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Horm Metab Res 2003; 35(2): 81-85
DOI: 10.1055/s-2003-39055
Original Basic
© Georg Thieme Verlag Stuttgart · New York

Leptin Fully Suppresses Acetylcholine-Induced Insulin Secretion and is Reversed by Tolbutamide in Isolated Perfused Chicken Pancreas

Y.  Benomar1 , N.  Rideau1 , S.  Crochet1 , M.  Derouet1 , M.  Taouis1
  • 1Station de Recherches Avicoles, INRA, F37380 Nouzilly
Further Information

Publication History

Received 24 April 2002

Accepted after revision 28 August 2002

Publication Date:
07 May 2003 (online)

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Abstract

So far, there has been no evidence for any direct pancreatic effect of leptin in the chicken. The present study was aimed at detecting chicken leptin receptor (cOb-R) expression in isolated chicken islets of Langerhans and to examine the direct effect of leptin on insulin secretion after stimulation by acetylcholine (1 µM) + glucose (14 mM) from isolated perfused chicken pancreas. We will show that i) full length cOb-R mRNA was expressed in isolated pancreatic islets of chickens, ii) recombinant chicken leptin (10 nM) or diazoxide (100 µM) rapidly (within 2 min) and significantly suppressed insulin secretion induced by acetylcholine stimulation without any change in volume outflow rate, iii) tolbutamide (100 µM) introduced 10 min after leptin and perfused for 10 min fully reversed the suppressive effect of leptin on pre-established acetylcholine-induced insulin release. In conclusion, we found that leptin has a profound inhibitory influence upon insulin secretion in perfused chicken pancreas. The results suggest that leptin inhibits insulin secretion by acting before or at the level of KATP channels in chicken pancreatic β-cells. Further studies are warranted to clarify the specific inhibitory mechanism.

Key words

Chicken - Isolated Islets - Leptin Receptor - Insulin

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N. Rideau

Station de Recherches Avicoles, INRA

F37380 Nouzilly · France

Phone: + 33 (42) 47 79 40

Fax: + 33 (42) 47 77 78

Email: rideau@tours.inra.fr