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DOI: 10.1055/s-2003-38881
Glucosinolates in Moringa stenopetala
Publication History
Received: August 28, 2002
Accepted: December 15, 2002
Publication Date:
23 April 2003 (online)
Abstract
Bio-guided fractionation of seed extracts from Moringa stenopetala resulted in a myrosinase hydrolysis product, 5,5-dimethyloxazolidine-2-thione. It is formed from the glucosinolate glucoconringiin, which was identified together with O-(rhamnopyranosyloxy)benzyl glucosinolate from M. stenopetala for the first time. The glucosinolates in seeds, leaves and roots of M. stenopetala were quantified as des-sulphoglucosinolate by HPLC. The seeds without testa contained the highest concentration of glucoconringiin and of O-(rhamnopyranosyloxy)benzyl glucosinolate within the plant, 3 % and 19 % of dry mass, respectively.
Abbreviations
GLS:glucosinolate
GC:glucoconringiin
RB-GLS:O-(rhamnopyranosyloxy)benzyl glucosinolate
References
- 1 Jahn S AA. Using Moringa seeds as coagulants in developing countries. J Am Water Works Assoc. 1988; 80 43-50
- 2 Mekonnen Y, Gessesse A. Documentation of the uses of Moringa stenopetala and its possible antileishmanial and antifertility effects. Ethiop J Sci. 1998; 21 287-95
- 3 Mekonnen Y, Yardley V, Rock P, Croft S. In vitro antitrypanosomal activity of Moringa stenopetala leaves and roots. Phytother Res. 1999; 13 538-9
- 4 Mekonnen Y. Effects of ethanol extract of Moringa stenopetala leaves on guinea-pig and mouse smooth muscle. Phytothe Res. 1999; 13 442-4
- 5 Eilert U, Wolters B. Nahrstedt A. The antibiotic principle of seeds of Moringa oleifera and Moringa stenopetala . Planta Med. 1981; 42 55-61
- 6 Fahey J W, Zalcmann A T, Talalay P. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry. 2001; 56 5-51
- 7 Rosa E AS, Heaney R K, Fenwick G R, Porta C AM. Glucosinolate in crop plants. Horticultural Reviews. 1997; 19 99-215
- 8 Kjaer A, Malver O, ElMenshari B, Reisch J. Isothiocyantes in myrosinase-treated seed extracts of Moringa peregrina . Phytochemistry. 1979; 18 1485-7
- 9 Daxenbichler M E, Spencer G F, Carlson D G, Rose G B, Brinker A M, Powell R G. Glucosinolate composition of seeds from 297 species of wild plants. Phytochemistry. 1991; 30 2623-38
- 10 Kiddle G, Bennett R N, Botting N P, Davidson N E, Robertson-Avril A B, Wallsgrove R M. High-performance liquid chromatographic separation of natural and synthetic desulphoglucosinolates and their chemical validation by UV, NMR and chemical ionisation-MS methods. Phytochem Anal. 2001; 12 226-42
- 11 Matthaeus B, Luftmann H. Glucosinolates in members of the family Brassicaceae: Separation and identification by LC/ESI-MS-MS. J Agric Food Chem. 2000; 48 2234-9
- 12 Badgett B L. Ph. D. Thesis. University of Houston Texas,; cited after Kjaer et al. 1979 (Ref. 8) 1964
- 13 Muluvi G M, Sprent J I, Soranzo N, Provan J, Odee D, Folkard G, McNicol J W, Powell W. Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam. Molecular Ecology. 1999; 8 470
- 14 Faizi S, Siddiqui B S, Saleem R, Siddiqui S, Aftag K, Gilani A H. Novel hypotensive agents, niazimin A, niazimin B, niazicin A and niazicin B from Moringa oleifera: Isolation of first naturally occuring carbamates. J Chem Soc 1994: 3035-40
- 15 Faizi S, Siddiqui B S, Saleem R, Aftab K, Shaheen F, Gilani A H. Hypotensive constituents from the pods of Moringa oleifera . Planta Med. 1998; 64 225-8
- 16 Murakami A, Kitazono Y, Jiwajinda S, Koshimizu K, Ohigathi H. Niaziminin, a thiocarbamate from the leaves of Moringa oleifera, holds a strict structural requirement for inhibition of tumor-promoter-induced Epstein-Barr virus activation. Planta Med. 1998; 64 319-23
- 17 Guevara A P, Vargas C, Sakurai H, Fujiwara Y, Hashimoto K, Maoka T, Kozuka M, Ito Y, Tokuda H, Nishino H. An antitumor promoter from Moringa oleifera Lam. Mutation Research. 1999; 440 181-8
- 18 van Poppel G, Verhoeven D T, Verhagen H, Goldbohm R A, Brassica vegetables and cancer p revention. Epidemiology and mechanisms. Adv Exp Med Biol. 1999; 472 159-68
- 19 Gassenschmidt U, Jany K D, Tauscher B, Niebergall H. Isolation and characterization of a flocculating protein from Moringa oleifera Lam. Biochim Biophys Acta. 1995; 1243 477-81
-
20 Buchner R. Approach to determination of HPLC response factors for glucosinolates. In: Wathelet, J-P, editor
World Crops: Production, Utilisation, Description. Vol. 13 Glucosinolates in rapeseeds : Analytical aspects . Boston; Martinuns Nijhoff Publishers 1987: p 50-8
Dr. Birgit Dräger
Institute of Pharmaceutical Biology
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