].[*]
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 9 Indirubin, indigo, and isoindigo.
Conclusion
A series of low molecular compounds from Chinese herbal medicines, which proved to be in some cases highly effective especially in tumor therapy, has been listed here (part II will deal with high molecular compounds, to be published the next issue). These medicinal plants have been used since hundreds of years in China. In contrast to synthetic agents used in cancer chemotherapy, natural compounds have relatively low toxicities. Obviously, because of genetic factors that influence enzyme levels accounting for sometimes striking differences in metabolism and pharmacokinetics of drugs, results obtained in clinical studies carried out in China are not transferable to 100 % to the European population. Since many of the clinical studies referred to in this paper have been carried out on Asians; the outcome of such studies in Caucasians or Africans might not be the same.
The mechanisms of action of these compounds are manifold, consisting in reactions with DNA bases, intercalation in DNA, inhibition of topoisomerases, inhibition of protein kinases, induction of apoptosis, etc. Some of the compounds presented here have interesting structural features, which have been or can be used as lead structures for the development of further antitumor agents.
#References
- 1 Owen T Y, Wang S Y, Chang S Y, Lu F L, Yang C L, Hsu B. A new antitumor substance - lycobetaine (AT-1840). Ko Hsueh Tung Pao. 1976; 21 285-7
- 2 He H M, Weng Z Y. Structure-activity relationship study of the new anticancer drug lycobetaine (AT-1840). Acta Pharmaceutica Sinica. 1989; 24 302-4
- 3 Xu B. Pharmacologic study of several anticancer agents of natural origin. Proceedings of Intern Summit on Drugs from Natural Products. October 9 - 12, 1995 Beijing, China; Vol 1
- 4 Ghosal S, Singh S K, Kumar Y, Unnikrishnan S, Chattopadhyay S. Chemical constituents of Amaryllidaceae. XXVI. The role of ungeremine in the growth-inhibiting and cytotoxic effects of lycorine: evidence and speculation. Planta Medica. 1988; 54 114-6
- 5 Wang X W, Yu W J, Shen Z M, Yang J L, Xu B. Cytotoxicity of hydroxycamptothecin and four other antineoplastic agents on KB cells. Acta Pharmacologica Sinica. 1987; 8 86-90
- 6 Wu Y L, Wu Y X, Yu C S, Zhang S Y, Su Z C, Jiang S J. The cytocidal effect of AT-1840 and parvovirus H-1 on gastric cancer cells. Shanghai Medical Journal. 1988; 11 683-8
- 7 Liu J, Yang S L, Xu B. Characteristics of the interaction of lycobetaine with DNA. Acta Pharmacologica Sinica. 1989; 10 437-42
- 8 Liu J, Yang S L, Xu B. Effects of lycobetaine on chromatin structure and activity of murine hepatoma cells. Science in China Series B. 1990; 33 1459-65
- 9 Chen J Z, Chen K X, Jiang H L, Lin M W, Ji R Y. Theoretical investigation on interaction binding of analogs of AT-1840 to double-stranded polynucleotide. Progress in Natural Science. 1997; 7 329-35
- 10 Wang P, Shi G B, Song G Q, Chen K X, Ji R Y. Assignment of proton resonances and conformational characterization of oligodeoxyribonucleic acid d(CCGTACGG) in solution. Acta Biochimica and Biophysica Sinica. 1996; 28 703-5
- 11 Gan L, Liu X J, Chen K L, Ji Y Y. Computer simulation on the interaction between antitumor agent lycobetaine and DNA. Gaojishu Tongxun. 1992; 2 30-3
- 12 Barthelems H U, Niederberger E, Roth T, Schulte K, Tang W C, Zankl H, Fiebig H H, Eisenbrand G, Marko D. Lycobetaine acts as a selective topoisomerase IIβ poison and inhibits the growth of human tumor cells. British Journal of Cancer. 2001; 85 1585-91
- 13 Zhang S Y, Lu F L, Yang J L, Wang L J, Xu B. Effect on animal tumors and toxicity of lycobetaine acetate. Acta Pharmacologica Sinica. 1981; 2 41-5
- 14 Hua D H, Saha S, Takemoto D J. Anticancer activities of 2,5,8,9-substituted 6-oxo-1,2,3,4,5,6-hexahydrophenanthridines on multi-drug-resistant phenotype cells. Anticancer Research. 1997; 17A 2435-41
- 15 Niederberger E. Mechanismus-orientierte Untersuchungen zur antineoplastischen Wirkung von Naturstoffen und Naturstoff-Derivaten. Thesis, Universtiy of Kaiserslautern Germany; 1998
- 16 Schulte K. Synthese von Phenanthridin- und Benzo[c]phenanthridin-Derivaten und Untersuchungen auf ihre biologische Wirkung. Thesis, Universtiy of Kaiserslautern Germany; 2000
- 17 Kushida N, Atsumi S, Koyano T, Umezawa K. Induction of flat morphology in K-ras-transformed fibroblasts by lycorine, an alkaloid isolated from the tropical plant Eucharis grandiflora . Drugs under Experimental and Clinical Research. 1997; 23 151-5
- 18 Fang S D, Wang L K, Hecht S M. Inhibitors of DNA topoisomerase I isolated from the roots of Zanthoxylum nitidum . Journal of Organic Chemistry. 1993; 58 5025-7
- 19 The Pharmacopoeia of the People’s Republic of China. Vol I Ed 2000 Chemical Industrial Press Beijing, PR China;
- 20 Colombo M L, Bosisio E. Pharmacological activities of Chelidonium majus L. (Papaveraceae). Pharmacological Research. 1996; 33 127-34
- 21 Stermitz F R, Gillespie J P, Amoros L G, Romero R, Stermitz T A, Larson K A, Earl S, Ogg J E. Synthesis and biological activity of some antitumor benzophenanthridinium salts. Journal of Medicinal Chemistry. 1975; 18 708-13
- 22 Fan Y J, Zhou J, Li M. Effect of nitidine chloride on the life cycle of Ehrlich ascites carcinoma cells in mice. Acta Pharmacologica Sinica. 1981; 2 46-9
- 23 Wang M H. Isolation of antitumor alkaloids from Zanthoxylum nitidum and structural study of its alkaloid C. Chinese Pharmaceutical Bulletin. 1981; 16 48
- 24 Zhu D Y. Recent advances on the active components in Chinese medicines. Abstracts of Chinese Medicines (Hong Kong). 1987; 1 251-286
- 25 Kubova N, Smekal E, Kleinwachter V, Cushman M. Binding properties of nitidine and its indenoisoquinoline analog with DNA. Studia Biophysica. 1986; 114 251-6
- 26 Gatto B, Sanders M M, Yu C, Wu H Y, Makhey D, LaVoie E J, Liu L F. Identification of topoisomerase I as the cytotoxic target of the protoberberine alkaloid coralyne. Cancer Research. 1996; 56 2795-800
- 27 Del Poeta M, Chen S F, Von Hoff D, Dykstra C C, Wani M C, Manikumar G, Heitman J, Wall M E, Perfect J R. Comparison of in vitro activities of camptothecin and nitidine derivatives against fungal and cancer cells. Antimicrobial Agents and Chemotherapy. 1999; 43 2862-78
- 28 Wang X, Henningfeld K A, Hecht S M. DNA topoisomerase I-mediated formation of structurally modified DNA duplexes. Effects of metal ions and topoisomerase I inhibitors. Biochemistry. 1998; 37 2691-700
- 29 Chen Y Z, Tang G Y, Xu B J, Wu Q J, Lu C Z, Li J Q, Huang Z X. The formation and crystal structure of dihydronitidine and discussion of anticancer mechanism of nitidine cation. Science in China Series B. 1992; 35 1101-9
- 30 Larsen A K, Grondard L, Couprie J, Desoize B, Comoe L, Jardillier J C, Riou J F. The antileukemic alkaloid fagaronine is an inhibitor of DNA topoisomerases I and II. Biochemical Pharmacology. 1993; 46 1403-12
- 31 Fleury F, Sukhanova A, Ianoul A, Devy J, Kudelina I, Duval O, Alix A J, Jardillier J C, Nabiev I. Molecular determinants of site-specific inhibition of human DNA topoisomerase I by fagaronine and ethoxidine. Relation to DNA binding. Journal of Biological Chemistry. 2000; 275 3501-9
- 32 Pezzuto J M, Antosiak S K, Messmer W M, Slaytor M B, Honig G R. Interaction of the antileukemic alkaloid, 2-hydroxy-3,8,9-trimethoxy-5-methylbenzo[c]phenanthridine (fagaronine), with nucleic acids. Chemico-Biological Interactions. 1983; 43 323-39
- 33 Herbert J M, Augereau J M, Gleye J, Maffrand J P. Chelerythrine is a potent and specific inhibitor of protein kinase C. Biochemical and Biophysical Research Communications. 1990; 172 993-9
- 34 Sampaio-Maia B, Soares-da-Silva P. Ca2+/calmodulin mediated pathways regulate the uptake of l-DOPA in mouse neuroblastoma neuro 2A cells. Life Sciences. 2000; 67 3209-20
- 35 Fernandez Y, Ramakrishnan R, Rathinavelu A. GI-101A breast tumor cell growth. Life Sciences. 2000; 67 567-75
- 36 Ramakrishnan R, Zell J A, Malave A, Rathinavelu A. Expression of vascular endothelial growth factor mRNA in GI-101A and HL-60 cell lines. Biochemical and Biophysical Research Communications. 2000; 270 709-13
- 37 Mueller H, Liu R, David F, Eppenberger U. Selective modulation of protein kinase A and protein kinase C activities in epidermal growth factor (EGF)-stimulated MCF-7 breast cancer cells. Biological Chemistry. 1997; 378 1023-9
- 38 Lamm M L, Long D D, Goodwin S M, Lee C. Transforming growth factor-β1 inhibits membrane association of protein kinase Cα in a human prostate cancer cell line, PC3. Endocrinology. 1997; 138 4657-64
- 39 Zhu G H, Wong B C, Eggo M C, Yuen S T, Lai K C, Lam S K. Pharmacological inhibition of protein kinase C activity could induce apoptosis in gastric cancer cells by differential regulation of apoptosis-related genes. Digestive Diseases and Sciences. 1999; 44 2020-6
- 40 Chmura S J, Dolan M E, Cha A, Mauceri H J, Kufe D W, Weichselbaum R R. In vitro and in vivo activity of protein kinase C inhibitor chelerythrine chloride induces tumor cell toxicity and growth delay in vivo . Clinical Cancer Research. 2000; 6 737-42
- 41 Wolff J, Knipling L. Antimicrotubule properties of benzophenanthridine alkaloids. Biochemistry. 1993; 32 13 334-9
- 42 Wang B H, Lu Z X, Polya G M. Inhibition of eukaryote protein kinases by isoquinoline and oxazine alkaloids. Planta Medica. 1997; 63 494-8
- 43 Vavreckova C, Gawlik I, Muller K. Benzophenanthridine alkaloids of Chelidonium majus. II. Potent inhibitory action against the growth of human keratinocytes. Planta Medica. 1996; 62 491-4
- 44 Ahmad N, Gupta S, Husain M M, Heiskanen K M, Mukhtar H. Differential antiproliferative and apoptotic response of sanguinarine for cancer cells versus normal cells. Clinical Cancer Research. 2000; 6 1524-8
- 45 Dai J R, Chai H, Pezzuto J M, Kinghorn A D, Tsauri S, Padmawinata K. Studies on Indonesian Plants. V. Cytotoxic constituents of the roots of the Indonesian medicinal plant Fibraurea chloroleuca . Phytotherapy Research. 1993; 7 290-4
- 46 Chen K T, Hao D M, Liu Z X, Chen Y C, You Z S. Effect of berberine alone or in combination with argon ion laser treatment on the 9L rat glioma cell line. Chinese Medicinal Journal (Engl). 1994; 107 808-12
- 47 Iizuka N, Miyamoto K, Okita K, Tangoku A, Hayashi H, Yosino S, Abe T, Morioka T, Hazama S, Oka M. Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Letters. 2000; 148 19-25
- 48 Chi C W, Chang Y F, Chao T W, Chiang S H, Peng F K, Lui W Y, Liu T Y. Flow-cytometric analysis of the effect of berberine on the expression of glucocorticoid receptors in human hepatoma HepG2 cells. Life Sciences. 1994; 54 2099-2107
- 49 Lin H L, Chang Y F, Liu T Y, Wu C W, Chi C W. Submicromolar paclitaxel induces apoptosis in human gastric cancer cells at early G1 phase. Anticancer Research. 1998; 18 3443-9
- 50 Yang I W, Chou C C, Yung B Y. Dose-dependent effects of berberine on cell cycle pause and apoptosis in Balb/c 3T3 cells. Naunyn-Schmiedebergs Archives of Pharmacology. 1996; 354 102-8
- 51 Chang K S, Gao C, Wang L C. Berberine-induced morphologic differentiation and down-regulation of c-Ki-ras2 protooncogene expression in human teratocarcinoma cells. Cancer Letters. 1990; 55 103-8
- 52 Miura N, Yamamoto M, Ueki T, Kitani T, Fukuda K, Komatsu Y. Inhibition of thymocyte apoptosis by berberine. Biochemical Pharmacology. 1997; 53 1315-22
- 53 Kuo C L, Chou C C, Yung B Y. Berberine complexes with DNA in the berberine-induced apoptosis in human leukemic HL-60 cells. Cancer Letters. 1995; 93 193-200
- 54 Ren J, Chaires J B. Sequence and structural selectivity of nucleic acid binding ligands. Biochemistry. 1999; 38 16 067-75
- 55 Lee S J, Kim J B, Lee S W, Kim J H. Enhanced cytotoxicity of berberine and some anticancer nucleotides against tumor cell-lines. Archives of Pharmacal Research. 1995; 18 138-9
- 56 Zhang R X, Dougherty D V, Rosenblum M L. Laboratory studies of berberine used alone and in combination with 1,3-bis(2-chloroethyl)-1-nitrosourea to treat malignant brain tumors. Chinese Medicinal Journal (English Edition). 1990; 103 658-65
- 57 Lin H L, Liu T Y, Wu C W, Chi C W. Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel. British Journal of Cancer. 1999; 81 416-22
- 58 Lin H L, Liu T Y, Lui W Y, Chi C W. Up-regulation of multidrug resistance transporter expression by berberine in human and murine hepatoma cells. Cancer. 1999; 85 1937-42
- 59 Dai J R, Chai H, Pezzuto J M, Kinghorn A D, Tsauri S, Padmawinata K. Studies on Indonesian Plants. V. Cytotoxic constituents of the roots of the Indonesian medicinal plant Fibraurea chloroleuca . Phytotherapy Research. 1993; 7 290-4
- 60 Makhey D, Gatto B, Yu C, Liu A, Liu L F, LaVoie E J. Protoberberine alkaloids and related compounds as dual inhibitors of mammalian topoisomerase I and II. Medicinal Chemistry Research. 1995; 5 1-12
- 61 Kobayashi Y, Yamashita Y, Fuji N, Takaboshi K, Kawakami T, Kawamura M, Mizukami T, Nakano H. Inhibitors of DNA topoisomerase I and II isolated from the Coptis rhizomes . Planta Medica. 1995; 61 414-8
- 62 Kim S A, Kwon Y, Kim J H, Muller M T, Chung I K. Induction of topoisomerase II-mediated DNA cleavage by a protoberberine alkaloid, berberrubine. Biochemistry. 1998; 37 16 316-24
- 63 Xia G C, Zang J Y, Lu X M, Xiao P G. Resource utilization and herbal study of ”malinzi” (Iris lactea Pall. var. chinensis). Acta Pharmacentica Sinica. 1985; 20 316-9
- 64 Li D H, Hao X G, Zhang S K, Wang S X, Liu R Y, Ma K S, Yu S P, Jiang H, Guan J F. Antitumor effect and toxicity of irisquinone. Acta Pharmacologica Sinica. 1981; 2 131-4
- 65 Wang S X, Liu M F, Yuan L, Zhang W L, Li D H. Effects of irisquinone on cyclic nucleotides in plasma, cancer and other tissues of mice bearing U14 tumor. Chinese Journal of Clinical Oncology. 1986; 13 241-3
- 66 Li W M, Wang S H, Kuang P, Qu B X, Song H L, Zhu L X. Radiosensitizing effect of an active principle Iq7611 from the seed of Iris lactea var. chinenesis . Tumor. 1987; 7 97-9
- 67 Chitra M, Sukumar E, Suja V, Devi C S. Antitumor, anti-inflammatory and analgesic property of embelin, a plant product. Chemotherapy. 1994; 40 109-113
- 68 Chitra M, Sukumar E, Devi C S. [3 H]-Thymidine uptake and lipid peroxidation by tumor cells on embelin treatment: an in vitro study. Oncology. 1995; 52 66-8
- 69 Yuan K, Hu R, Ji C, Yin M. New method for preparing oridonin by column chromatography. China Journal of Chinese Materia Medica. 1997; 22 478-80, 511
- 70 Wang R L, Gao B L, Xiong M L, Mei Q D, Fan K S, Zuo Z K, Lang T L, Gao G Q, Ji Z C, Wei D C. Potentiation by Rabdosia rubescens on chemotherapy of advanced esophageal carcinoma. Chinese Journal of Oncology. 1986; 8 297-9
- 71 Wang R L. A report of 40 cases of esophageal carcinoma surviving for more than 5 years after treatment with drugs. Chinese Journal of Oncology. 1993; 15 300-2
- 72 Porterfield H. UsToo PC-SPES surveys: review of studies and update of previous survey results. Molecular Urology. 2000; 4 289-91
- 73 de la Taille A, Hayek O R, Burchardt M, Burchardt T, Katz A E. Role of herbal compounds (PC-SPES) in hormone-refractory prostate cancer: two case reports. Journal of Alternative and Complementary Medicine. 2000; 6 449-51
- 74 Lou F C, Ding L S, Ma Q Y, Du F L. Natural antineoplastic compounds and their structure-activity relationships. Journal of Nanjing College of Pharmacy. 1986; 17 152-9
- 75 Fujita T, Takeda Y, Sun H D, Minami Y, Marunaka T, Takeda S, Yamada Y, Togo T. Cytotoxic and antitumor activities of Rabdosia diterpenoids. Planta Medica. 1988; 54 414-7
- 76 Wang M Y, Lin C, Zhang T M. Cytokinetic effects of oridonin on leukemia L1210 cells. Acta Pharmacologica Sinica. 1985; 6 195-8
- 77 Li X T, Lin C, Li P Y. Comparison of in vitro assays for the cytotoxic effect of anticancer drugs. Chinese Journal of Oncology. 1986; 8 184-6
- 78 Yu J J, Reed E. Preliminary study of the effect of selected Chinese natural drugs on human ovarian cancer cells. Oncology Reporter. 1995; 2 571-5
- 79 Wang M Y, Lin C, Zhang T M. Autoradiographic study on the effects of oridonin on DNA, RNA and protein synthesis of leukemia L 1210 cells. Acta Pharmacologica Sinica. 1987; 8 164-5
- 80 Li Y, Zhang T M. Effect of oridonin on cell-free DNA synthesis in vitro . Acta Pharmacologica Sinica. 1988; 9 465-7
- 81 Gao Z G, Ye Q X, Zhang T M. Synergistic effect of oridonin and cisplatin on cytotoxicity and DNA cross-link against mouse sarcoma S180 cells in culture. Acta Pharmacologica Sinica. 1993; 14 561-4
- 82 Zhang T M, Shou M G, Wang M Y. Antitumor effects of different combinations of oridonin, bleomycin A5 and nitrocaphane. Acta Pharmacologica Sinica. 1986; 7 457-60
- 83 Jiang B, Lu Z Q, Hou A J, Zhao Q S, Sun H D. ent-Kaurane diterpenoids from Isodon lungshengensis . Journal of Natural Products. 1999; 62 941-5
- 84 Sun H D, Lin Z W, Niu F D, Lin L Z, Chai H B, Pezzuto J M, Cordell G A. Cytotoxic ent-kaurene diterpenoids from three Isodon species. Phytochemistry. 1995; 38 437-42
- 85 Fujita E, Nagao y, Node M, Kaneko K, Nakazawa S, Kuroda H. Antitumor activity of the Isodon diterpenoids; structural requirements for the activity. Experientia. 1976; 32 203-6
- 86 Zhai J K, Han W C, Ju X H. Crystal structure of nervosin and the electronic structure of its anticancer-active zone. Acta Chimica Sinica. 1993; 51 854-9
- 87 Fujita E, Nagao Y, Kaneko K, Nakazawa S, Kuroda H. The antitumor and antibacterial activity of the Isodon diterpenoids. Chemical and Pharmaceutical Bulletin (Tokyo). 1976; 24 2118-27
- 88 Hall I H, Lee K H, Okano M, Sims D, Ibuka T, Liou Y F, Imakura Y. Antitumor agents. XLII. Comparison of antileukemic activity of helenalin, brusatol and bruceantin and their esters on different strains of P388 lymphocytic leukemic cells. Journal of Pharmaceutical Sciences. 1981; 70 1147-50
- 89 Liao L L, Kupchan S M, Horwith S B. Mode of action of the antitumor compound bruceantin, an inhibitor of protein synthesis. Molecular Pharmacology. 1976; 12 167-76
- 90 Garnick M B, Blum R H, Canellos G P, Mayer R J, Parker L, Skarin A T, Li F P, Henderson I C, Frei E. Phase I trial of bruceantin. Cancer Treatment Reports. 1979; 63 1929-32
- 91 Amato D A, Borden E C, Shiraki M, Enterline H AT, Rosenbaum C, Davis H L, Paul A R, Stevens C M, Lerner H J. Evaluation of bleomycin, chlorozotocin, MGBG, and bruceantin in patients with advanced soft tissue sarcoma, bone sarcoma, or mesothelioma. Investigational New Drugs. 1985; 3 397-401
- 92 Wiseman C L, Yap H Y, Bedikian A Y, Bodey G P, Blumenschein G R. Phase II trial of bruceantin in metastatic breast carcinoma. American Journal of Clinical Oncology. 1982; 5 389-91
- 93 Cheng J H, Long H, Zu J L. Therapeutic analysis of treatment of recurrent metastatic gastrointestinal carcinomas in 35 patients with Brucea javanica emulsion ”Yandanziyou”. Chinese Traditional Patent Medicine. 1991; 13 21-2
- 94 Wang Z Q. Combined therapy of brain metastasis in lung cancer. Chinese Journal of Integrated Traditional and Western Medicine. 1992; 12 609-10
- 95 Lu J B, Shu S Y, Cai J Q. Experimental study on effect of Brucea javanica oil emulsion on rabbit intracranial pressure. Chinese Journal of Integrated Traditional and Western Medicine. 1994; 14 610-11
- 96 Wang J Z, Chen D Y, Su Y Y. Analytical study on processing of Scutellaria baicalensis Georgi by HPLC. China Journal of Chinese Materia Medica. 1994; 19 340-1
- 97 Tomimori T, Miyaichi Y, Kizu H. On the flavonoid constituents from the roots of Scutellaria baicalensis Georgi. I. Yakugaku Zasshi. 1982; 102 388-91
- 98 Motoo Y, Sawabu N. Antitumor effects of saikosaponins, baicalin and baicalein on human hepatoma cell lines. Cancer Letters. 1994; 86 91-5
- 99 Ikemoto S, Sugimura K, Yoshida N, Yasumoto R, Wada S, Yamamoto K, Kishimoto T. Antitumor effects of scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines. Urology. 2000; 55 951-5
-
100 Niederberger E, Meiers S, Genzlinger A, Zankl H, Tang W C, Eisenbrand G, Marko D. Flavones and inhibition of tumor cell growth: new aspects on the mechanism of action. In: Eisenbrand G, Dayan AD, Elias PS, Grunow W, Schlatter J, editors
Carcinogenic and anticarcinogenic factors in food . Wiley-VCH Weinheim; 1998: 512-3 - 101 Matsuzaki Y, Kurokawa N, Terai S, Matsumura Y, Kobayashi N, Okita K. Cell death induced by baicalein in human hepatocellular carcinoma cell lines. Japanese Journal of Cancer Research. 1996; 87 170-7
- 102 Austin C A, Patel S, Ono K, Nakane H, Fisher L M. Site-specific DNA cleavage by mammalian DNA topoisomerase II induced by novel flavone and catechin derivatives. Biochemical Journal. 1992; 282 883-9
- 103 Chan F L, Choi H L, Chen Z Y, Chan P S, Huang Y. Induction of apoptosis in prostate cancer cell lines by a flavonoid, baicalin. Cancer Letters. 2000; 160 219-28
- 104 Huang H C, Hsieh L M, Chen H W, Lin Y S, Chen J S. Effects of baicalein and esculetin on transduction signals and growth factors expression in T-lymphoid leukemia cells. European Journal of Pharmacology. 1994; 268 73-8
- 105 So F V, Guthrie N, Chambers A F, Moussa M, Carroll K K. Inhibition of human breast cancer cell proliferation and delay of mammary tumorigenesis by flavonoids and Citrus juices. Nutrition and Cancer. 1996; 26 167-81
- 106 So F V, Guthrie N, Chambers A F, Carroll K K. Inhibition of proliferation of estrogen receptor-positive MCF-7 human breast cancer cells by flavonoids in the presence and absence of excess estrogen. Cancer Letters. 1997; 112 127-33
- 107 Chang W S, Lee Y J, Lu F J, Chiang H C. Inhibitory effects of flavonoids on xanthine oxidase. Anticancer Research. 1993; 13 2165-70
- 108 Umezawa K. Inhibition of experimental metastasis by enzyme inhibitors from microorganisms and plants. Advances in Enzyme Regulation. 1996; 36 267-81
- 109 Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. Antiproliferative activity of flavonoids on several cancer cell lines. Bioscience, Biotechnology and Biochemistry. 1999; 63 896-9
- 110 Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. Effect of Citrus flavonoids on HL-60 cell differentiation. Anticancer Research. 1999; 19(A) 1261-9
- 111 Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. HL-60 differentiating activity and flavonoid content of the readily extractable fraction prepared from Citrus juices. Journal of Agricultural and Food Chemistry. 1999; 47 128-35
- 112 Bracke M, Vyncke B, Opdenakker G, Foidart J M, De Pestel G, Mareel M. Effect of catechins and citrus flavonoids on invasion in vitro . Clinical and Experimental Metastasis. 1991; 9 13-25
- 113 Takanaga H, Ohnishi A, Yamada S, Matsuo H, Morimoto S, Shoyama Y, Ohtani H, Sawada Y. Polymethoxylated flavones in orange juice are inhibitors of P-glycoprotein but not cytochrome P450 3A4. Journal of Pharmacology and Experimental Therapeutics. 2000; 293 230-6
- 114 Ikegawa T, Ushigome F, Koyabu N, Morimoto S, Shoyama Y, Naito M, Tsuruo T, Ohtani H, Sawada Y. Inhibition of P-glycoprotein by orange juice components, polymethoxyflavones in adriamycin-resistant human myelogenous leukemia (K562/ADM) cells. Cancer Letters. 2000; 160 21-8
- 115 Institute of H aematology, Chinese Academy of Medical S ciences. Clinical studies of Dang Gui Lu Hui Wan in the treatment of CML. Chinese Journal of Internal Medicine. 1979; 15 86-8
- 116 Wu L M, Yang Y P, Zhu Z H. Studies on the active principles of Indigofera tinctoriain the treatment of CML. Comm Chinese Herb Med. 1979; 9 6-8
- 117 Ji X J, Zhang F R, Lei J L, Xu Y T. Studies on the antineoplastic effect and toxicity of synthetic indirubin. Acta Pharmacentica Sinica. 1981; 16 146-8
- 118 Indirubin cooperative g roup. Clinical study of indirubin in the treatment of 314 patients with chronic granulocytic leukemia. Chinese Journal of Hematology. 1980; 1 132
- 119 Gan W J, Yang T Y, Wen S D, Liu Y Y, Tan Z, Deng C A, Wu J X, Liu M P. Studies on the mechanism of indirubin action in treatment of chronic myelocytic leukemia (CML). II. 5′-Nucleotidase in the peripheral white blood cells of CML. Chinese Journal of Hematology. 1985; 6 611-3
- 120 Qian L S, Xue Y P, Zhang X M, Yang T Y, Yan W W, Wang Z C. Observation on the long period effect of indirubin in the treatment of 57 cases of chronic myelogenous leukemia. Chinese Journal of Hematology. 1991; 12 125-7
- 121 Zhang L, Wu G Y, Qiu C C. Effect of indirubin on DNA synthesis in vitro . Acta Academiae Medicinae Sinicae. 1985; 7 112-6
- 122 Gan W J, Yang T Y, Wang Z C, Qian L S, Ma J, Ge Y Q, Cheng B J, Li Z M, Bo H Q. Studies on the antitumor mechanism of indirubin by treatment of chronic myelocytic leukemia (CML). Chinese Biochemical Journal. 1987; 3 225-30
- 123 Du D J, Ceng Q T. Effect of indirubin on the incorporation of isotope labeled precursors into nucleic acid and protein of tumor tissues. Chinese Traditional and Herbal Drugs. 1981; 12 406-9
- 124 Du D G, Ceng Q T, Wen Z S, Wan X P. Study on the incorporation of 3H-TdR into DNA of liver and spleen of L7212, a lymphatic leukemia in mice. Chinese Journal of Hematology. 1982; 3 277-80
- 125 Wu G Y, Liu J Z, Fang F D, Zuo J. Studies on the mechanism of indirubin action in the treatment of chronic granulocytic leukemia. V. Binding between indirubin and DNA and identification of the type of binding. Scientia Sinica. 1982; 25B 1071-9
- 126 Lee K, Shih C Y, Yang T Y, Chen L S, Chao W M, Sun C S, Wang T C, Pien S K, Sing K H. Ultratherapeutic effect of indirubin for human chronic granulocytic leukemia. National Medical Journal of China. 1979; 59 129-32
- 127 You Y C, Mi J X, Wan J H, Yang T Y, Wang Z C, Qian L S. Indirubin in the treatment of chronic myelocytic leukemia (CML) estimation of labelling index of bone marrow cells by 3H-TdR. Chinese Journal of Oncology. 1987; 9 418-20
- 128 Chen K Y, Wang Z F, Li C Y, Li W X. Study on indirubin and indigo by ESR spectrometry. Chemical Journal of Chinese Universities. 1989; 10 869-71
- 129 Hoessel R, Leclerc S, Endicott J A, Nobel M E, Lawrie A, Tunnah P, Leost M, Damiens E, Marie D, Marko D, Niederberger E, Tang W, Eisenbrand G, Meijer L. Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases. Nature Cell Biology. 1999; 1 60-7
- 130 Marko D, Schatzle S, Friedel A, Genzlinger A, Zankl H, Meijer L, Eisenbrand G. Inhibition of cyclin-dependent kinase 1 (CDK1) by indirubin derivatives in human tumour cells. British Journal of Cancer. 2001; 84 283-9
- 131 Leclerc S, Garnier M, Hoessel R, Marko D, Bibb J A, Snyder G L, Greengard P, Biernat J, Wu Y Z, Mandelkow E M, Eisenbrand G, Meijer L. Indirubins inhibit glycogen synthase kinase-3β and CDK5/p25, two protein kinases involved in abnormal tau phosphorylation in Alzheimer’s disease. A property common to most cyclin-dependent kinase inhibitors?. Journal of Biological Chemistry. 2001; 276 251-60
- 132 Sielecki T M, Boylan J F, Benfield P A, Trainor G L. Cyclin-dependent kinase inhibitors: useful targets in cell cycle regulation. Journal of Medicinal Chemistry. 2000; 43 1-18
- 133 Buolamwini J K. Cell cycle molecular targets in novel anticancer drug discovery. Current Pharmaceutical Design. 2000; 6 379-92
- 134 Wang J H, You Y C, Mi J X, Ying H G. Effect of indirubin on hematopoietic cell production. Acta Pharmacologica Sinica. 1981; 2 241-4
- 135 Wen Z J, Liu Y, Yi Z P, Yang Y S. Effects of indirubin on the histology and histochemistry of canine and rat livers. Bulletin of Chinese Materia Medica. 1988; 13 306-7
- 136 Sichnan Institute of Traditional Chinese M edicine. Subacute toxicity of indirubin in dogs. Chinese Traditional and Herbal Drugs. 1981; 12 27-9
- 137 Cai Y Y, Xu C L, Li S H, Liu Y. Studies on sister chromatid exchanges induced by harringtonine, indirubin and pyquiton before or after activation with microsome enzyme. Acta Academiae Medicinae Sinicae. 1983; 5 161-4
- 138 Feng B Z, Zhang Y H, Qian L S, Chu Y L. Effect of indirubin on SCE frequencies of BM cells in chronic myeloid leukemia. Acta Academiae Medicinae Sinicae. 1984; 6 308-10
- 139 Feng B Z. Sister chromatid exchange frequency of bone marrow cells and its response to indirubin in chronic myeloid leukemia. Chinese Journal of Oncology. 1984; 6 357-60
- 140 Wu K M, Zhang M Y, Fang Z, Huang L. Synthesis of N 1-substituted derivatives of indirubin, an antileukemic compound. Acta Pharmacentica Sinica. 1984; 19 513-8
- 141 Ji XJ Zhang F R. Antineoplastic effect of indirubin derivatives and their structure-activity relationship. Acta Pharmacentica Sinica. 1985; 20 137-9
- 142 Zeng Q T, Du D J, Xie D C, Wang X P, Rau C Q. Antitumor activities of indirubin derivatives. Chinese Traditional and Herbal Drugs. 1982; 13 24-30
- 143 Gu Y C, Li G L, Yang Y P, Fu J P, Li C Z. Synthesis of some halogenated indirubin derivatives. Acta Pharmacentica Sinica. 1989; 24 629-32
- 144 Zeng Q T, Du D J, Si X F, Ran C Q, Wu X P. Antitumor activity of 5′-iodoindirubin. West China Pharm Sci. 1991; 6 63-8
- 145 Wu G Y, Liu J Z, Zhang L. Effect of bisindole compounds with three different kinds of linkage on the synthesis of nucleic acid and protein. Progress in Biochemistry and Biophysics. 1985; 61 48-51
- 146 Wu K M, Zhang M Y, Fang Z, Huang L. Potential antileukemic agents, synthesis of derivatives of indirubin, indigo, and isoindigotin. Acta Pharmacentica Sinica. 1985; 20 821-6
- 147 Ji X J, Liu X M, Li K, Chen R H, Wang L G. Pharmacological studies of meisoindigo: absorption and mechanism of action. Biomedical and Environmental Sciences. 1991; 4 332-7
- 148 Liu X M, Wang L G, Li H Y, Ji X J. Induction of differentiation and down-regulation of c-myb gene expression in ML-1 human myeloblastic leukemia cells by the clinically effective anti-leukemia agent meisoindigo. Biochemical Pharmacology. 1996; 51 1545-51
Dr. Barbara Bertram
Toxicology and Cancer Risk Factors
Deutsches Krebsforschungszentrum
Im Neuenheimer Feld 280
69120 Heidelberg
Germany
eMail: b.bertram@dkfz.de
Fax: +49 6221-423031
References
- 1 Owen T Y, Wang S Y, Chang S Y, Lu F L, Yang C L, Hsu B. A new antitumor substance - lycobetaine (AT-1840). Ko Hsueh Tung Pao. 1976; 21 285-7
- 2 He H M, Weng Z Y. Structure-activity relationship study of the new anticancer drug lycobetaine (AT-1840). Acta Pharmaceutica Sinica. 1989; 24 302-4
- 3 Xu B. Pharmacologic study of several anticancer agents of natural origin. Proceedings of Intern Summit on Drugs from Natural Products. October 9 - 12, 1995 Beijing, China; Vol 1
- 4 Ghosal S, Singh S K, Kumar Y, Unnikrishnan S, Chattopadhyay S. Chemical constituents of Amaryllidaceae. XXVI. The role of ungeremine in the growth-inhibiting and cytotoxic effects of lycorine: evidence and speculation. Planta Medica. 1988; 54 114-6
- 5 Wang X W, Yu W J, Shen Z M, Yang J L, Xu B. Cytotoxicity of hydroxycamptothecin and four other antineoplastic agents on KB cells. Acta Pharmacologica Sinica. 1987; 8 86-90
- 6 Wu Y L, Wu Y X, Yu C S, Zhang S Y, Su Z C, Jiang S J. The cytocidal effect of AT-1840 and parvovirus H-1 on gastric cancer cells. Shanghai Medical Journal. 1988; 11 683-8
- 7 Liu J, Yang S L, Xu B. Characteristics of the interaction of lycobetaine with DNA. Acta Pharmacologica Sinica. 1989; 10 437-42
- 8 Liu J, Yang S L, Xu B. Effects of lycobetaine on chromatin structure and activity of murine hepatoma cells. Science in China Series B. 1990; 33 1459-65
- 9 Chen J Z, Chen K X, Jiang H L, Lin M W, Ji R Y. Theoretical investigation on interaction binding of analogs of AT-1840 to double-stranded polynucleotide. Progress in Natural Science. 1997; 7 329-35
- 10 Wang P, Shi G B, Song G Q, Chen K X, Ji R Y. Assignment of proton resonances and conformational characterization of oligodeoxyribonucleic acid d(CCGTACGG) in solution. Acta Biochimica and Biophysica Sinica. 1996; 28 703-5
- 11 Gan L, Liu X J, Chen K L, Ji Y Y. Computer simulation on the interaction between antitumor agent lycobetaine and DNA. Gaojishu Tongxun. 1992; 2 30-3
- 12 Barthelems H U, Niederberger E, Roth T, Schulte K, Tang W C, Zankl H, Fiebig H H, Eisenbrand G, Marko D. Lycobetaine acts as a selective topoisomerase IIβ poison and inhibits the growth of human tumor cells. British Journal of Cancer. 2001; 85 1585-91
- 13 Zhang S Y, Lu F L, Yang J L, Wang L J, Xu B. Effect on animal tumors and toxicity of lycobetaine acetate. Acta Pharmacologica Sinica. 1981; 2 41-5
- 14 Hua D H, Saha S, Takemoto D J. Anticancer activities of 2,5,8,9-substituted 6-oxo-1,2,3,4,5,6-hexahydrophenanthridines on multi-drug-resistant phenotype cells. Anticancer Research. 1997; 17A 2435-41
- 15 Niederberger E. Mechanismus-orientierte Untersuchungen zur antineoplastischen Wirkung von Naturstoffen und Naturstoff-Derivaten. Thesis, Universtiy of Kaiserslautern Germany; 1998
- 16 Schulte K. Synthese von Phenanthridin- und Benzo[c]phenanthridin-Derivaten und Untersuchungen auf ihre biologische Wirkung. Thesis, Universtiy of Kaiserslautern Germany; 2000
- 17 Kushida N, Atsumi S, Koyano T, Umezawa K. Induction of flat morphology in K-ras-transformed fibroblasts by lycorine, an alkaloid isolated from the tropical plant Eucharis grandiflora . Drugs under Experimental and Clinical Research. 1997; 23 151-5
- 18 Fang S D, Wang L K, Hecht S M. Inhibitors of DNA topoisomerase I isolated from the roots of Zanthoxylum nitidum . Journal of Organic Chemistry. 1993; 58 5025-7
- 19 The Pharmacopoeia of the People’s Republic of China. Vol I Ed 2000 Chemical Industrial Press Beijing, PR China;
- 20 Colombo M L, Bosisio E. Pharmacological activities of Chelidonium majus L. (Papaveraceae). Pharmacological Research. 1996; 33 127-34
- 21 Stermitz F R, Gillespie J P, Amoros L G, Romero R, Stermitz T A, Larson K A, Earl S, Ogg J E. Synthesis and biological activity of some antitumor benzophenanthridinium salts. Journal of Medicinal Chemistry. 1975; 18 708-13
- 22 Fan Y J, Zhou J, Li M. Effect of nitidine chloride on the life cycle of Ehrlich ascites carcinoma cells in mice. Acta Pharmacologica Sinica. 1981; 2 46-9
- 23 Wang M H. Isolation of antitumor alkaloids from Zanthoxylum nitidum and structural study of its alkaloid C. Chinese Pharmaceutical Bulletin. 1981; 16 48
- 24 Zhu D Y. Recent advances on the active components in Chinese medicines. Abstracts of Chinese Medicines (Hong Kong). 1987; 1 251-286
- 25 Kubova N, Smekal E, Kleinwachter V, Cushman M. Binding properties of nitidine and its indenoisoquinoline analog with DNA. Studia Biophysica. 1986; 114 251-6
- 26 Gatto B, Sanders M M, Yu C, Wu H Y, Makhey D, LaVoie E J, Liu L F. Identification of topoisomerase I as the cytotoxic target of the protoberberine alkaloid coralyne. Cancer Research. 1996; 56 2795-800
- 27 Del Poeta M, Chen S F, Von Hoff D, Dykstra C C, Wani M C, Manikumar G, Heitman J, Wall M E, Perfect J R. Comparison of in vitro activities of camptothecin and nitidine derivatives against fungal and cancer cells. Antimicrobial Agents and Chemotherapy. 1999; 43 2862-78
- 28 Wang X, Henningfeld K A, Hecht S M. DNA topoisomerase I-mediated formation of structurally modified DNA duplexes. Effects of metal ions and topoisomerase I inhibitors. Biochemistry. 1998; 37 2691-700
- 29 Chen Y Z, Tang G Y, Xu B J, Wu Q J, Lu C Z, Li J Q, Huang Z X. The formation and crystal structure of dihydronitidine and discussion of anticancer mechanism of nitidine cation. Science in China Series B. 1992; 35 1101-9
- 30 Larsen A K, Grondard L, Couprie J, Desoize B, Comoe L, Jardillier J C, Riou J F. The antileukemic alkaloid fagaronine is an inhibitor of DNA topoisomerases I and II. Biochemical Pharmacology. 1993; 46 1403-12
- 31 Fleury F, Sukhanova A, Ianoul A, Devy J, Kudelina I, Duval O, Alix A J, Jardillier J C, Nabiev I. Molecular determinants of site-specific inhibition of human DNA topoisomerase I by fagaronine and ethoxidine. Relation to DNA binding. Journal of Biological Chemistry. 2000; 275 3501-9
- 32 Pezzuto J M, Antosiak S K, Messmer W M, Slaytor M B, Honig G R. Interaction of the antileukemic alkaloid, 2-hydroxy-3,8,9-trimethoxy-5-methylbenzo[c]phenanthridine (fagaronine), with nucleic acids. Chemico-Biological Interactions. 1983; 43 323-39
- 33 Herbert J M, Augereau J M, Gleye J, Maffrand J P. Chelerythrine is a potent and specific inhibitor of protein kinase C. Biochemical and Biophysical Research Communications. 1990; 172 993-9
- 34 Sampaio-Maia B, Soares-da-Silva P. Ca2+/calmodulin mediated pathways regulate the uptake of l-DOPA in mouse neuroblastoma neuro 2A cells. Life Sciences. 2000; 67 3209-20
- 35 Fernandez Y, Ramakrishnan R, Rathinavelu A. GI-101A breast tumor cell growth. Life Sciences. 2000; 67 567-75
- 36 Ramakrishnan R, Zell J A, Malave A, Rathinavelu A. Expression of vascular endothelial growth factor mRNA in GI-101A and HL-60 cell lines. Biochemical and Biophysical Research Communications. 2000; 270 709-13
- 37 Mueller H, Liu R, David F, Eppenberger U. Selective modulation of protein kinase A and protein kinase C activities in epidermal growth factor (EGF)-stimulated MCF-7 breast cancer cells. Biological Chemistry. 1997; 378 1023-9
- 38 Lamm M L, Long D D, Goodwin S M, Lee C. Transforming growth factor-β1 inhibits membrane association of protein kinase Cα in a human prostate cancer cell line, PC3. Endocrinology. 1997; 138 4657-64
- 39 Zhu G H, Wong B C, Eggo M C, Yuen S T, Lai K C, Lam S K. Pharmacological inhibition of protein kinase C activity could induce apoptosis in gastric cancer cells by differential regulation of apoptosis-related genes. Digestive Diseases and Sciences. 1999; 44 2020-6
- 40 Chmura S J, Dolan M E, Cha A, Mauceri H J, Kufe D W, Weichselbaum R R. In vitro and in vivo activity of protein kinase C inhibitor chelerythrine chloride induces tumor cell toxicity and growth delay in vivo . Clinical Cancer Research. 2000; 6 737-42
- 41 Wolff J, Knipling L. Antimicrotubule properties of benzophenanthridine alkaloids. Biochemistry. 1993; 32 13 334-9
- 42 Wang B H, Lu Z X, Polya G M. Inhibition of eukaryote protein kinases by isoquinoline and oxazine alkaloids. Planta Medica. 1997; 63 494-8
- 43 Vavreckova C, Gawlik I, Muller K. Benzophenanthridine alkaloids of Chelidonium majus. II. Potent inhibitory action against the growth of human keratinocytes. Planta Medica. 1996; 62 491-4
- 44 Ahmad N, Gupta S, Husain M M, Heiskanen K M, Mukhtar H. Differential antiproliferative and apoptotic response of sanguinarine for cancer cells versus normal cells. Clinical Cancer Research. 2000; 6 1524-8
- 45 Dai J R, Chai H, Pezzuto J M, Kinghorn A D, Tsauri S, Padmawinata K. Studies on Indonesian Plants. V. Cytotoxic constituents of the roots of the Indonesian medicinal plant Fibraurea chloroleuca . Phytotherapy Research. 1993; 7 290-4
- 46 Chen K T, Hao D M, Liu Z X, Chen Y C, You Z S. Effect of berberine alone or in combination with argon ion laser treatment on the 9L rat glioma cell line. Chinese Medicinal Journal (Engl). 1994; 107 808-12
- 47 Iizuka N, Miyamoto K, Okita K, Tangoku A, Hayashi H, Yosino S, Abe T, Morioka T, Hazama S, Oka M. Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Letters. 2000; 148 19-25
- 48 Chi C W, Chang Y F, Chao T W, Chiang S H, Peng F K, Lui W Y, Liu T Y. Flow-cytometric analysis of the effect of berberine on the expression of glucocorticoid receptors in human hepatoma HepG2 cells. Life Sciences. 1994; 54 2099-2107
- 49 Lin H L, Chang Y F, Liu T Y, Wu C W, Chi C W. Submicromolar paclitaxel induces apoptosis in human gastric cancer cells at early G1 phase. Anticancer Research. 1998; 18 3443-9
- 50 Yang I W, Chou C C, Yung B Y. Dose-dependent effects of berberine on cell cycle pause and apoptosis in Balb/c 3T3 cells. Naunyn-Schmiedebergs Archives of Pharmacology. 1996; 354 102-8
- 51 Chang K S, Gao C, Wang L C. Berberine-induced morphologic differentiation and down-regulation of c-Ki-ras2 protooncogene expression in human teratocarcinoma cells. Cancer Letters. 1990; 55 103-8
- 52 Miura N, Yamamoto M, Ueki T, Kitani T, Fukuda K, Komatsu Y. Inhibition of thymocyte apoptosis by berberine. Biochemical Pharmacology. 1997; 53 1315-22
- 53 Kuo C L, Chou C C, Yung B Y. Berberine complexes with DNA in the berberine-induced apoptosis in human leukemic HL-60 cells. Cancer Letters. 1995; 93 193-200
- 54 Ren J, Chaires J B. Sequence and structural selectivity of nucleic acid binding ligands. Biochemistry. 1999; 38 16 067-75
- 55 Lee S J, Kim J B, Lee S W, Kim J H. Enhanced cytotoxicity of berberine and some anticancer nucleotides against tumor cell-lines. Archives of Pharmacal Research. 1995; 18 138-9
- 56 Zhang R X, Dougherty D V, Rosenblum M L. Laboratory studies of berberine used alone and in combination with 1,3-bis(2-chloroethyl)-1-nitrosourea to treat malignant brain tumors. Chinese Medicinal Journal (English Edition). 1990; 103 658-65
- 57 Lin H L, Liu T Y, Wu C W, Chi C W. Berberine modulates expression of mdr1 gene product and the responses of digestive track cancer cells to Paclitaxel. British Journal of Cancer. 1999; 81 416-22
- 58 Lin H L, Liu T Y, Lui W Y, Chi C W. Up-regulation of multidrug resistance transporter expression by berberine in human and murine hepatoma cells. Cancer. 1999; 85 1937-42
- 59 Dai J R, Chai H, Pezzuto J M, Kinghorn A D, Tsauri S, Padmawinata K. Studies on Indonesian Plants. V. Cytotoxic constituents of the roots of the Indonesian medicinal plant Fibraurea chloroleuca . Phytotherapy Research. 1993; 7 290-4
- 60 Makhey D, Gatto B, Yu C, Liu A, Liu L F, LaVoie E J. Protoberberine alkaloids and related compounds as dual inhibitors of mammalian topoisomerase I and II. Medicinal Chemistry Research. 1995; 5 1-12
- 61 Kobayashi Y, Yamashita Y, Fuji N, Takaboshi K, Kawakami T, Kawamura M, Mizukami T, Nakano H. Inhibitors of DNA topoisomerase I and II isolated from the Coptis rhizomes . Planta Medica. 1995; 61 414-8
- 62 Kim S A, Kwon Y, Kim J H, Muller M T, Chung I K. Induction of topoisomerase II-mediated DNA cleavage by a protoberberine alkaloid, berberrubine. Biochemistry. 1998; 37 16 316-24
- 63 Xia G C, Zang J Y, Lu X M, Xiao P G. Resource utilization and herbal study of ”malinzi” (Iris lactea Pall. var. chinensis). Acta Pharmacentica Sinica. 1985; 20 316-9
- 64 Li D H, Hao X G, Zhang S K, Wang S X, Liu R Y, Ma K S, Yu S P, Jiang H, Guan J F. Antitumor effect and toxicity of irisquinone. Acta Pharmacologica Sinica. 1981; 2 131-4
- 65 Wang S X, Liu M F, Yuan L, Zhang W L, Li D H. Effects of irisquinone on cyclic nucleotides in plasma, cancer and other tissues of mice bearing U14 tumor. Chinese Journal of Clinical Oncology. 1986; 13 241-3
- 66 Li W M, Wang S H, Kuang P, Qu B X, Song H L, Zhu L X. Radiosensitizing effect of an active principle Iq7611 from the seed of Iris lactea var. chinenesis . Tumor. 1987; 7 97-9
- 67 Chitra M, Sukumar E, Suja V, Devi C S. Antitumor, anti-inflammatory and analgesic property of embelin, a plant product. Chemotherapy. 1994; 40 109-113
- 68 Chitra M, Sukumar E, Devi C S. [3 H]-Thymidine uptake and lipid peroxidation by tumor cells on embelin treatment: an in vitro study. Oncology. 1995; 52 66-8
- 69 Yuan K, Hu R, Ji C, Yin M. New method for preparing oridonin by column chromatography. China Journal of Chinese Materia Medica. 1997; 22 478-80, 511
- 70 Wang R L, Gao B L, Xiong M L, Mei Q D, Fan K S, Zuo Z K, Lang T L, Gao G Q, Ji Z C, Wei D C. Potentiation by Rabdosia rubescens on chemotherapy of advanced esophageal carcinoma. Chinese Journal of Oncology. 1986; 8 297-9
- 71 Wang R L. A report of 40 cases of esophageal carcinoma surviving for more than 5 years after treatment with drugs. Chinese Journal of Oncology. 1993; 15 300-2
- 72 Porterfield H. UsToo PC-SPES surveys: review of studies and update of previous survey results. Molecular Urology. 2000; 4 289-91
- 73 de la Taille A, Hayek O R, Burchardt M, Burchardt T, Katz A E. Role of herbal compounds (PC-SPES) in hormone-refractory prostate cancer: two case reports. Journal of Alternative and Complementary Medicine. 2000; 6 449-51
- 74 Lou F C, Ding L S, Ma Q Y, Du F L. Natural antineoplastic compounds and their structure-activity relationships. Journal of Nanjing College of Pharmacy. 1986; 17 152-9
- 75 Fujita T, Takeda Y, Sun H D, Minami Y, Marunaka T, Takeda S, Yamada Y, Togo T. Cytotoxic and antitumor activities of Rabdosia diterpenoids. Planta Medica. 1988; 54 414-7
- 76 Wang M Y, Lin C, Zhang T M. Cytokinetic effects of oridonin on leukemia L1210 cells. Acta Pharmacologica Sinica. 1985; 6 195-8
- 77 Li X T, Lin C, Li P Y. Comparison of in vitro assays for the cytotoxic effect of anticancer drugs. Chinese Journal of Oncology. 1986; 8 184-6
- 78 Yu J J, Reed E. Preliminary study of the effect of selected Chinese natural drugs on human ovarian cancer cells. Oncology Reporter. 1995; 2 571-5
- 79 Wang M Y, Lin C, Zhang T M. Autoradiographic study on the effects of oridonin on DNA, RNA and protein synthesis of leukemia L 1210 cells. Acta Pharmacologica Sinica. 1987; 8 164-5
- 80 Li Y, Zhang T M. Effect of oridonin on cell-free DNA synthesis in vitro . Acta Pharmacologica Sinica. 1988; 9 465-7
- 81 Gao Z G, Ye Q X, Zhang T M. Synergistic effect of oridonin and cisplatin on cytotoxicity and DNA cross-link against mouse sarcoma S180 cells in culture. Acta Pharmacologica Sinica. 1993; 14 561-4
- 82 Zhang T M, Shou M G, Wang M Y. Antitumor effects of different combinations of oridonin, bleomycin A5 and nitrocaphane. Acta Pharmacologica Sinica. 1986; 7 457-60
- 83 Jiang B, Lu Z Q, Hou A J, Zhao Q S, Sun H D. ent-Kaurane diterpenoids from Isodon lungshengensis . Journal of Natural Products. 1999; 62 941-5
- 84 Sun H D, Lin Z W, Niu F D, Lin L Z, Chai H B, Pezzuto J M, Cordell G A. Cytotoxic ent-kaurene diterpenoids from three Isodon species. Phytochemistry. 1995; 38 437-42
- 85 Fujita E, Nagao y, Node M, Kaneko K, Nakazawa S, Kuroda H. Antitumor activity of the Isodon diterpenoids; structural requirements for the activity. Experientia. 1976; 32 203-6
- 86 Zhai J K, Han W C, Ju X H. Crystal structure of nervosin and the electronic structure of its anticancer-active zone. Acta Chimica Sinica. 1993; 51 854-9
- 87 Fujita E, Nagao Y, Kaneko K, Nakazawa S, Kuroda H. The antitumor and antibacterial activity of the Isodon diterpenoids. Chemical and Pharmaceutical Bulletin (Tokyo). 1976; 24 2118-27
- 88 Hall I H, Lee K H, Okano M, Sims D, Ibuka T, Liou Y F, Imakura Y. Antitumor agents. XLII. Comparison of antileukemic activity of helenalin, brusatol and bruceantin and their esters on different strains of P388 lymphocytic leukemic cells. Journal of Pharmaceutical Sciences. 1981; 70 1147-50
- 89 Liao L L, Kupchan S M, Horwith S B. Mode of action of the antitumor compound bruceantin, an inhibitor of protein synthesis. Molecular Pharmacology. 1976; 12 167-76
- 90 Garnick M B, Blum R H, Canellos G P, Mayer R J, Parker L, Skarin A T, Li F P, Henderson I C, Frei E. Phase I trial of bruceantin. Cancer Treatment Reports. 1979; 63 1929-32
- 91 Amato D A, Borden E C, Shiraki M, Enterline H AT, Rosenbaum C, Davis H L, Paul A R, Stevens C M, Lerner H J. Evaluation of bleomycin, chlorozotocin, MGBG, and bruceantin in patients with advanced soft tissue sarcoma, bone sarcoma, or mesothelioma. Investigational New Drugs. 1985; 3 397-401
- 92 Wiseman C L, Yap H Y, Bedikian A Y, Bodey G P, Blumenschein G R. Phase II trial of bruceantin in metastatic breast carcinoma. American Journal of Clinical Oncology. 1982; 5 389-91
- 93 Cheng J H, Long H, Zu J L. Therapeutic analysis of treatment of recurrent metastatic gastrointestinal carcinomas in 35 patients with Brucea javanica emulsion ”Yandanziyou”. Chinese Traditional Patent Medicine. 1991; 13 21-2
- 94 Wang Z Q. Combined therapy of brain metastasis in lung cancer. Chinese Journal of Integrated Traditional and Western Medicine. 1992; 12 609-10
- 95 Lu J B, Shu S Y, Cai J Q. Experimental study on effect of Brucea javanica oil emulsion on rabbit intracranial pressure. Chinese Journal of Integrated Traditional and Western Medicine. 1994; 14 610-11
- 96 Wang J Z, Chen D Y, Su Y Y. Analytical study on processing of Scutellaria baicalensis Georgi by HPLC. China Journal of Chinese Materia Medica. 1994; 19 340-1
- 97 Tomimori T, Miyaichi Y, Kizu H. On the flavonoid constituents from the roots of Scutellaria baicalensis Georgi. I. Yakugaku Zasshi. 1982; 102 388-91
- 98 Motoo Y, Sawabu N. Antitumor effects of saikosaponins, baicalin and baicalein on human hepatoma cell lines. Cancer Letters. 1994; 86 91-5
- 99 Ikemoto S, Sugimura K, Yoshida N, Yasumoto R, Wada S, Yamamoto K, Kishimoto T. Antitumor effects of scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines. Urology. 2000; 55 951-5
-
100 Niederberger E, Meiers S, Genzlinger A, Zankl H, Tang W C, Eisenbrand G, Marko D. Flavones and inhibition of tumor cell growth: new aspects on the mechanism of action. In: Eisenbrand G, Dayan AD, Elias PS, Grunow W, Schlatter J, editors
Carcinogenic and anticarcinogenic factors in food . Wiley-VCH Weinheim; 1998: 512-3 - 101 Matsuzaki Y, Kurokawa N, Terai S, Matsumura Y, Kobayashi N, Okita K. Cell death induced by baicalein in human hepatocellular carcinoma cell lines. Japanese Journal of Cancer Research. 1996; 87 170-7
- 102 Austin C A, Patel S, Ono K, Nakane H, Fisher L M. Site-specific DNA cleavage by mammalian DNA topoisomerase II induced by novel flavone and catechin derivatives. Biochemical Journal. 1992; 282 883-9
- 103 Chan F L, Choi H L, Chen Z Y, Chan P S, Huang Y. Induction of apoptosis in prostate cancer cell lines by a flavonoid, baicalin. Cancer Letters. 2000; 160 219-28
- 104 Huang H C, Hsieh L M, Chen H W, Lin Y S, Chen J S. Effects of baicalein and esculetin on transduction signals and growth factors expression in T-lymphoid leukemia cells. European Journal of Pharmacology. 1994; 268 73-8
- 105 So F V, Guthrie N, Chambers A F, Moussa M, Carroll K K. Inhibition of human breast cancer cell proliferation and delay of mammary tumorigenesis by flavonoids and Citrus juices. Nutrition and Cancer. 1996; 26 167-81
- 106 So F V, Guthrie N, Chambers A F, Carroll K K. Inhibition of proliferation of estrogen receptor-positive MCF-7 human breast cancer cells by flavonoids in the presence and absence of excess estrogen. Cancer Letters. 1997; 112 127-33
- 107 Chang W S, Lee Y J, Lu F J, Chiang H C. Inhibitory effects of flavonoids on xanthine oxidase. Anticancer Research. 1993; 13 2165-70
- 108 Umezawa K. Inhibition of experimental metastasis by enzyme inhibitors from microorganisms and plants. Advances in Enzyme Regulation. 1996; 36 267-81
- 109 Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. Antiproliferative activity of flavonoids on several cancer cell lines. Bioscience, Biotechnology and Biochemistry. 1999; 63 896-9
- 110 Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. Effect of Citrus flavonoids on HL-60 cell differentiation. Anticancer Research. 1999; 19(A) 1261-9
- 111 Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. HL-60 differentiating activity and flavonoid content of the readily extractable fraction prepared from Citrus juices. Journal of Agricultural and Food Chemistry. 1999; 47 128-35
- 112 Bracke M, Vyncke B, Opdenakker G, Foidart J M, De Pestel G, Mareel M. Effect of catechins and citrus flavonoids on invasion in vitro . Clinical and Experimental Metastasis. 1991; 9 13-25
- 113 Takanaga H, Ohnishi A, Yamada S, Matsuo H, Morimoto S, Shoyama Y, Ohtani H, Sawada Y. Polymethoxylated flavones in orange juice are inhibitors of P-glycoprotein but not cytochrome P450 3A4. Journal of Pharmacology and Experimental Therapeutics. 2000; 293 230-6
- 114 Ikegawa T, Ushigome F, Koyabu N, Morimoto S, Shoyama Y, Naito M, Tsuruo T, Ohtani H, Sawada Y. Inhibition of P-glycoprotein by orange juice components, polymethoxyflavones in adriamycin-resistant human myelogenous leukemia (K562/ADM) cells. Cancer Letters. 2000; 160 21-8
- 115 Institute of H aematology, Chinese Academy of Medical S ciences. Clinical studies of Dang Gui Lu Hui Wan in the treatment of CML. Chinese Journal of Internal Medicine. 1979; 15 86-8
- 116 Wu L M, Yang Y P, Zhu Z H. Studies on the active principles of Indigofera tinctoriain the treatment of CML. Comm Chinese Herb Med. 1979; 9 6-8
- 117 Ji X J, Zhang F R, Lei J L, Xu Y T. Studies on the antineoplastic effect and toxicity of synthetic indirubin. Acta Pharmacentica Sinica. 1981; 16 146-8
- 118 Indirubin cooperative g roup. Clinical study of indirubin in the treatment of 314 patients with chronic granulocytic leukemia. Chinese Journal of Hematology. 1980; 1 132
- 119 Gan W J, Yang T Y, Wen S D, Liu Y Y, Tan Z, Deng C A, Wu J X, Liu M P. Studies on the mechanism of indirubin action in treatment of chronic myelocytic leukemia (CML). II. 5′-Nucleotidase in the peripheral white blood cells of CML. Chinese Journal of Hematology. 1985; 6 611-3
- 120 Qian L S, Xue Y P, Zhang X M, Yang T Y, Yan W W, Wang Z C. Observation on the long period effect of indirubin in the treatment of 57 cases of chronic myelogenous leukemia. Chinese Journal of Hematology. 1991; 12 125-7
- 121 Zhang L, Wu G Y, Qiu C C. Effect of indirubin on DNA synthesis in vitro . Acta Academiae Medicinae Sinicae. 1985; 7 112-6
- 122 Gan W J, Yang T Y, Wang Z C, Qian L S, Ma J, Ge Y Q, Cheng B J, Li Z M, Bo H Q. Studies on the antitumor mechanism of indirubin by treatment of chronic myelocytic leukemia (CML). Chinese Biochemical Journal. 1987; 3 225-30
- 123 Du D J, Ceng Q T. Effect of indirubin on the incorporation of isotope labeled precursors into nucleic acid and protein of tumor tissues. Chinese Traditional and Herbal Drugs. 1981; 12 406-9
- 124 Du D G, Ceng Q T, Wen Z S, Wan X P. Study on the incorporation of 3H-TdR into DNA of liver and spleen of L7212, a lymphatic leukemia in mice. Chinese Journal of Hematology. 1982; 3 277-80
- 125 Wu G Y, Liu J Z, Fang F D, Zuo J. Studies on the mechanism of indirubin action in the treatment of chronic granulocytic leukemia. V. Binding between indirubin and DNA and identification of the type of binding. Scientia Sinica. 1982; 25B 1071-9
- 126 Lee K, Shih C Y, Yang T Y, Chen L S, Chao W M, Sun C S, Wang T C, Pien S K, Sing K H. Ultratherapeutic effect of indirubin for human chronic granulocytic leukemia. National Medical Journal of China. 1979; 59 129-32
- 127 You Y C, Mi J X, Wan J H, Yang T Y, Wang Z C, Qian L S. Indirubin in the treatment of chronic myelocytic leukemia (CML) estimation of labelling index of bone marrow cells by 3H-TdR. Chinese Journal of Oncology. 1987; 9 418-20
- 128 Chen K Y, Wang Z F, Li C Y, Li W X. Study on indirubin and indigo by ESR spectrometry. Chemical Journal of Chinese Universities. 1989; 10 869-71
- 129 Hoessel R, Leclerc S, Endicott J A, Nobel M E, Lawrie A, Tunnah P, Leost M, Damiens E, Marie D, Marko D, Niederberger E, Tang W, Eisenbrand G, Meijer L. Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases. Nature Cell Biology. 1999; 1 60-7
- 130 Marko D, Schatzle S, Friedel A, Genzlinger A, Zankl H, Meijer L, Eisenbrand G. Inhibition of cyclin-dependent kinase 1 (CDK1) by indirubin derivatives in human tumour cells. British Journal of Cancer. 2001; 84 283-9
- 131 Leclerc S, Garnier M, Hoessel R, Marko D, Bibb J A, Snyder G L, Greengard P, Biernat J, Wu Y Z, Mandelkow E M, Eisenbrand G, Meijer L. Indirubins inhibit glycogen synthase kinase-3β and CDK5/p25, two protein kinases involved in abnormal tau phosphorylation in Alzheimer’s disease. A property common to most cyclin-dependent kinase inhibitors?. Journal of Biological Chemistry. 2001; 276 251-60
- 132 Sielecki T M, Boylan J F, Benfield P A, Trainor G L. Cyclin-dependent kinase inhibitors: useful targets in cell cycle regulation. Journal of Medicinal Chemistry. 2000; 43 1-18
- 133 Buolamwini J K. Cell cycle molecular targets in novel anticancer drug discovery. Current Pharmaceutical Design. 2000; 6 379-92
- 134 Wang J H, You Y C, Mi J X, Ying H G. Effect of indirubin on hematopoietic cell production. Acta Pharmacologica Sinica. 1981; 2 241-4
- 135 Wen Z J, Liu Y, Yi Z P, Yang Y S. Effects of indirubin on the histology and histochemistry of canine and rat livers. Bulletin of Chinese Materia Medica. 1988; 13 306-7
- 136 Sichnan Institute of Traditional Chinese M edicine. Subacute toxicity of indirubin in dogs. Chinese Traditional and Herbal Drugs. 1981; 12 27-9
- 137 Cai Y Y, Xu C L, Li S H, Liu Y. Studies on sister chromatid exchanges induced by harringtonine, indirubin and pyquiton before or after activation with microsome enzyme. Acta Academiae Medicinae Sinicae. 1983; 5 161-4
- 138 Feng B Z, Zhang Y H, Qian L S, Chu Y L. Effect of indirubin on SCE frequencies of BM cells in chronic myeloid leukemia. Acta Academiae Medicinae Sinicae. 1984; 6 308-10
- 139 Feng B Z. Sister chromatid exchange frequency of bone marrow cells and its response to indirubin in chronic myeloid leukemia. Chinese Journal of Oncology. 1984; 6 357-60
- 140 Wu K M, Zhang M Y, Fang Z, Huang L. Synthesis of N 1-substituted derivatives of indirubin, an antileukemic compound. Acta Pharmacentica Sinica. 1984; 19 513-8
- 141 Ji XJ Zhang F R. Antineoplastic effect of indirubin derivatives and their structure-activity relationship. Acta Pharmacentica Sinica. 1985; 20 137-9
- 142 Zeng Q T, Du D J, Xie D C, Wang X P, Rau C Q. Antitumor activities of indirubin derivatives. Chinese Traditional and Herbal Drugs. 1982; 13 24-30
- 143 Gu Y C, Li G L, Yang Y P, Fu J P, Li C Z. Synthesis of some halogenated indirubin derivatives. Acta Pharmacentica Sinica. 1989; 24 629-32
- 144 Zeng Q T, Du D J, Si X F, Ran C Q, Wu X P. Antitumor activity of 5′-iodoindirubin. West China Pharm Sci. 1991; 6 63-8
- 145 Wu G Y, Liu J Z, Zhang L. Effect of bisindole compounds with three different kinds of linkage on the synthesis of nucleic acid and protein. Progress in Biochemistry and Biophysics. 1985; 61 48-51
- 146 Wu K M, Zhang M Y, Fang Z, Huang L. Potential antileukemic agents, synthesis of derivatives of indirubin, indigo, and isoindigotin. Acta Pharmacentica Sinica. 1985; 20 821-6
- 147 Ji X J, Liu X M, Li K, Chen R H, Wang L G. Pharmacological studies of meisoindigo: absorption and mechanism of action. Biomedical and Environmental Sciences. 1991; 4 332-7
- 148 Liu X M, Wang L G, Li H Y, Ji X J. Induction of differentiation and down-regulation of c-myb gene expression in ML-1 human myeloblastic leukemia cells by the clinically effective anti-leukemia agent meisoindigo. Biochemical Pharmacology. 1996; 51 1545-51
Dr. Barbara Bertram
Toxicology and Cancer Risk Factors
Deutsches Krebsforschungszentrum
Im Neuenheimer Feld 280
69120 Heidelberg
Germany
eMail: b.bertram@dkfz.de
Fax: +49 6221-423031
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 1 Lycobetaine and lycorine.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 2 Nitidine, fagaronine, and chelerythrine.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 3 Berberine, epiberberine, berberrubine, and groenlandicine.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 4 Irisquinone A and embelin.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 5 Oridonin and ponicidin.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 6 Bruceantin and brusatol.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 7 Baicalein, wogonin, and skullcapflavone II.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 8 Tangeretin, nobiletin, sinensetin, and auranetin.
![Zoom Image](/products/assets/desktop/css/img/icon-figure-zoom.png)
Fig. 9 Indirubin, indigo, and isoindigo.